MLLT1 Antibody (3H2) - Azide and BSA Free

Novus Biologicals | Catalog # H00004298-M01

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3H2

Format

Azide and BSA Free
View all MLLT1 Primary Antibodies »

Product Specifications

Immunogen

MLLT1 (NP_005925, 472 a.a. ~ 558 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GRRSPESCSKPEKILKKGTYDKAYTDELVELHRRLMALRERNVLQQIVNLIEETGHFNVTNTTFDFDLFSLDETTVRKLQSCLEAVA

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25481096). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

MLLT1 - myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for MLLT1 Antibody (3H2) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: MLLT1 Antibody (3H2) [H00004298-M01]

Immunocytochemistry/ Immunofluorescence: MLLT1 Antibody (3H2) [H00004298-M01]

Immunocytochemistry/Immunofluorescence: MLLT1 Antibody (3H2) [H00004298-M01] - Analysis of monoclonal antibody to MLLT1 on HeLa cell. Antibody concentration 10 ug/ml.
Sandwich ELISA: MLLT1 Antibody (3H2) [H00004298-M01] - Detection limit for recombinant GST tagged MLLT1 is approximately 0.1ng/ml as a capture antibody.
MLLT1 Antibody (3H2)

Western Blot: Mouse Monoclonal MLLT1 Antibody (3H2) [H00004298-M01]

Western Blot: Mouse Monoclonal MLLT1 Antibody (3H2) [H00004298-M01] - Western Blot showing amount of protein, cell lines tested (293T, over expression line 293T+hENL, and mESCs) and loading control blot using alpha-tubulin. Image from a verified customer review.

Applications for MLLT1 Antibody (3H2) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Sandwich ELISA

1:100-1:2000

Western Blot

1:500
Application Notes
Antibody reactivity against recombinant protein with GST tag on ELISA and WB. GST tag alone is used as a negative control. It has been used for IF. IHC-P usage reported in scientific literature (PMID: 25481096).

Reviewed Applications

Read 1 review rated 3 using H00004298-M01 in the following applications:

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MLLT1

MLLT1, also known as ENL (eleven nineteen leukemia protein), was originally identified as a gene involved in a recurrent translocation (11;19) with the mixed-lineage leukemia (MLL) gene. The MLL-ENL fusion protein has been found to associate with SWI/SNF complexes and promote transcription from the HoxA7 promoter. Additionally, from studies to identify proteins that associate with MLLT1, MLLT1 is suggested to play a role in histone modification and transcriptional elongation.

Alternate Names

ENLENL/MLL fusion, LTG19MLLT1/MLL fusion, myeloid/lymphoid or mixed-lineage leukemia (trithorax (Drosophila) homolog);translocated to, 1, myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila);translocated to, 1, protein ENL, YEATS domain-containing protein 1, YEATS1myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog); translocatedto, 1

Entrez Gene IDs

4298 (Human)

Gene Symbol

MLLT1

OMIM

159556 (Human)

UniProt

NP_005925 (Human)

Additional MLLT1 Products

Product Documents for MLLT1 Antibody (3H2) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for MLLT1 Antibody (3H2) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for MLLT1 Antibody (3H2) - Azide and BSA Free

Customer Reviews for MLLT1 Antibody (3H2) - Azide and BSA Free (1)

3 out of 5
1 Customer Rating
5 Stars
0%
4 Stars
0%
3 Stars
100%
2 Stars
0%
1 Stars
0%

Have you used MLLT1 Antibody (3H2) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • Great for human MLLT1 but not the best for mouse MLLT1
    Name: Coral Wille
    Application: Western Blot
    Sample Tested: mESCs and HEK293 human embryonic kidney cell line
    Species: Human and Mouse
    Verified Customer | Posted 07/01/2024
    Annnotated WB image showing amount of protein, cell lines tested (293T, over expression line 293T+hENL, and mESCs), loading control blot using alpha-tubulin
    Western blot with 30µg of protein per sample, antibody at a 1:500 dilution. Used wild type HEK293T cells and HEK293Ts with over expression of hENL. We also used mouse embryonic stem cells. Used alpha-tubulin as a loading control for each well.
    MLLT1 Antibody (3H2) - Azide and BSA Free H00004298-M01

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for MLLT1 Antibody (3H2) - Azide and BSA Free

Showing  1 - 1 of 1 FAQ Showing All

  • Q: I'm attempting to work out conditions for IF using this antibody (H00004298-M01). However, I'm having a hard time finding publications that have published using this specific antibody and the spec sheet does not outline conditions very well. Any and all help is appreciated.

    A:

    This antibody happens to be one that we distribute for a Taiwanese company called Abnova. As such all of the testing is performed in their labs, please see their protocol (http://www.abnova.com/protocol_pdf/Immunofluorescence.pdf). Additionally, this antibody was used at 10ug/mL on HeLa cells.

Showing  1 - 1 of 1 FAQ Showing All
View all FAQs for Antibodies