MMP-12 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-31225

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MMP-12 Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human MMP12. The exact sequence is proprietary.

Reactivity Notes

Mouse reactivity reported from a verified customer review.

Localization

Secreted, extracellular space, extracellular matrix

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MMP-12 Antibody - BSA Free

Western Blot: MMP-12 Antibody [NBP1-31225]

Western Blot: MMP-12 Antibody [NBP1-31225]

Western Blot: MMP-12 Antibody [NBP1-31225] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with MMP12 antibody [N3C1], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: MMP-12 Antibody [NBP1-31225]

Immunocytochemistry/ Immunofluorescence: MMP-12 Antibody [NBP1-31225]

Immunocytochemistry/Immunofluorescence: MMP-12 Antibody [NBP1-31225] - Analysis of methanol-fixed H1299, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: MMP-12 Antibody [NBP1-31225]

Immunohistochemistry-Paraffin: MMP-12 Antibody [NBP1-31225]

Immunohistochemistry-Paraffin: MMP-12 Antibody [NBP1-31225] - Imaging of Human breast cancer tissue. MMP12 is stained by antibody (green) and nucleus is stained with DAPI (blue). Image submitted by a verified customer review.
Immunohistochemistry-Paraffin: MMP-12 Antibody [NBP1-31225]

Immunohistochemistry-Paraffin: MMP-12 Antibody [NBP1-31225]

Immunohistochemistry-Paraffin: MMP-12 Antibody [NBP1-31225] - DLD1 xenograft, using MMP12 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: MMP-12 Antibody [NBP1-31225]

Immunohistochemistry-Paraffin: MMP-12 Antibody [NBP1-31225]

Immunohistochemistry-Paraffin: MMP-12 Antibody [NBP1-31225] - Human lung adenocarcinoma. MMP12 antibody [N3C1], Internal diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
MMP-12 Antibody - BSA Free

Western Blot: MMP-12 Antibody - BSA Free [NBP1-31225] -

MMP12 expression is essential for DRP1/FOXM1 regulation in HNC cells. (A) The mRNA and protein expression levels of FOXM1 in siDRP1 cells were examined. Quantification of relative FOXM1 expression is shown. (B,C) Western blotting, QPCR and luciferase activity analysis of MMP12 were determined in SAS cells transfected with FOXM1 or vector control in combination with siDRP1 or negative control. Quantification of relative DRP1, Flag‐FOXM1 and MMP12 expressions is shown. (D,E) Western blotting, QPCR and luciferase activity of MMP12 were analyzed in SAS cells transfected with FOXM1 or vector control in combination with Mdivi‐1 treatment. Quantification of relative DRP1, Flag‐FOXM1, and MMP12 expressions is shown. (F) IHC staining patterns of the HNC tumor tissues for DRP1, FOXM1 and MMP12. Scale bar: 100 um. All data presented as mean +/- SD of three independent experiments. Significance calculated using t‐test. * P < 0.05, ** P < 0.01, *** P < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35313071), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
MMP-12 Antibody - BSA Free

Western Blot: MMP-12 Antibody - BSA Free [NBP1-31225] -

MMP12 is one of the targets of DRP1 in HNC cells. (A) Heat‐map showing relative alteration of target genes belonging to EMT molecules using QPCR array analysis of SAS cell transfected with siDRP1 compared with the negative control. Red: upregulation; green: downregulation. (B) QPCR was analyzed to validate the expressions of target genes from (A). (C) Impact of DRP1 knockdown or Mdivi‐1 on MMP12 protein expression were demonstrated in SAS and HSC‐3 cells. Quantification of relative DRP1 and MMP12 expressions are shown. (D,E) Cell growth and motility were evaluated in DRP1‐depleted cells transfected with MMP12 using MTT and Transwell assays. Quantification of relative Flag‐MMP12 expression is shown. (F) Studies from GEPIA and Oncomine datasets present the increase of MMP12 mRNA in HNC samples. (G) A positive correlation between DRP1 mRNA and MMP12 mRNA was found in the Oncomine cohort (Peng database, n = 41; Estilo database, n = 31). All data are presented as mean +/- SD of three independent experiments. Significance was calculated using t‐test. In (D) (cell growth) and (F) (GEPIA), statistical analyses were performed using one‐way ANOVA followed by Tukey’s multiple comparison’s test and Wilcoxon signed‐rank test, respectively. * P < 0.05, ** P < 0.01, *** P < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35313071), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
MMP-12 Antibody - BSA Free

