MKP-1/DUSP1 Antibody

Novus Biologicals | Catalog # NB100-834

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Predicted:

Bovine (100%), Canine (100%), Mouse (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all MKP-1 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence SYLQSPITTSPSC corresponding to C-Terminus according to NP_004408.1.

Reactivity Notes

Rat reactivity reported from a verified customer review.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for MKP-1/DUSP1 Antibody

Western Blot: MKP-1/DUSP1 Antibody [NB100-834]

Western Blot: MKP-1/DUSP1 Antibody [NB100-834]

Western Blot: MKP-1/DUSP1 Antibody [NB100-834] - Staining of HeLa cell lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: MKP-1/DUSP1 Antibody [NB100-834]

Immunohistochemistry-Paraffin: MKP-1/DUSP1 Antibody [NB100-834]

Immunohistochemistry-Paraffin: MKP-1/DUSP1 Antibody [NB100-834] - Negative Control showing staining of paraffin embedded Human Prostate, with no primary antibody.
Immunohistochemistry-Paraffin: MKP-1/DUSP1 Antibody [NB100-834]

Immunohistochemistry-Paraffin: MKP-1/DUSP1 Antibody [NB100-834]

Immunohistochemistry-Paraffin: MKP-1/DUSP1 Antibody [NB100-834] - Staining of paraffin embedded Human Prostate. Antibody at 8 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Applications for MKP-1/DUSP1 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

8 ug/mL

Peptide ELISA

Detection limit 1:64000

Western Blot

1 - 3 ug/mL
Application Notes
WB: Approx. 40 kDa band observed in human lysates of HeLa (calculated MW of 39.3 kDa band according to NP_004408.1). Customers have reported positive results on mouse microglia (BV2 cells), rat primary microglia, and rat spinal cord homogenate.

Reviewed Applications

Read 1 review rated 4 using NB100-834 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MKP-1

The expression of DUSP1 gene is induced in human skin fibroblasts by oxidative/heat stress and growth factors. It specifies a protein with structural features similar to members of the non-receptor-type protein-tyrosine phosphatase family, and which has significant amino-acid sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus. The bacterially expressed and purified DUSP1 protein has intrinsic phosphatase activity, and specifically inactivates mitogen-activated protein (MAP) kinase in vitro by the concomitant dephosphorylation of both its phosphothreonine and phosphotyrosine residues. Furthermore, it suppresses the activation of MAP kinase by oncogenic ras in extracts of Xenopus oocytes. Thus, DUSP1 may play an important role in the human cellular response to environmental stress as well as in the negative regulation of cellular proliferation.

Long Name

MAP Kinase Phosphatase 1

Alternate Names

CL100, DUSP1, HVH1, MKP1, PTPN10

Entrez Gene IDs

1843 (Human); 19252 (Mouse)

Gene Symbol

DUSP1

UniProt

NP_004408. (Human)

Additional MKP-1 Products

Product Documents for MKP-1/DUSP1 Antibody

Certificate of Analysis

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Product Specific Notices for MKP-1/DUSP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for MKP-1/DUSP1 Antibody (1)

4 out of 5
1 Customer Rating
5 Stars
0%
4 Stars
100%
3 Stars
0%
2 Stars
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1 Stars
0%

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Customer Images

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  • MKP-1/DUSP1 Antibody
    Name: Alexander Vang
    Application: Western Blot
    Sample Tested: Heart lysates
    Species: Rat
    Verified Customer | Posted 03/19/2018
    Probing MKP-1 with LV Heart tissue from Rats
    Proteins were separated on SDS-PAGE and transferred onto PVDF membrane. Membrane was blocked with 5% milk in TBS 0.01% tween and then incubated with primary antibody overnight 1:1000. Secondary was used at 1:5000 and then membrane was ECL and imaged with GE Imagequant imager.
    MKP-1/DUSP1 Antibody NB100-834

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Protocols

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FAQs

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