MLKL Antibody (3B2) - Azide and BSA Free
Novus Biologicals | Catalog # H00197259-M02
![Immunocytochemistry/ Immunofluorescence: MLKL Antibody (3B2) [H00197259-M02]](https://beta-resources.rndsystems.com/images/products/MLKL-Antibody-3B2-Immunocytochemistry-Immunofluorescence-H00197259-M02-img0005.jpg "Immunocytochemistry/ Immunofluorescence: MLKL Antibody (3B2) [H00197259-M02]")
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Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Cited:
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 3B2
Format
Azide and BSA Free
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Product Specifications
Immunogen
MLKL (NP_689862, 371 a.a. ~ 471 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.VKSTAYLSPQELEDVFYQYDVKSEIYSFGIVLWEIATGDIPFQGCNSEKIRKLVAVKRQQEPLGEDCPSELREIIDECRAHDPSVRPSVDEILKKLSTFSK
Specificity
MLKL - mixed lineage kinase domain-like (3B2)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Novus Biologicals Mouse MLKL Antibody (3B2) - Azide and BSA Free (H00197259-M02) is a monoclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-MLKL Antibody: Cited in 4 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for MLKL Antibody (3B2) - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence: MLKL Antibody (3B2) [H00197259-M02]
Immunocytochemistry/Immunofluorescence: MLKL Antibody (3B2) [H00197259-M02] - Analysis of monoclonal antibody to MLKL on HeLa cell. Antibody concentration 10 ug/ml.![Immunohistochemistry-Paraffin: MLKL Antibody (3B2) [H00197259-M02]](https://beta-resources.rndsystems.com/images/products/MLKL-Antibody-3B2-Immunohistochemistry-Paraffin-H00197259-M02-img0006.jpg "Immunohistochemistry-Paraffin: MLKL Antibody (3B2) [H00197259-M02]")
Immunohistochemistry-Paraffin: MLKL Antibody (3B2) [H00197259-M02]
Immunohistochemistry-Paraffin: MLKL Antibody (3B2) [H00197259-M02] - Analysis of monoclonal antibody to MLKL on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.![ELISA: MLKL Antibody (3B2) [H00197259-M02]](https://beta-resources.rndsystems.com/images/products/MLKL-Antibody-3B2-ELISA-H00197259-M02-img0008.jpg "ELISA: MLKL Antibody (3B2) [H00197259-M02]")
ELISA: MLKL Antibody (3B2) [H00197259-M02]
ELISA: MLKL Antibody (3B2) [H00197259-M02] - Detection limit for recombinant GST tagged MLKL is 0.3 ng/ml as a capture antibody.Applications for MLKL Antibody (3B2) - Azide and BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Western Blot
1:500
Application Notes
Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: MLKL
Long Name
Mixed Lineage Kinase Domain-like
Alternate Names
FLJ34389, mixed lineage kinase domain-like, mixed lineage kinase domain-like protein
Entrez Gene IDs
197259 (Human)
Gene Symbol
MLKL
UniProt
Additional MLKL Products
Product Documents for MLKL Antibody (3B2) - Azide and BSA Free
Product Specific Notices for MLKL Antibody (3B2) - Azide and BSA Free
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for MLKL Antibody (3B2) - Azide and BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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