Western Blot: MMP-12 Antibody - BSA Free [NBP1-31225] -

MMP12 expression is essential for DRP1/FOXM1 regulation in HNC cells. (A) The mRNA and protein expression levels of FOXM1 in siDRP1 cells were examined. Quantification of relative FOXM1 expression is shown. (B,C) Western blotting, QPCR and luciferase activity analysis of MMP12 were determined in SAS cells transfected with FOXM1 or vector control in combination with siDRP1 or negative control. Quantification of relative DRP1, Flag‐FOXM1 and MMP12 expressions is shown. (D,E) Western blotting, QPCR and luciferase activity of MMP12 were analyzed in SAS cells transfected with FOXM1 or vector control in combination with Mdivi‐1 treatment. Quantification of relative DRP1, Flag‐FOXM1, and MMP12 expressions is shown. (F) IHC staining patterns of the HNC tumor tissues for DRP1, FOXM1 and MMP12. Scale bar: 100 um. All data presented as mean +/- SD of three independent experiments. Significance calculated using t‐test. * P < 0.05, ** P < 0.01, *** P < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35313071), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
MMP-12 Antibody - BSA Free

Western Blot: MMP-12 Antibody - BSA Free [NBP1-31225] -

MMP12 is one of the targets of DRP1 in HNC cells. (A) Heat‐map showing relative alteration of target genes belonging to EMT molecules using QPCR array analysis of SAS cell transfected with siDRP1 compared with the negative control. Red: upregulation; green: downregulation. (B) QPCR was analyzed to validate the expressions of target genes from (A). (C) Impact of DRP1 knockdown or Mdivi‐1 on MMP12 protein expression were demonstrated in SAS and HSC‐3 cells. Quantification of relative DRP1 and MMP12 expressions are shown. (D,E) Cell growth and motility were evaluated in DRP1‐depleted cells transfected with MMP12 using MTT and Transwell assays. Quantification of relative Flag‐MMP12 expression is shown. (F) Studies from GEPIA and Oncomine datasets present the increase of MMP12 mRNA in HNC samples. (G) A positive correlation between DRP1 mRNA and MMP12 mRNA was found in the Oncomine cohort (Peng database, n = 41; Estilo database, n = 31). All data are presented as mean +/- SD of three independent experiments. Significance was calculated using t‐test. In (D) (cell growth) and (F) (GEPIA), statistical analyses were performed using one‐way ANOVA followed by Tukey’s multiple comparison’s test and Wilcoxon signed‐rank test, respectively. * P < 0.05, ** P < 0.01, *** P < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35313071), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for MMP-12 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Reviewed Applications

Read 2 reviews rated 4 using NBP1-31225 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MMP-12

Human macrophage elastase (MMP-12) is a member of the family of matrix metalloproteinases (MMPs) that plays, like other members of the family, an important role in inflammatory processes contributing to tissue remodeling and destruction. In particular, a prominent role of MMP-12 in the destruction of elastin in the lung alveolar wall and the pathogenesis of emphysema has been suggested. It is therefore an attractive therapeutic target (1). MMP-12) expressed mainly in alveolar macrophages has been identified in the mouse lung as the main destructive agent associated with cigarette smoking, which gives rise to emphysema, both directly via elastin degradation and indirectly by disturbing the proteinase/antiproteinase balance via inactivation of the alpha1-proteinase inhibitor (alpha1-PI), the antagonist of the leukocyte elastase (2). Because elastin represents a critical component of aortic wall structure and a matrix substrate for metalloelastases, MMP-12 may have a direct and singular role in the pathogenesis of aortic aneurysms (3).

Long Name

Matrix Metalloproteinase 12

Alternate Names

MMP12

Gene Symbol

MMP12

Additional MMP-12 Products

Product Documents for MMP-12 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for MMP-12 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Related Research Areas

Metalloproteases and Regulators

Citations for MMP-12 Antibody - BSA Free

Customer Reviews for MMP-12 Antibody - BSA Free (2)

4 out of 5
2 Customer Ratings
5 Stars
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50%
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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • MMP-12 Antibody
    Name: Ilya Ulasov
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Breast cancer tissue
    Species: Human
    Verified Customer | Posted 02/22/2017
    MMP12-green fluorochrome, nucleus-DAPI
    MMP-12 Antibody - BSA Free NBP1-31225
  • Name: Kelsey Stockbridge
    Application: Western Blot
    Sample Tested: Mouse adipose tissue homogenate, Sample Amount: 20ug
    Species: Mouse
    Verified Customer | Posted 06/21/2011
    MMP-12 Antibody - BSA Free NBP1-31225

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Protocols

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FAQs for MMP-12 Antibody - BSA Free

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  • Q: Does this antibody detect the catalytic domain alone of this protein?

    A:

    The immunogen for this protein is between amino acids 213 and 307 of MMP12 (UniProt ID#P39900). This corresponds to a portion of the Hemopexin-like 1 domain on UniProt and does not cover the whole catalytic domain (ZnMc_MMP) which is between amino acids 109 and 263. Please check NCBI Gene ID: 4321 (https://www.ncbi.nlm.nih.gov/gene/?term=4321) for more information.

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