Mitofusin 2 Antibody (4H8) - Azide and BSA Free
Novus Biologicals | Catalog # H00009927-M03
![Western Blot: Mitofusin 2 Antibody (4H8) [H00009927-M03]](https://resources.rndsystems.com/images/products/Mitofusin-2-Antibody-4H8-Western-Blot-H00009927-M03-img0009.jpg "Western Blot: Mitofusin 2 Antibody (4H8) [H00009927-M03]")
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Key Product Details
Species Reactivity
Human, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, RNA Inhibition
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 4H8
Format
Azide and BSA Free
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Product Specifications
Immunogen
MFN2 (NP_055689, 661 a.a. ~ 757 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. FKRQFVEHASEKLQLVISYTGSNCSHQVQQELSGTFAHLCQQVDVTRENLEQEIAAMNKKIEVLDSLQSKAKLLRNKAGWLDSELNMFTHQYLQPSR
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Scientific Data Images for Mitofusin 2 Antibody (4H8) - Azide and BSA Free
Western Blot: Mitofusin 2 Antibody (4H8) [H00009927-M03]
Western Blot: Mitofusin 2 Antibody (4H8) [H00009927-M03] - Analysis of MFN2 expression in PC-12 (Cat # L012V1).![Immunohistochemistry-Paraffin: Mitofusin 2 Antibody (4H8) [H00009927-M03]](https://resources.rndsystems.com/images/products/Mitofusin-2-Antibody-4H8-Immunohistochemistry-Paraffin-H00009927-M03-img0007.jpg "Immunohistochemistry-Paraffin: Mitofusin 2 Antibody (4H8) [H00009927-M03]")
Immunohistochemistry-Paraffin: Mitofusin 2 Antibody (4H8) [H00009927-M03]
Immunohistochemistry-Paraffin: Mitofusin 2 Antibody (4H8) [H00009927-M03] - Analysis of monoclonal antibody to MFN2 on formalin-fixed paraffin-embedded human heart. Antibody concentration 5 ug/ml![Western Blot: Mitofusin 2 Antibody (4H8) [H00009927-M03]](https://resources.rndsystems.com/images/products/Mitofusin-2-Antibody-4H8-Western-Blot-H00009927-M03-img0004.jpg "Western Blot: Mitofusin 2 Antibody (4H8) [H00009927-M03]")
Western Blot: Mitofusin 2 Antibody (4H8) [H00009927-M03]
Western Blot: Mitofusin 2 Antibody (4H8) [H00009927-M03] - Analysis of MFN2 over-expressed 293 cell line, cotransfected with MFN2 Validated Chimera RNAi ( Cat # H00009927-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MFN2 monoclonal antibody (M03), clone 4H8 (Cat # H00009927-M03 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.![Western Blot: Mitofusin 2 Antibody (4H8) [H00009927-M03]](https://resources.rndsystems.com/images/products/Mitofusin-2-Antibody-4H8-Western-Blot-H00009927-M03-img0008.jpg "Western Blot: Mitofusin 2 Antibody (4H8) [H00009927-M03]")
Western Blot: Mitofusin 2 Antibody (4H8) [H00009927-M03]
Western Blot: Mitofusin 2 Antibody (4H8) [H00009927-M03] - Analysis of MFN2 expression in HeLa (Cat # L013V1).![Western Blot: Mitofusin 2 Antibody (4H8) [H00009927-M03]](https://resources.rndsystems.com/images/products/Mitofusin-2-Antibody-4H8-Western-Blot-H00009927-M03-img0010.jpg "Western Blot: Mitofusin 2 Antibody (4H8) [H00009927-M03]")
Western Blot: Mitofusin 2 Antibody (4H8) [H00009927-M03]
Western Blot: Mitofusin 2 Antibody (4H8) [H00009927-M03] - Analysis of MFN2 expression in transfected 293T cell line by MFN2 monoclonal antibody (M03), clone 4H8. Lane 1: MFN2 transfected lysatE (86.4 KDa). Lane 2: Non-transfected lysate.![ELISA: Mitofusin 2 Antibody (4H8) [H00009927-M03]](https://resources.rndsystems.com/images/products/Mitofusin-2-Antibody-4H8-ELISA-H00009927-M03-img0011.jpg "ELISA: Mitofusin 2 Antibody (4H8) [H00009927-M03]")
ELISA: Mitofusin 2 Antibody (4H8) [H00009927-M03]
ELISA: Mitofusin 2 Antibody (4H8) [H00009927-M03] - Detection limit for recombinant GST tagged MFN2 is approximately 1ng/ml as a capture antibody.Applications for Mitofusin 2 Antibody (4H8) - Azide and BSA Free
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
RNA Inhibition
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 4 using H00009927-M03 in the following applications:
Formulation, Preparation, and Storage
Purification
Ascites
Formulation
In 1x PBS, pH 7.4
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Mitofusin 2
Alternate Names
CMT2A, CMT2A2, CPRP1, HSG, MARF, MFN2
Gene Symbol
MFN2
Additional Mitofusin 2 Products
Product Documents for Mitofusin 2 Antibody (4H8) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Mitofusin 2 Antibody (4H8) - Azide and BSA Free
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for Mitofusin 2 Antibody (4H8) - Azide and BSA Free
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Customer Reviews for Mitofusin 2 Antibody (4H8) - Azide and BSA Free (1)
4 out of 5
1 Customer Rating
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Application: Simple WesternSample Tested: cell line, whole cell lysateSpecies: RatVerified Customer | Posted 08/18/2017B103 whole-cell lysates (concentrations indicated), probed with 1:25 dilution of antibody on the standard separation matrix (12-230 kDa).
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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