MNK1 Antibody (2C5) - Azide and BSA Free

Novus Biologicals | Catalog # H00008569-M07

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2C5

Format

Azide and BSA Free
View all MNK1 Primary Antibodies »

Product Specifications

Immunogen

MKNK1 (AAH02755.1, 1 a.a. ~ 465 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MVSSQKLEKPIEMGSSEPLPIADGDRRRKKKRRGRATDSLPGKFEDMYKLTSELLGEGAYAKVQGAVSLQNGKEYAVKIIEKQAGHSRSRVFREVETLYQCQRNKNILELIEFFEDDTRFYLVFEKLQGGSILAHIQKQKHFNEREASRVVRGVAAALDFLHTKDKVSLCHLGWSAMAPSGLTAAPTSLGSSDPPTSASQVAGTTGIAHRDLKPENILCESPEKVSPVKICDFDLGSGMKLNNSCTPITTPELTTPCGSAEYMAPEVVEVFTDQATFYDKRCDLWSLGVVLYIMLSGYPPFVGHCGADCGWDRGEVCRVCQNKLFESIQEGKYEFPDKDWAHISSEAKDLISKLLVRDAKQRLSAAQVLQHPWVQGQAPEKGLPTPQVLQRNSSTMDLTLFAAEAIALNRLLSQHEENELAEEPEALADGLCSMKLSPPCKSRLARRRALAQAGRGEDRSPPTAL

Localization

Isoforms 1 and 2 cytoplasmic, isoform 3 nuclear

Specificity

MKNK1 - MAP kinase interacting serine/threonine kinase 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for MNK1 Antibody (2C5) - Azide and BSA Free

Western Blot: MNK1 Antibody (2C5) [H00008569-M07]

Western Blot: MNK1 Antibody (2C5) [H00008569-M07]

Western Blot: MNK1 Antibody (2C5) [H00008569-M07] - Analysis of MKNK1 expression in transfected 293T cell line by MKNK1 monoclonal antibody (M07), clone 2C5. Lane 1: MKNK1 transfected lysatE (51.3 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: MNK1 Antibody (2C5) [H00008569-M07]

Immunocytochemistry/ Immunofluorescence: MNK1 Antibody (2C5) [H00008569-M07]

Immunocytochemistry/Immunofluorescence: MNK1 Antibody (2C5) [H00008569-M07] - Analysis of monoclonal antibody to MKNK1 on HeLa cell. Antibody concentration 10 ug/ml
ELISA: MNK1 Antibody (2C5) [H00008569-M07]

ELISA: MNK1 Antibody (2C5) [H00008569-M07]

ELISA: MNK1 Antibody (2C5) [H00008569-M07] - Detection limit for recombinant GST tagged MKNK1 is approximately 3ng/ml as a capture antibody.

Applications for MNK1 Antibody (2C5) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MNK1

MNK1 may play a role in the response to environmental stress and cytokines. It appears to regulate transcription by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap.

Long Name

MAP kinase-interacting serine/threonine-protein kinase 1

Alternate Names

MKNK1

Entrez Gene IDs

8569 (Human)

Gene Symbol

MKNK1

OMIM

606724 (Human)

UniProt

AAH02755 (Human)

Additional MNK1 Products

Product Documents for MNK1 Antibody (2C5) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for MNK1 Antibody (2C5) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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