Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin, Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MMP-3 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an C-terminal portion of the human MMP3 protein (between residues 400-477) [UniProt P08254]

Reactivity Notes

Human, Mouse and Rat.

Marker

Mesenchymal Cells Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit MMP-3 Antibody - BSA Free (NB100-91878) is a polyclonal antibody validated for use in IHC and WB. Anti-MMP-3 Antibody: Cited in 11 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for MMP-3 Antibody - BSA Free

Western Blot: MMP-3 AntibodyBSA Free [NB100-91878]

Western Blot: MMP-3 AntibodyBSA Free [NB100-91878]

Western Blot: MMP-3 Antibody [NB100-91878] - Analysis of MMP-3 (S453) antibody in extracts from 293 cells.
Immunohistochemistry-Paraffin: MMP-3 Antibody - BSA Free [NB100-91878]

Immunohistochemistry-Paraffin: MMP-3 Antibody - BSA Free [NB100-91878]

Immunohistochemistry-Paraffin: MMP-3 Antibody [NB100-91878] - Analysis of mouse brain.
Immunohistochemistry-Paraffin: MMP-3 Antibody - BSA Free [NB100-91878]

Immunohistochemistry-Paraffin: MMP-3 Antibody - BSA Free [NB100-91878]

Immunohistochemistry-Paraffin: MMP-3 Antibody [NB100-91878] - Analyzes of paraffin-embedded human breast carcinoma tissue.

Applications for MMP-3 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:100

Western Blot

1:500- 1:1000
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reviewed Applications

Read 1 review rated 4 using NB100-91878 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MMP-3

Matrix metalloproteinase 3 (MMP3), also known as Stromelysin-1, SL-1, and STMY1, is a member of the matrix metalloproteinase (MMP) family. Members of the MMP family are involved in the breakdown of the extracellular matrix in response to normal physiological and disease processes; these processes include embryonic development, reproduction, tissue remodeling, arthritis and metastasis. The majority of MMP proteins are secreted into the extracellular matrix as inactive proproteins; they are activated when cleaved by an extracellular proteinase. The MMP3 gene contains four hemopexin-like domains, and is part of a cluster of MMP genes that localize to chromosome 11q22.3. This gene encodes the MMP3 enzyme that degrades fibronectin, laminin, gelatins (type I, III, IV and V), collagens (type III, IV, X, and IX), and cartilage proteoglycans. Additionally, the MMP3 enzyme activates procollagenase. Areas of interest for MMP3 include wound repair, progression of atherosclerosis, and tumor initiation.

Long Name

Matrix Metalloproteinase 3

Alternate Names

MMP3, Stromelysin 1

Entrez Gene IDs

4314 (Human); 17392 (Mouse); 171045 (Rat)

Gene Symbol

MMP3

UniProt

P08254, P28862

Additional MMP-3 Products

Product Documents for MMP-3 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for MMP-3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for MMP-3 Antibody - BSA Free

Customer Reviews for MMP-3 Antibody - BSA Free (1)

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  • MMP-3 Antibody - BSA Free
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Human lung macrophages and human lung cancer
    Species: Human and Mouse
    Verified Customer | Posted 04/25/2024
    To assess the functional secretory signatures associated with a more aggressive tumor microenvironment, we investigated the expression profile of the MMP family within cultured cells.
    MMP-3 Antibody - BSA Free NB100-91878

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Protocols

View specific protocols for MMP-3 Antibody - BSA Free (NB100-91878):

MMP-3 Antibody:
Immunohistochemistry-Paraffin Embedded Sections

Antigen Unmasking:
Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes.

Staining:
1. Wash sections in deionized water three times for 5 minutes each.
2. Wash sections in wash buffer for 5 minutes.
3. Block each section with 100-400 ul blocking solution for 1 hour at room temperature.
4. Remove blocking solution and add 100-400 ul diluted primary antibody. Incubate overnight at 4 C.
5. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
6. Add 100-400 ul biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
7. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
8. Add 100-400 ul Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
9. Wash sections three times in wash buffer for 5 minutes each.
10. Add 100-400 ul DAB substrate to each section and monitor staining closely.
11. As soon as the sections develop, immerse slides in deionized water.
12. Counterstain sections in hematoxylin.
13. Wash sections in deionized water two times for 5 minutes each.
14. Dehydrate sections.
15. Mount coverslips.

MMP-3 Antibody:
Western Blot Protocol

1. Perform SDS-PAGE on samples to be analyzed, loading 40 ug of total protein per lane.
2. Transfer proteins to membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
3. Stain according to standard Ponceau S procedure (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
4. Rinse the blot.
5. Block the membrane using standard blocking buffer for at least 1 hour.
6. Wash the membrane in wash buffer three times for 10 minutes each.
7. Dilute rabbit anti-MMP3 primary antibody in blocking buffer and incubate 1 hour at room temperature.
8. Wash the membrane in wash buffer three times for 10 minutes each.
9. Apply the diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
10. Wash the blot in wash buffer three times for 10 minutes each (this step can be repeated as required to reduce background).
11. Apply the detection reagent of choice in accordance with the manufacturers instructions.
Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2%.

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