MMP-12 Antibody (SR03-23)

Novus Biologicals | Catalog # NBP2-67344

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR03-23 expressed in HEK293
View all MMP-12 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human MMP-12 aa 421-470 / 470. (SwissProt: P39900 Human; SwissProt: P34960 Mouse; SwissProt: Q63341 Rat)

Localization

Secreted.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MMP-12 Antibody (SR03-23)

Western Blot: MMP-12 Antibody (SR03-23) [NBP2-67344]

Western Blot: MMP-12 Antibody (SR03-23) [NBP2-67344]

Western Blot: MMP-12 Antibody (SR03-23) [NBP2-67344] - Analysis of MMP-12 on different lysates with Rabbit anti-MMP-12 antibody at 1/500 dilution. Lane 1: Mouse lung tissue lysate Lane 2: Rat lung tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 54 kDa Observed band size: 40 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.
Immunohistochemistry-Paraffin: MMP-12 Antibody (SR03-23) [NBP2-67344]

Immunohistochemistry-Paraffin: MMP-12 Antibody (SR03-23) [NBP2-67344]

Immunohistochemistry-Paraffin: MMP-12 Antibody (SR03-23) [NBP2-67344] - Analysis of paraffin-embedded mouse lung tissue using anti-MMP12 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Flow Cytometry: MMP-12 Antibody (SR03-23) [NBP2-67344]

Flow Cytometry: MMP-12 Antibody (SR03-23) [NBP2-67344]

Flow Cytometry: MMP-12 Antibody (SR03-23) [NBP2-67344] - Analysis of A549 cells with MMP12 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western Blot: MMP-12 Antibody (SR03-23) [NBP2-67344]

Western Blot: MMP-12 Antibody (SR03-23) [NBP2-67344]

Western Blot: MMP-12 Antibody (SR03-23) [NBP2-67344] - Analysis of MMP12 on different cell lysates using anti-MMP12 antibody at 1/1,000 dilution. Positive control: Lane 1: A549 Lane 2: Hela Lane 3: MCF-7 Lane 4: THP-1
Immunohistochemistry-Paraffin: MMP-12 Antibody (SR03-23) [NBP2-67344]

Immunohistochemistry-Paraffin: MMP-12 Antibody (SR03-23) [NBP2-67344]

Immunohistochemistry-Paraffin: MMP-12 Antibody (SR03-23) [NBP2-67344] - Analysis of paraffin-embedded human breast carcinomna tissue using anti-MMP12 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: MMP-12 Antibody (SR03-23) [NBP2-67344]

Immunohistochemistry-Paraffin: MMP-12 Antibody (SR03-23) [NBP2-67344]

Immunohistochemistry-Paraffin: MMP-12 Antibody (SR03-23) [NBP2-67344] - Analysis of paraffin-embedded mouse lung tissue using anti-MMP12 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: MMP-12 Antibody (SR03-23) [NBP2-67344]

Immunohistochemistry-Paraffin: MMP-12 Antibody (SR03-23) [NBP2-67344]

Immunohistochemistry-Paraffin: MMP-12 Antibody (SR03-23) [NBP2-67344] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-MMP-12 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
MMP-12 Antibody (SR03-23)

Immunohistochemistry-Paraffin: Rabbit Monoclonal MMP-12 Antibody (SR03-23) [NBP2-67344]

Immunohistochemistry-Paraffin: Rabbit Monoclonal MMP-12 Antibody (SR03-23) [NBP2-67344] - Analysis of MMP-12 antibody on human breast cancer tissue. Pretreatment: ER2 100C 20min, Blocking: 2.5% Normal horse serum 10min, Primary Ab: 1/200 60min, HRP for Rabbit 30min, DAB 5min, Hematoxylin counterstain. Image from a verified customer review.

Applications for MMP-12 Antibody (SR03-23)

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunocytochemistry/ Immunofluorescence

1:20-1:50

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:500-1:2000
Application Notes
IP: Use at an assay dependent concentration.

Reviewed Applications

Read 1 review rated 4 using NBP2-67344 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MMP-12

Human macrophage elastase (MMP-12) is a member of the family of matrix metalloproteinases (MMPs) that plays, like other members of the family, an important role in inflammatory processes contributing to tissue remodeling and destruction. In particular, a prominent role of MMP-12 in the destruction of elastin in the lung alveolar wall and the pathogenesis of emphysema has been suggested. It is therefore an attractive therapeutic target (1). MMP-12) expressed mainly in alveolar macrophages has been identified in the mouse lung as the main destructive agent associated with cigarette smoking, which gives rise to emphysema, both directly via elastin degradation and indirectly by disturbing the proteinase/antiproteinase balance via inactivation of the alpha1-proteinase inhibitor (alpha1-PI), the antagonist of the leukocyte elastase (2). Because elastin represents a critical component of aortic wall structure and a matrix substrate for metalloelastases, MMP-12 may have a direct and singular role in the pathogenesis of aortic aneurysms (3).

Long Name

Matrix Metalloproteinase 12

Alternate Names

MMP12

Gene Symbol

MMP12

Additional MMP-12 Products

Product Documents for MMP-12 Antibody (SR03-23)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for MMP-12 Antibody (SR03-23)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Metalloproteases and Regulators

Citations for MMP-12 Antibody (SR03-23)

Customer Reviews for MMP-12 Antibody (SR03-23) (1)

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  • MMP-12 Antibody (SR03-23)
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Breast cancer tissue
    Species: Human
    Verified Customer | Posted 09/30/2024
    pretreatment: ER2 100C 20min Blocking: 2.5% Normal horse serum 10min Primary Ab: SR03-23 1/200 60min HRP for Rabbit 30min DAB 5min Hematoxylin counterstain
    MMP-12 Antibody (SR03-23) NBP2-67344

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