MLKL [p Ser345] Antibody (JM92-37)

MLKL [p Ser345] Antibody (JM92-37) [NBP2-66953] -

Immunohistochemical analysis of paraffin-embedded mouse lung tissue using MLKL [p Ser345] Antibody (JM92-37). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

MLKL [p Ser345] Antibody (JM92-37) [NBP2-66953] -

Western blot analysis of MLKL [p Ser345] on different lysates with MLKL [p Ser345] Antibody (JM92-37) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution.Lane 1: L-929 cell lysateLane 2: L929 treated with 20μM Z-VAD for 3.5 hours, then added 100nM SM-164 and 20ng/ml TNF-alpha for 3 hours cell lysateLysates/proteins at 20 µg/Lane.Predicted band size: 54 kDaObserved band size: 54 kDaExposure time: 1 minute 10 seconds;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

IHC-Frozen:MLKL [p Ser345] Antibody (JM92-37)-

IHC-Frozen:MLKL [p Ser345] Antibody (JM92-37)-Analysis of frozen mouse spleen tissue with Rabbit anti-Phospho-MLKL (S345) antibody at 1/100 dilution.The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Western Blot: MLKL [p Ser345] Antibody (JM92-37) [NBP2-66953]

Western blot analysis on different lysates with NBP2-66953 at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution. Lane 1: L-929 cell lysate Lane 2: L929 treated with 20μM Z-VAD for 3.5 hours, then added 100nM SM-164 and 20ng/ml TNF-alpha for 3 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 1 minute 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-66953 at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.