Monomethyl Arginine Antibody (5D1) - Azide and BSA Free

Novus Biologicals | Catalog # NB500-122

Novus Biologicals
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Key Product Details

Species Reactivity

All Species

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 5D1

Format

Azide and BSA Free
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Product Specifications

Immunogen

Modified monomethyl arginine

Specificity

This reacts with free and bound NG-monomethyl arginine. There is no cross reaction with either NG-NG-dimethyl arginine or arginine.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Applications for Monomethyl Arginine Antibody (5D1) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:100 - 1:10 000

Immunohistochemistry-Paraffin

1:100-1:10000

Western Blot

1:500-1:5000

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Lyophilized powder, reconstituted in distilled water.

Formulation

0.1M Tris, 0.1M Glycine and 2% Sucrose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Monomethyl Arginine

This antibody will be of central importance in analysing the methylation status of chromatin and transcription factors. Since it recognizes monomethyl arginine only it can be used in parallel with ab413 (detects dimethyl arginine only) and ab412 (detects both monomethyl and dimethyl arginine) to monitor the exact modification status.

Alternate Names

Arginine (mono methyl), Di methyl Arginine, Dimethyl Arginine, Methyl Arginine, Mono methyl Arginine, Monomethyl Arginine

Additional Monomethyl Arginine Products

Product Documents for Monomethyl Arginine Antibody (5D1) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Monomethyl Arginine Antibody (5D1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Monomethyl Arginine Antibody (5D1) - Azide and BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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