Mitofilin Antibody - BSA Free

Novus Biologicals | Catalog # NB100-1919

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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Feline, Primate

Cited:

Human, Mouse, Primate

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Mitofilin Primary Antibodies »

Product Specifications

Immunogen

Whole human recombinant Mitofilin [UniProt# Q16891]

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:33761951). Rat reactivity reported in scientific literature (PMID: 26729625)

Localization

Mitochondria

Marker

Mitochondria Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Mitofilin Antibody - BSA Free

Western Blot: Mitofilin Antibody [NB100-1919]

Western Blot: Mitofilin Antibody [NB100-1919]

Western Blot: Mitofilin Antibody [NB100-1919] - Western blot of dog testis. Total protein extract 1:2000 dilution of antibody.
Immunocytochemistry/ Immunofluorescence: Mitofilin Antibody [NB100-1919]

Immunocytochemistry/ Immunofluorescence: Mitofilin Antibody [NB100-1919]

Immunocytochemistry/Immunofluorescence: Mitofilin Antibody [NB100-1919] - HeLa cells with antibody 1:500 dilution labeling mitochondria.
Immunohistochemistry-Paraffin: Mitofilin Antibody [NB100-1919]

Immunohistochemistry-Paraffin: Mitofilin Antibody [NB100-1919]

Immunohistochemistry-Paraffin: Mitofilin Antibody [NB100-1919] - IHC-P detection of Mitofilin/Mitochondrial inner membrane protein in a section of normal human skin using Mitofilin antibody at 1:200 dilution. The representative image shows a specific and expected granular staining of Mitofilin in the keratinocytes as well as the other cells of epidermal layer with no staining in basal cells. [60X Magnification]
Western Blot: Mitofilin Antibody [NB100-1919]

Western Blot: Mitofilin Antibody [NB100-1919]

Mitofilin-Antibody-Western-Blot-NB100-1919-img0012.jpg
Immunohistochemistry-Paraffin: Mitofilin Antibody [NB100-1919]

Immunohistochemistry-Paraffin: Mitofilin Antibody [NB100-1919]

Immunohistochemistry-Paraffin: Mitofilin Antibody [NB100-1919] - IHC-P analysis of Mitofilin/Mitochondrial inner membrane protein in a transverse section of normal human skeletal muscle using Mitofilin antibody at 1:200 dilution. The myocytes showed an expected and very specific granular staining in the cytoplasm. [10X Magnification]
Immunohistochemistry-Paraffin: Mitofilin Antibody [NB100-1919]

Immunohistochemistry-Paraffin: Mitofilin Antibody [NB100-1919]

Immunohistochemistry-Paraffin: Mitofilin Antibody [NB100-1919] - IHC-P detection of Mitofilin/Mitochondrial inner membrane protein in a section of normal human skin using Mitofilin antibody at 1:200 dilution. Specific cytoplasmic positivity of Mitofilin was observed in the keratinocytes as well as other cells of the epidermal layer except for basal cells and dermal connective tissue. [10X Magnification]
Mitofilin Antibody

Western Blot: Mitofilin Antibody [NB100-1919] -

Western Blot: Mitofilin Antibody [NB100-1919] - COA7 pathological variants are import‐defectiveASchematic representation of wild‐type & mutant COA7.BCellular protein extracts were isolated from HEK293 cells that were transfected with a plasmid that encoded wild‐type or mutant COA7. The samples were analyzed by reducing SDS–PAGE & Western blot.CCellular fractions were prepared from HEK293 cells that were transfected with a plasmid that encoded wild‐type or mutant COA7. The fractions were analyzed by reducing SDS–PAGE & Western blot. T, total; C, cytosol; M, mitochondria.DHEK293 cells that transiently expressed wild‐type or mutant COA7HIS were solubilized, & the affinity purification of COA7HIS was performed. The samples were analyzed by reducing & non‐reducing SDS–PAGE & Western blot. Load: 3%. Eluate: 100%.EEqual amounts of wild‐type & mutant radiolabeled [35S]COA7 precursors were imported into mitochondria isolated from HEK293 cells. The samples were analyzed by reducing SDS–PAGE & autoradiography. The results of three biological replicates were analyzed, quantified, & normalized to wild‐type COA7 at 45 min. The data are expressed as a mean ± SEM (n = 3). IAA, iodoacetamide.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30885959), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Mitofilin Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500

Immunohistochemistry

1:200-1:500

Immunohistochemistry-Paraffin

1:200-1:500

Western Blot

1:2000
Application Notes
This Mitofilin antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot, where two bands can be seen at approximately 87-90 kDa.

Reviewed Applications

Read 3 reviews rated 4.3 using NB100-1919 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Mitofilin

Mitofilin is one of the most abundant mitochondrial proteins in the human heart. It appears to play an important role in the maintenance of cristae morphology. Mitofilin was originally described as heart muscle protein (HMP) because of its high expression in the heart. Mitochondria are the center of cellular energy production and essential metabolic reactions.

Alternate Names

DKFZp779P1653, inner membrane protein, mitochondrial, MGC111146, Mic 60, Mic60, MICOS Complex Subunit MIC60, mitochondrial (mitofilin), Mitofilin, motor protein, p87/89, proliferation-inducing gene 4

Entrez Gene IDs

10989 (Human); 76614 (Mouse); 312444 (Rat)

Gene Symbol

IMMT

UniProt

Q16891 (Human)

Additional Mitofilin Products

Product Documents for Mitofilin Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Mitofilin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Mitofilin Antibody - BSA Free

Customer Reviews for Mitofilin Antibody - BSA Free (3)

4.3 out of 5
3 Customer Ratings
5 Stars
33%
4 Stars
67%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Mitofilin Antibody
    Name: Julian Wong
    Application: Simple Western
    Sample Tested: cell line, whole cell lysate
    Species: Rat
    Verified Customer | Posted 08/18/2017
    B103 whole-cell lysates (concentrations indicated), probed with 1:25 dilution of antibody on the standard separation matrix (12-230 kDa).
    Mitofilin Antibody - BSA Free NB100-1919
  • Name: Kathleen Emanuel
    Application: Western Blot
    Sample Tested: Protein lysate from mitochondria isolated from SH-SY5Y cells
    Species: Human
    Verified Customer | Posted 06/14/2016
    Neuroblastoma SH-SY5Y mitochondrial protein lysate probed for mitofillin: Each lane run at 12ug of protein per lane
    Mitofilin Antibody - BSA Free NB100-1919
  • Name: Antigoni Diokmetzidou
    Application: Immunoprecipitation
    Sample Tested: Mouse heart
    Species: Mouse
    Verified Customer | Posted 08/27/2013
    Mitofilin Antibody - BSA Free NB100-1919

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Protocols

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FAQs for Mitofilin Antibody - BSA Free

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  • Q: One of our customers is interested in testing your Anti-Mitofilin antibody (NB100-1919) with IHC. Do you know how likely this Ab will be in succeeding to this attempt?

    A: This Ab has been validated for use in IHC-P in Canine, Feline, Mouse, Rat and Human.

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