MMP-13 Antibody (OTI2D8) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-72740

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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI2D8

Format

Azide and BSA Free
View all MMP-13 Primary Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 104-471 of human MMP13 (NP_002418) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

42.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MMP-13 Antibody (OTI2D8) - Azide and BSA Free

Western Blot: MMP-13 Antibody (OTI2D8)Azide and BSA Free [NBP2-72740]

Western Blot: MMP-13 Antibody (OTI2D8)Azide and BSA Free [NBP2-72740]

Western Blot: MMP-13 Antibody (OTI2D8) - Azide and BSA Free [NBP2-72740] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MMP-13.
Immunocytochemistry/ Immunofluorescence: MMP-13 Antibody (OTI2D8) - Azide and BSA Free [NBP2-72740]

Immunocytochemistry/ Immunofluorescence: MMP-13 Antibody (OTI2D8) - Azide and BSA Free [NBP2-72740]

Immunocytochemistry/Immunofluorescence: MMP-13 Antibody (OTI2D8) - Azide and BSA Free [NBP2-72740] - Anti-MMP13 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MMP13.
Western Blot: MMP-13 Antibody (OTI2D8)Azide and BSA Free [NBP2-72740]

Western Blot: MMP-13 Antibody (OTI2D8)Azide and BSA Free [NBP2-72740]

Western Blot: MMP-13 Antibody (OTI2D8) - Azide and BSA Free [NBP2-72740] - Analysis of extracts (10ug) from 5 different cell lines by using anti-MMP13 monoclonal antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: MMP-13 Antibody (OTI2D8) - Azide and BSA Free [NBP2-72740]

Immunohistochemistry-Paraffin: MMP-13 Antibody (OTI2D8) - Azide and BSA Free [NBP2-72740]

Immunohistochemistry-Paraffin: MMP-13 Antibody (OTI2D8) - Azide and BSA Free [NBP2-72740] - Human lymph node tissue within the normal limits using anti-MMP13 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120 degrees C for 3min.
Immunohistochemistry: MMP-13 Antibody (OTI2D8) - Azide and BSA Free [NBP2-72740]

Immunohistochemistry: MMP-13 Antibody (OTI2D8) - Azide and BSA Free [NBP2-72740]

Immunohistochemistry: MMP-13 Antibody (OTI2D8) - Azide and BSA Free [NBP2-72740] - Analysis of Carcinoma of Human bladder tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: MMP-13 Antibody (OTI2D8) - Azide and BSA Free [NBP2-72740]

Immunohistochemistry: MMP-13 Antibody (OTI2D8) - Azide and BSA Free [NBP2-72740]

Immunohistochemistry: MMP-13 Antibody (OTI2D8) - Azide and BSA Free [NBP2-72740] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: MMP-13 Antibody (OTI2D8) - Azide and BSA Free [NBP2-72740]

Immunohistochemistry: MMP-13 Antibody (OTI2D8) - Azide and BSA Free [NBP2-72740]

Immunohistochemistry: MMP-13 Antibody (OTI2D8) - Azide and BSA Free [NBP2-72740] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: MMP-13 Antibody (OTI2D8) - Azide and BSA Free [NBP2-72740]

Immunohistochemistry: MMP-13 Antibody (OTI2D8) - Azide and BSA Free [NBP2-72740]

Immunohistochemistry: MMP-13 Antibody (OTI2D8) - Azide and BSA Free [NBP2-72740] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: MMP-13 Antibody (OTI2D8) - Azide and BSA Free [NBP2-72740]

Immunohistochemistry: MMP-13 Antibody (OTI2D8) - Azide and BSA Free [NBP2-72740]

Immunohistochemistry: MMP-13 Antibody (OTI2D8) - Azide and BSA Free [NBP2-72740] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Applications for MMP-13 Antibody (OTI2D8) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:4000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MMP-13

The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-12 (also designated macrophage metalloelastase) is produced in alveolar macrophages and degrades elastin. MMP-12 may contribute to elastin degradation occurring in granulomatous skin diseases and may also participate in macrophage migration through the epidermal and vascular basement membranes in inflammatory disorders.

Long Name

Matrix Metalloproteinase 13

Alternate Names

MMP13

Gene Symbol

MMP13

Additional MMP-13 Products

Product Documents for MMP-13 Antibody (OTI2D8) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for MMP-13 Antibody (OTI2D8) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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