MITF Antibody (PCRP-MITF-1D9) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-14121

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # PCRP-MITF-1D9

Format

Azide and BSA Free
View all MITF Primary Antibodies »

Product Specifications

Immunogen

Recombinant full-length human MITF protein (Uniprot: O75030)

Reactivity Notes

Predicted to react in Chick, Mouse, Rat, Xenopus and Zebrafish.

Localization

Nucleus

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13968).

Antibody without azide - store at -20 to -80C. Antibody with azide - store at 2 to 8C. Non-hazardous.

Theoretical molecular weight: 52-56kDa (doublet)

Scientific Data Images for MITF Antibody (PCRP-MITF-1D9) - Azide and BSA Free

Protein Array: MITF Antibody (PCRP-MITF-1D9) - Azide and BSA Free [NBP3-14121]

Protein Array: MITF Antibody (PCRP-MITF-1D9) - Azide and BSA Free [NBP3-14121]

Protein Array: MITF Antibody (PCRP-MITF-1D9) - Azide and BSA Free [NBP3-14121] - Analysis of Protein Array containing more than 19,000 full-length human proteins using MITF Antibody (PCRP-MITF-1D9).
MITF Antibody (PCRP-MITF-1D9) - Azide and BSA Free Flow Cytometry: MITF Antibody (PCRP-MITF-1D9) - Azide and BSA Free [NBP3-14121] -

Flow Cytometry: MITF Antibody (PCRP-MITF-1D9) - Azide and BSA Free [NBP3-14121] -

Flow cytometric analysis of PFA-fixed HeLa cells. MITF Antibody (PCRP-MITF-1D9) - Azide and BSA Free followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).
MITF Antibody (PCRP-MITF-1D9) - Azide and BSA Free Flow Cytometry: MITF Antibody (PCRP-MITF-1D9) - Azide and BSA Free [NBP3-14121] -

Flow Cytometry: MITF Antibody (PCRP-MITF-1D9) - Azide and BSA Free [NBP3-14121] -

Flow cytometric analysis of PFA-fixed HeLa cells. MITF Antibody (PCRP-MITF-1D9) - Azide and BSA Free followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Applications for MITF Antibody (PCRP-MITF-1D9) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunoprecipitation

1-2 ug per 100-500 ug protein

Western Blot

1-2 ug/ml
Application Notes
Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: MITF

MITF (microphthalmia-associated transcription factor) is a melanocytic nuclear protein that contains basic helix-loop-helix (HLH) and leucine zipper (LZ) domains. These protein motifs are frequently observed in other transcription factors and are particularly common to members of the Myc family. MITF can directly associate with DNA as a homodimer. It is required for the development and differentiation of melanocytes. Its expression is upregulated by cAMP and cAMP dependent pathways. MITF activates several different gene promoters by binding to their E-boxes. Tyrosinase, TRP-1 and TRP-2 are pigment synthesis genes activated by MITF. When MITF is phosphorylated on Serine 73 (via the MAPK pathway), it associates with coactivators of the p300/CBP family and enhances transcription. MITF has several isoforms including MITF-M which is specifically expressed in melanocytes. In Mitfdeficient mice there is a complete absence of melanocytes.

Long Name

Microphthalmia-associated Transcription Factor

Alternate Names

bHLHe32, MI, WS2A

Gene Symbol

MITF

Additional MITF Products

Product Documents for MITF Antibody (PCRP-MITF-1D9) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for MITF Antibody (PCRP-MITF-1D9) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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