Mitochondria Antibody (113-1)

Novus Biologicals | Catalog # NBP2-32980

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Key Product Details

Species Reactivity

Validated:

Human, Mouse (Negative), Rat (Negative)

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 113-1
View all Mitochondria Primary Antibodies »

Product Specifications

Immunogen

Semi-Purified mitochondrial preparation

Reactivity Notes

Does not react with Mouse or Rat.

Localization

Mitochondria in cytoplasm

Marker

Marker for Human Cells, Granular RCC s & Salivary Tumor

Specificity

This monoclonal antibody recognizes a 60kDa antigen associated with the mitochondria in human cells. It can be used to stain mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells. This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells. Immunostaining pattern with anti-mitochondrial monoclonal antibody has been reported as a useful discriminatory adjunct in the complex differential diagnosis of granular renal cell tumors. Reportedly, this monoclonal antibody facilitates the classification of salivary tumors.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0 mg/ml. (NBP2-34517)

Antibody with azide - store at 2 to 8C.

Scientific Data Images for Mitochondria Antibody (113-1)

Western Blot: Mitochondria Antibody (113-1) [NBP2-32980]

Western Blot: Mitochondria Antibody (113-1) [NBP2-32980]

Western Blot: Mitochondria Antibody (113-1) [NBP2-32980] - Western Blot analysis of HeLa cell lysate using Mitochondria Antibody (113-1).
Immunocytochemistry/ Immunofluorescence: Mitochondria Antibody (113-1) [NBP2-32980]

Immunocytochemistry/ Immunofluorescence: Mitochondria Antibody (113-1) [NBP2-32980]

Immunocytochemistry/Immunofluorescence: Mitochondria Antibody (113-1) [NBP2-32980] - Confocal immunofluorescence image of HeLa cells using labeled is Green (CF488) and Red Dot is used to label the nuclei Red.
Immunohistochemistry-Paraffin: Mitochondria Antibody (113-1) [NBP2-32980]

Immunohistochemistry-Paraffin: Mitochondria Antibody (113-1) [NBP2-32980]

Immunohistochemistry-Paraffin: Mitochondria Antibody (113-1) [NBP2-32980] - Immunohistochemistry-Paraffin: Mitochondria Antibody (113-1) - Azide and BSA Free [NBP2-34517] - Formalin-fixed paraffin-embedded human Renal cell carcinoma stained with Mitchondria Monoclonal antibody (113-1). Image using the Azide and BSA Free form of this antibody.
Immunohistochemistry-Paraffin: Mitochondria Antibody (113-1) [NBP2-32980]

Immunohistochemistry-Paraffin: Mitochondria Antibody (113-1) [NBP2-32980]

Immunohistochemistry-Paraffin: Mitochondria Antibody (113-1) [NBP2-32980] - Analysis using the Biotin conjugate of NBP2-32980. Staining of human prostate cancer tissue (left) and mouse stomach tissue (right). Image from verified customer review.
Immunohistochemistry-Paraffin: Mitochondria Antibody (113-1) [NBP2-32980]

Immunohistochemistry-Paraffin: Mitochondria Antibody (113-1) [NBP2-32980]

Immunohistochemistry-Paraffin: Mitochondria Antibody (113-1) [NBP2-32980] - Formalin-fixed, paraffin-embedded human testicular carcinoma stained with mitochondria monoclonal antibody (113-1). Image using the Azide and BSA Free form of this antibody.
Immunohistochemistry-Paraffin: Mitochondria Antibody (113-1) [NBP2-32980]

Immunohistochemistry-Paraffin: Mitochondria Antibody (113-1) [NBP2-32980]

Immunohistochemistry-Paraffin: Mitochondria Antibody (113-1) [NBP2-32980] - Formalin-fixed paraffin-embedded human pancreas stained with mitochondria monoclonal antibody (113-1). Image using the Azide and BSA Free form of this antibody.
Mitochondria Antibody (113-1)

Immunocytochemistry/Immunofluorescence: Mouse Monoclonal Mitochondria Antibody (113-1) [NBP2-32980]

Immunocytochemistry/Immunofluorescence: Mouse Monoclonal Mitochondria Antibody (113-1) [NBP2-32980] - NBP2-32980 (green) stains mitochondria in human primary astrocytes. Cells have been counterstained with Hoechst (blue). Image from a verified customer review.

Applications for Mitochondria Antibody (113-1)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Frozen

0.5-1.0ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Reviewed Applications

Read 1 review rated 5 using NBP2-32980 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Mitochondria

NB600-556 recognizes a 60kDa non-glycosylated protein component of mitochondria found in human cells. It can be used to stain the mitochondria in cell or tissue preparations and can be used as a marker of the mitochondria in subcellular fractions. This monoclonal antibody is part of a new panel of reagents which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb-2 produces a spaghetti-like staining pattern in the cytoplasm of normal and malignant human cells and may be used to stain the mitochondria in paraformaldehyde fixed frozen and formalin-fixed, paraffin-embedded tissues.

Additional Mitochondria Products

Product Documents for Mitochondria Antibody (113-1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Mitochondria Antibody (113-1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Mitochondria Antibody (113-1)

Customer Reviews for Mitochondria Antibody (113-1) (1)

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  • Mitochondria Antibody (113-1)
    Name: Pauline Jeannot
    Application: Immunofluorescence
    Sample Tested: primary astrocytes
    Species: Human
    Verified Customer | Posted 08/21/2024
    NBP2-32980 (green) stains mitochondria in human primary astrocytes. Cells have been counterstained with Hoechst (blue)
    Mitochondria Antibody (113-1) NBP2-32980

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