Monoamine Oxidase B Antibody

Novus Biologicals | Catalog # NB100-2826

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Monoamine Oxidase B Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-HKARKLARLTKEE corresponding to internal region according to NP_000889.3.

Specificity

This antibody is not expected to cross-react with MAOA.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Monoamine Oxidase B Antibody

Western Blot: Monoamine Oxidase B Antibody [NB100-2826]

Western Blot: Monoamine Oxidase B Antibody [NB100-2826]

Western Blot: Monoamine Oxidase B Antibody [NB100-2826] - Staining of Mouse Liver lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: Monoamine Oxidase B Antibody [NB100-2826]

Immunocytochemistry/ Immunofluorescence: Monoamine Oxidase B Antibody [NB100-2826]

Immunocytochemistry/Immunofluorescence: Monoamine Oxidase B Antibody [NB100-2826] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Flow Cytometry: Monoamine Oxidase B Antibody [NB100-2826]

Flow Cytometry: Monoamine Oxidase B Antibody [NB100-2826]

Flow Cytometry: Monoamine Oxidase B Antibody [NB100-2826] - Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Western Blot: Monoamine Oxidase B Antibody [NB100-2826]

Western Blot: Monoamine Oxidase B Antibody [NB100-2826]

Western Blot: Monoamine Oxidase B Antibody [NB100-2826] - Staining of Human Liver (A) with antibody at 0.1 ug/mL and Testes (B) and Breast Cancer (C) lysate with antibody at 0.3ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.

Applications for Monoamine Oxidase B Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Peptide ELISA

Detection limit 1:32000

Western Blot

0.1 - 0.3 ug/mL
Application Notes
Western blot: Approx 60 kDa band observed in Human and Mouse Liver lysates and approx. 55-60 kDa in Human Testis and Breast cancer lysates (calculated MW of 58.8 kDa according to NP_000889.3).

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Monoamine Oxidase B

The protein encoded by this gene belongs to the flavin monoamine oxidase family. It is a enzyme located in the mitochondrial outer membrane. It catalyzes the oxidative deamination of biogenic and xenobiotic amines and plays an important role in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. This protein preferentially degrades benzylamine and phenylethylamine.

Long Name

Amine oxidase [flavin-containing] B

Alternate Names

EC 1.4.3.21, EC 1.4.3.4, MAO-B, MAOB

Entrez Gene IDs

4129 (Human); 109731 (Mouse)

Gene Symbol

MAOB

UniProt

NP_000889.3 (Human)

Additional Monoamine Oxidase B Products

Product Documents for Monoamine Oxidase B Antibody

Certificate of Analysis

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Product Specific Notices for Monoamine Oxidase B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Monoamine Oxidase B Antibody

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Protocols

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