Monoglyceride Lipase Antibody

Novus Biologicals | Catalog # NB100-96920

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Monoglyceride Lipase Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-QDLPHLVNADGQY corresponding to internal region (near N-Terminus) according to NP_001003794.1.

Epitope

QDLPHLVNADGQY

Specificity

This antibody is expected to recognize both reported isoforms (NP_009214.1; NP_001003794.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Monoglyceride Lipase Antibody

Western Blot: Monoglyceride Lipase Antibody [NB100-96920]

Western Blot: Monoglyceride Lipase Antibody [NB100-96920]

Western Blot: Monoglyceride Lipase Antibody [NB100-96920] - Staining of Mouse Adipose lysate (35 ug protein in RIPA buffer). Antibody at 0.5 ug/mL. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: Monoglyceride Lipase Antibody [NB100-96920]

Immunocytochemistry/ Immunofluorescence: Monoglyceride Lipase Antibody [NB100-96920]

Immunocytochemistry/Immunofluorescence: Monoglyceride Lipase Antibody [NB100-96920] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic and endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Immunohistochemistry-Paraffin: Monoglyceride Lipase Antibody [NB100-96920]

Immunohistochemistry-Paraffin: Monoglyceride Lipase Antibody [NB100-96920]

Immunohistochemistry-Paraffin: Monoglyceride Lipase Antibody [NB100-96920] - Negative Control showing staining of paraffin embedded Human Liver, with no primary antibody.
Flow Cytometry: Monoglyceride Lipase Antibody [NB100-96920]

Flow Cytometry: Monoglyceride Lipase Antibody [NB100-96920]

Flow Cytometry: Monoglyceride Lipase Antibody [NB100-96920] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Immunocytochemistry/ Immunofluorescence: Monoglyceride Lipase Antibody [NB100-96920]

Immunocytochemistry/ Immunofluorescence: Monoglyceride Lipase Antibody [NB100-96920]

Immunocytochemistry/Immunofluorescence: Monoglyceride Lipase Antibody [NB100-96920] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear and cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Immunohistochemistry-Paraffin: Monoglyceride Lipase Antibody [NB100-96920]

Immunohistochemistry-Paraffin: Monoglyceride Lipase Antibody [NB100-96920]

Immunohistochemistry-Paraffin: Monoglyceride Lipase Antibody [NB100-96920] - Staining of paraffin embedded Human Liver. Antibody at 5 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Applications for Monoglyceride Lipase Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Immunohistochemistry

5 ug/mL

Immunohistochemistry-Paraffin

5 ug/mL

Peptide ELISA

Detection limit 1:128000

Western Blot

0.3 - 1 ug/mL
Application Notes
Western blot: Approx 35 kDa band observed in Mouse Adipose lysates and in preliminary testing of Human, Cerebellum lysate and in lysate of cell line NIH3T3 (calculated MW of 33.3 kDa according to NP_001003794.1).

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Monoglyceride Lipase

Monoglyceride lipase (MGLL; EC 3.1.1.23) functions together with hormone-sensitive lipase (LIPE; MIM 151750) to hydrolyze intracellular triglyceride stores in adipocytes and other cells to fatty acids and glycerol. MGLL may also complement lipoprotein lipase (LPL; MIM 238600) in completing hydrolysis of monoglycerides resulting from degradation of lipoprotein triglycerides (Karlsson et al., 2001 [PubMed 11470505]).[supplied by OMIM]

Alternate Names

HUK5, MAGL, MGLL

Entrez Gene IDs

11343 (Human); 23945 (Mouse); 29254 (Rat)

Gene Symbol

MGLL

UniProt

Q99685

Additional Monoglyceride Lipase Products

Product Documents for Monoglyceride Lipase Antibody

Certificate of Analysis

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Product Specific Notices for Monoglyceride Lipase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Monoglyceride Lipase Antibody

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Protocols

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