MITF Antibody (PCRP-MITF-1D9)

Novus Biologicals | Catalog # NBP3-13968

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # PCRP-MITF-1D9
View all MITF Primary Antibodies »

Product Specifications

Immunogen

Recombinant full-length human MITF protein (Uniprot: O75030)

Reactivity Notes

Predicted to react in Chick, Mouse, Rat, Xenopus and Zebrafish.

Localization

Nucleus

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14121)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. Non-hazardous.

Theoretical molecular weight: 52-56kDa (doublet)

Scientific Data Images for MITF Antibody (PCRP-MITF-1D9)

Immunocytochemistry/ Immunofluorescence: MITF Antibody (PCRP-MITF-1D9) [NBP3-13968]

Immunocytochemistry/ Immunofluorescence: MITF Antibody (PCRP-MITF-1D9) [NBP3-13968]

Immunocytochemistry/Immunofluorescence: MITF Antibody (PCRP-MITF-1D9) [NBP3-13968] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using MITF Antibody (PCRP-MITF-1D9) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).
Flow Cytometry: MITF Antibody (PCRP-MITF-1D9) [NBP3-13968]

Flow Cytometry: MITF Antibody (PCRP-MITF-1D9) [NBP3-13968]

Flow Cytometry: MITF Antibody (PCRP-MITF-1D9) [NBP3-13968] - Flow cytometric analysis of PFA-fixed HeLa cells. MITF antibody (PCRP-MITF-1D9) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).
Protein Array: MITF Antibody (PCRP-MITF-1D9) [NBP3-13968]

Protein Array: MITF Antibody (PCRP-MITF-1D9) [NBP3-13968]

Protein Array: MITF Antibody (PCRP-MITF-1D9) [NBP3-13968] - Analysis of Protein Array containing more than 19,000 full-length human proteins using MITF Antibody (PCRP-MITF-1D9).

Applications for MITF Antibody (PCRP-MITF-1D9)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunoprecipitation

1-2 ug/100-500 ug protein

Western Blot

1-2 ug/ml
Application Notes
Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: MITF

MITF (microphthalmia-associated transcription factor) is a melanocytic nuclear protein that contains basic helix-loop-helix (HLH) and leucine zipper (LZ) domains. These protein motifs are frequently observed in other transcription factors and are particularly common to members of the Myc family. MITF can directly associate with DNA as a homodimer. It is required for the development and differentiation of melanocytes. Its expression is upregulated by cAMP and cAMP dependent pathways. MITF activates several different gene promoters by binding to their E-boxes. Tyrosinase, TRP-1 and TRP-2 are pigment synthesis genes activated by MITF. When MITF is phosphorylated on Serine 73 (via the MAPK pathway), it associates with coactivators of the p300/CBP family and enhances transcription. MITF has several isoforms including MITF-M which is specifically expressed in melanocytes. In Mitfdeficient mice there is a complete absence of melanocytes.

Long Name

Microphthalmia-associated Transcription Factor

Alternate Names

bHLHe32, MI, WS2A

Gene Symbol

MITF

UniProt

O75030 (Human)

Additional MITF Products

Product Documents for MITF Antibody (PCRP-MITF-1D9)

Certificate of Analysis

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Product Specific Notices for MITF Antibody (PCRP-MITF-1D9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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