Mitochondria Antibody (113-1) [Alexa Fluor® 488]

Novus Biologicals | Catalog # NBP2-34517AF488

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse (Negative), Rat (Negative)

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 113-1
View all Mitochondria Primary Antibodies »

Product Specifications

Immunogen

Semi-Purified mitochondrial preparation

Reactivity Notes

Does not react with Mouse or Rat.

Localization

Mitochondria in cytoplasm

Marker

Marker for Human Cells, Granular RCC s & Salivary Tumor

Specificity

This monoclonal antibody recognizes a 60kDa antigen associated with the mitochondria in human cells. It can be used to stain mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells. This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells. Immunostaining pattern with anti-mitochondrial monoclonal antibody has been reported as a useful discriminatory adjunct in the complex differential diagnosis of granular renal cell tumors. Reportedly, this monoclonal antibody facilitates the classification of salivary tumors.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Mitochondria Antibody (113-1) [Alexa Fluor® 488]

Mitochondria Antibody (113-1) [Alexa Fluor® 488]

Immunohistochemistry-Frozen: Mouse Monoclonal Mitochondria Antibody (113-1) [Alexa Fluor® 488] [NBP2-34517AF488]

Immunohistochemistry-Frozen: Mouse Monoclonal Mitochondria Antibody (113-1) [Alexa Fluor® 488] [NBP2-34517AF488] - H2030 cells (human lung tumor cell line) have been injected intracranially in immunodeficient mice pups to form tumors. NBP2-34517AF488 (green) detects human cells in murine brain. Nuclei have been stained with Hoechst (blue). Image from a verified customer review.

Applications for Mitochondria Antibody (113-1) [Alexa Fluor® 488]

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NBP2-34517AF488 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Mitochondria

NB600-556 recognizes a 60kDa non-glycosylated protein component of mitochondria found in human cells. It can be used to stain the mitochondria in cell or tissue preparations and can be used as a marker of the mitochondria in subcellular fractions. This monoclonal antibody is part of a new panel of reagents which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb-2 produces a spaghetti-like staining pattern in the cytoplasm of normal and malignant human cells and may be used to stain the mitochondria in paraformaldehyde fixed frozen and formalin-fixed, paraffin-embedded tissues.

Additional Mitochondria Products

Product Documents for Mitochondria Antibody (113-1) [Alexa Fluor® 488]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Mitochondria Antibody (113-1) [Alexa Fluor® 488]

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Mitochondria Antibody (113-1) [Alexa Fluor® 488] (1)

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  • Mitochondria Antibody (113-1) [Alexa Fluor® 488]
    Name: Pauline Jeannot
    Application: Immunohistochemistry-Frozen
    Sample Tested: Brain metastasis (lung tumor)
    Species: Human
    Verified Customer | Posted 08/21/2024
    H2030 cells (human lung tumor cell line) have been injected intracranially in immunodeficient mice pups to form tumors. NBP2-34517AF488 (green) detects human cells in murine brain. Nuclei have been stained with Hoechst (blue)
    Mitochondria Antibody (113-1) [Alexa Fluor® 488] NBP2-34517AF488

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Protocols

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