MMP-7 Antibody (8T4R8)

Novus Biologicals | Catalog # NBP3-16067

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 8T4R8 expressed in HEK293
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Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-267 of human MMP7 (P09237). MRLTVLCAVCLLPGSLALPLPQEAGGMSELQWEQAQDYLKRFYLYDSETKNANSLEAKLKEMQKFFGLPITGMLNSRVIEIMQKPRCGVPDVAEYSLFPNSPKWTSKVVTYRIVSYTRDLPHITVDRLVSKALNMWGKEIPLHFRKVVWGTADIMIGFARGAHGDSYPFDGPGNTLAHAFAPGTGLGGDAHFDEDERWTDGSSLGINFLYAATHELGHSLGMGHSSDPNAVMYPTYGNGDPQNFKLSQDDIKGIQKLYGKRSNSRKK

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MMP-7 Antibody (8T4R8)

Western Blot: MMP-7 Antibody (8T4R8) [NBP3-16067]

Western Blot: MMP-7 Antibody (8T4R8) [NBP3-16067]

Western Blot: MMP-7 Antibody (9D8S10) [NBP3-16067] - Analysis of extracts of various cell lines, using MMP-7 antibody (NBP3-16067 ) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
MMP-7 Antibody (8T4R8)

Immunohistochemistry: MMP-7 Antibody (8T4R8) [NBP3-16067] -

Immunohistochemistry: MMP-7 Antibody (8T4R8) [NBP3-16067] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using MMP-7 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Applications for MMP-7 Antibody (8T4R8)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Reviewed Applications

Read 1 review rated 4 using NBP3-16067 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MMP-7

MMP7 is a Metallopeptidase M10A type protease. MMP-7 (Matrilysin) is constitutively produced by exocrine epithelial cells through out the body. It is capable of degrading casein, fibronectin, gelatins of types 1, III, IV, and V, in addition to activating procollagenase. It has been shown to play a key role in invasion by carcinomas.

Long Name

Matrix Metalloproteinase 7

Alternate Names

Matrilysin, MMP7

Gene Symbol

MMP7

Additional MMP-7 Products

Product Documents for MMP-7 Antibody (8T4R8)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for MMP-7 Antibody (8T4R8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for MMP-7 Antibody (8T4R8) (1)

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  • MMP-7 Antibody (8T4R8)
    Name: Shinford Sun
    Application: Western Blot
    Sample Tested: Cancer Cell lysate , human macrophage whole cell lysate and fibroblast cells
    Species: Human
    Verified Customer | Posted 05/24/2024
    MMP-7 is a small molecule secreted protein in the MMP family. We have attempted to detect it in various cancer cell lines and stromal cells. It is strongly expressed in tumor-associated fibroblasts and macrophages.
    MMP-7 Antibody (8T4R8) NBP3-16067

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Protocols

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