MMP-14/MT1-MMP Antibody (LEM-2/15) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-09002

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Block/Neutralize, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 kappa Clone # LEM-2/15

Format

Azide and BSA Free
View all MMP-14/MT1-MMP Primary Antibodies »

Product Specifications

Immunogen

The cyclic peptide possessing the sequence of residues 218-233 of the V-B-loop of MT1-MMP.

Specificity

This antibody binds to residues 218-233 of the V-B loop of MT1-MMP with a Kd of 2.32E10-9M.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for MMP-14/MT1-MMP Antibody (LEM-2/15) - Azide and BSA Free

Western Blot: MMP-14/MT1-MMP Antibody (LEM-2/15)Azide and BSA Free [NBP3-09002]

Western Blot: MMP-14/MT1-MMP Antibody (LEM-2/15)Azide and BSA Free [NBP3-09002]

Western Blot: MMP-14/MT1-MMP Antibody (LEM-2/15) [NBP3-09002] - A549 (A), HepG2 (B) and HeLa (C) cell lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric mouse IgG1 version of LEM-2/15 (NBP3-09002) at 0.3 ug/ml before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The predicted running size for unprocessed MMP-14/MT1-MMP is 65.9 kDa. A signal peptide and propeptide are cleaved from MMP-14/MT1-MMP during post-translational processing. The processed form of MMP-14/MT1-MMP may be seen in all samples, and the unprocessed form in lanes B and C. NBP3-09002 successfully detected MMP-14/MT1-MMP in A549, HepG2 and HeLa cell lysates.

Applications for MMP-14/MT1-MMP Antibody (LEM-2/15) - Azide and BSA Free

Application
Recommended Usage

Block/Neutralize

Optimal dilutions of this antibody should be experimentally determined.

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody binds to residues 218-233 of the V-B loop of MT1-MMP which is a mediator of cell migration and is frequently involved in malignancy of tumours by promoting metastasis. The antibody acts as a non-competitive inhibitor of the receptor.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MMP-14/MT1-MMP

The matrix metalloproteinase (MMP) family (approximately 25 members in mammals) has been implicated in extracellular matrix remodeling associated with embryonic development, cancer formation and progression, and various other physiological and pathological events. MMP-14 is a membrane-bound matrix metalloproteinase (MT-MMP) capable of mediating pericellular proteolysis of extracellular matrix components. MMP-14 is therefore thought to be an important molecular tool for cellular remodeling of the surrounding matrix (1). Gelatinase A (type-IV collagenase; M(r) 72,000) is produced by tumour stroma cells and is believed to be crucial for their invasion and metastasis, acting by degrading extracellular matrix macro-molecules such as type IV collagen. MMP-14 may thus trigger invasion by tumor cells by activating pro-gelatinase A on the tumor cell surface (2). Observations identify MT1-MMP as a tumor-derived growth factor that regulates proliferation by controlling cell geometry within the confines of the 3D extracellular matrix (ECM) (3).

Long Name

Matrix Metalloproteinase 14/Membrane Type 1 MMP

Alternate Names

MMP-X1, MMP14, MT-MMP1, MT1-MMP

Gene Symbol

MMP14

Additional MMP-14/MT1-MMP Products

Product Documents for MMP-14/MT1-MMP Antibody (LEM-2/15) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for MMP-14/MT1-MMP Antibody (LEM-2/15) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Metalloproteases and Regulators

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Protocols

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