MKRN2 Antibody

Western Blot: MKRN2 Antibody [NBP2-17301]

Western Blot: MKRN2 Antibody [NBP2-17301] - Sample (30 ug of whole cell lysate) A: Jurkat 10% SDS PAGE gel, diluted at 1:1000.

Immunohistochemistry-Paraffin: MKRN2 Antibody [NBP2-17301]

Immunohistochemistry-Paraffin: MKRN2 Antibody [NBP2-17301] - U373 xenograft, using MKRN2 antibody at 1:500 dilution.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: MKRN2 Antibody [NBP2-17301] -

Investigation of the phenotypic effect of MKRN2 depletion on IAV replication.(A) Demonstration by Western blotting that the MKRN2 siRNA pair reduce MKRN2 protein levels at 48 h post transfection. Knockdown of MKRN2 in A549 cells, followed by infection with A/WSN/33 (B), A/California/7/2009 (C) or A/Norway/466/2014 (D) significantly reduced virus output from these cells as measured by plaque assay. (E) Western blot demonstrating that the MKRN2 siRNA pair reduce expression of endogenous and lentiviral expressed MKRN2 in an overexpression A549 cell line, while an siRNA pair targeting the MKRN2 3’ UTR only reduce expression of the endogenous MKRN2 protein. (F) A/California/7/2009 titres from MKRN2 overexpression A549 cells demonstrate that while reducing all MKRN2 expression significantly reduced virus output, knocking down endogenous MKRN2 while expressing wild-type MKRN2 from a lentiviral cassette rescues this phenotype. (G) Early viral RNA dynamics in siMKRN2 treated cells were quantified by qPCR, revealing that a loss of MKRN2 significantly reduced viral NP mRNA levels early in infection. (H) Overall titres from low MOI infections (MOI 0.01) were measured at 6, 24 and 48 hpi for both conditions, (I) alongside NP mRNA, vRNA and MKRN2 mRNA levels at matching timepoints. (J) High MOI infections (MOI 3) were performed in similarly siRNA treated cells and total RNA was harvested at 0, 2, 4 and 6 hpi. Again NP mRNA, vRNA and MKRN2 mRNA levels were quantified in these sample, representing single round infection dynamics. Image collected and cropped by CiteAb from the following open publication (https://dx.plos.org/10.1371/journal.ppat.1012231), licensed under a CC-BY license. Not internally tested by Novus Biologicals.