MKRN2 Antibody (5F8) - Azide and BSA Free

Novus Biologicals | Catalog # H00023609-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 5F8

Format

Azide and BSA Free
View all MKRN2 Primary Antibodies »

Product Specifications

Immunogen

MKRN2 (NP_054879, 317 a.a. ~ 415 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. AFKQGMGKKACKYFEQGKGTCPFGSKCLYRHAYPDGRLAEPEKPRKQLSSQGTVRFFNSVRLWDFIENRESRHVPNNEDVDMTELGDLFMHLSGVESSE

Reactivity Notes

Human. Other species not tested.

Specificity

MKRN2 - makorin, ring finger protein, 2

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for MKRN2 Antibody (5F8) - Azide and BSA Free

Western Blot: MKRN2 Antibody (5F8) [H00023609-M01]

Western Blot: MKRN2 Antibody (5F8) [H00023609-M01]

Western Blot: MKRN2 Antibody (5F8) [H00023609-M01] - MKRN2 monoclonal antibody (M01), clone 5F8 Analysis of MKRN2 expression in Hela S3 NE.
Immunocytochemistry/ Immunofluorescence: MKRN2 Antibody (5F8) [H00023609-M01]

Immunocytochemistry/ Immunofluorescence: MKRN2 Antibody (5F8) [H00023609-M01]

Immunocytochemistry/Immunofluorescence: MKRN2 Antibody (5F8) [H00023609-M01] - Analysis of monoclonal antibody to MKRN2 on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: MKRN2 Antibody (5F8) [H00023609-M01]

Western Blot: MKRN2 Antibody (5F8) [H00023609-M01]

Western Blot: MKRN2 Antibody (5F8) [H00023609-M01] - Analysis of MKRN2 over-expressed 293 cell line, cotransfected with MKRN2 Validated Chimera RNAi ( Cat # H00023609-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MKRN2 monoclonal antibody (M01), clone 5F8 (Cat # H00023609-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot: MKRN2 Antibody (5F8) [H00023609-M01]

Western Blot: MKRN2 Antibody (5F8) [H00023609-M01]

Western Blot: MKRN2 Antibody (5F8) [H00023609-M01] - Analysis of MKRN2 expression in transfected 293T cell line by MKRN2 monoclonal antibody (M01), clone 5F8.Lane 1: MKRN2 transfected lysate(46.9 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: MKRN2 Antibody (5F8) [H00023609-M01] - Detection limit for recombinant GST tagged MKRN2 is approximately 0.1ng/ml as a capture antibody.

Applications for MKRN2 Antibody (5F8) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, RNAi validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MKRN2

Members of the makorin family, including MKRN2, have a characteristic zinc finger composition that suggests that they are ribonucleoproteins (Gray et al., 2001 [PubMed 11597136]).[supplied by OMIM]

Alternate Names

EC 6.3.2.-, HSPC070, makorin ring finger protein 2, probable E3 ubiquitin-protein ligase makorin-2, RNF62RING finger protein 62

Entrez Gene IDs

23609 (Human)

Gene Symbol

MKRN2

OMIM

608426 (Human)

UniProt

NP_054879 (Human)

Additional MKRN2 Products

Product Documents for MKRN2 Antibody (5F8) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for MKRN2 Antibody (5F8) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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