Mouse ADAM10 Ectodomain Antibody

Catalog # Availability Size / Price Qty
Product Details
Citations (10)
Supplemental Products
Reviews (1)

Mouse ADAM10 Ectodomain Antibody Summary

Species Reactivity
Detects mouse ADAM10 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human (rh) rhADAM10 is observed and no cross-reactivity with rhADAM8, recombinant mouse (rm) ADAM9, rmADAM15, rhBACE, or rhTACE is observed.
Monoclonal Rat IgG2A Clone # 139712
Protein A or G purified from hybridoma culture supernatant
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse ADAM10
Accession # O35598
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
Recombinant Mouse ADAM10 (Catalog # 946-AD) under non-reducing conditions only
Flow Cytometry
2.5 µg/106 cells
Mouse splenocytes
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ADAM10

ADAM10 (also known as Kuzbanian, mammalian disintegrin metalloprotease, myelin-associated metalloproteinase) is a member of the ADAM family that contains a disintegrin and metalloprotease-like domain (1, 2). Like other membrane-anchored ADAMs, ADAM10 consists of the following domains, pro with a cysteine switch and furin cleavage sequence, catalytic with the zinc-binding site and Met-turn expected for reprolysins, disintegrin-like, cysteine-rich, EGF-like, transmembrane, and cytoplasmic. ADAM10 is highly conserved, with 97% amino acid identity between mouse, rat, bovine and human and 45% identity between mouse and Drosophila. The active enzyme processes notch, notch ligand delta, and amyloid protein precursor at the alpha site, playing an important role in neurogenesis (3, 4). It also processes the 26 kDa membrane-anchored pro-tumor necrosis factor-alpha (TNF-alpha ) to the 17 kDa mature TNF-alpha (5). It cleaves myelin basic protein and type IV collagen (6, 7). ADAM10 is widely expressed in tissues and resides both on the cell surface and in the cell (8, 9).

  1. Rooke, J. et al. (1996) Science 273:1227.
  2. Pan, D. and Rubin, G.M. (1997) Cell 90:271.
  3. Qi, H. et al. (1999) Science 283:91.
  4. Lammich, S. et al. (1999) Proc. Natl. Acad. Sci. USA 96:3922.
  5. Rosendahl, M.S. et al. (1997) J. Biol. Chem. 272:24588.
  6. Chantry, A. et al. (1989) J. Biol. Chem. 264:21603.
  7. Millichip, M.I. et al. (1998) Biochem. Biophys. Res. Comm. 245:594.
  8. Chantry, A. and Glynn, P. (1990) Biochem. J. 268:245.
  9. Fahrenholz, F.S. et al. (2000) Ann. N.Y. Acad. Sci. 920:215.
Long Name
A Disintegrin and Metalloprotease-like Domain 10
Entrez Gene IDs
102 (Human); 11487 (Mouse)
Alternate Names
a disintegrin and metalloprotease domain 10; a disintegrin and metalloproteinase domain 10; AD10; ADAM 10; ADAM metallopeptidase domain 10; ADAM10; CD156c antigen; CD156c; CDw156; disintegrin and metalloproteinase domain-containing protein 10; EC 3.4.24; HsT18717; kuz; Kuzbanian protein homolog; Kuzbanian; MADM; MADMEC; Mammalian disintegrin-metalloprotease

Product Datasheets

You must select a language.


Citations for Mouse ADAM10 Ectodomain Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. ADAM10 facilitates rapid neural stem cell cycling and proper positioning within the subventricular zone niche via JAMC/RAP1Gap signaling
    Authors: N McMillan, GW Kirschen, S Desai, E Xia, SE Tsirka, A Aguirre
    Neural regeneration research, 2022;17(11):2472-2483.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. CXCR6+CD4+ T cells promote mortality during Trypanosoma brucei infection
    Authors: G Liu, O Abas, AB Strickland, Y Chen, M Shi
    PloS Pathogens, 2021;17(10):e1009968.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Macroautophagy in Dendritic Cells Controls the Homeostasis and Stability of Regulatory T Cells
    Authors: J Niven, N Madelon, N Page, A Caruso, G Harlé, S Lemeille, CA Seemayer, S Hugues, M Gannagé
    Cell Rep, 2019;28(1):21-29.e6.
    Species: Transgenic Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Tetraspanin 3: A central endocytic membrane component regulating the expression of ADAM10, presenilin and the amyloid precursor protein
    Authors: Paul Saftig
    Biochim. Biophys. Acta, 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. The sheddase ADAM10 is a potent modulator of prion disease.
    Authors: Altmeppen, Hermann, Prox, Johannes, Krasemann, Susanne, Puig, Berta, Kruszewski, Katharin, Dohler, Frank, Bernreuther, Christia, Hoxha, Ana, Linsenmeier, Luise, Sikorska, Beata, Liberski, Pawel P, Bartsch, Udo, Saftig, Paul, Glatzel, Markus
    Elife, 2015;4(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  6. Pyroptosis of resident macrophages differentially orchestrates inflammatory responses to Staphylococcus aureus in resistant and susceptible mice.
    Authors: Accarias S, Lugo-Villarino G, Foucras G, Neyrolles O, Boullier S, Tabouret G
    Eur J Immunol, 2015;45(3):794-806.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Extracellular vesicle-mediated transfer of genetic information between the hematopoietic system and the brain in response to inflammation.
    Authors: Ridder K, Keller S, Dams M, Rupp A, Schlaudraff J, Turco D, Starmann J, Macas J, Karpova D, Devraj K, Depboylu C, Landfried B, Arnold B, Plate K, Hoglinger G, Sultmann H, Altevogt P, Momma S
    PLoS Biol, 2014;12(6):e1001874.
    Species: Mouse
    Sample Types: Extracellular Vesicles
    Applications: Western Blot
  8. Adrenergic regulation of IgE involves modulation of CD23 and ADAM10 expression on exosomes.
    Authors: Padro C, Shawler T, Gormley M, Sanders V
    J Immunol, 2013;191(11):5383-97.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Deletion of Adam10 in endothelial cells leads to defects in organ-specific vascular structures.
    Authors: Glomski K, Monette S, Manova K, de Strooper B, Saftig P, Blobel CP
    Blood, 2011;118(4):1163-74.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  10. A disintegrin and metalloproteinase 10-mediated cleavage and shedding regulates the cell surface expression of CXC chemokine ligand 16.
    Authors: Gough PJ, Garton KJ, Wille PT, Rychlewski M, Dempsey PJ, Raines EW
    J. Immunol., 2004;172(6):3678-85.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization


No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Mouse ADAM10 Ectodomain Antibody

Average Rating: 4 (Based on 1 Review)

5 Star
4 Star
3 Star
2 Star
1 Star

Have you used Mouse ADAM10 Ectodomain Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:

Mouse ADAM10 Ectodomain Antibody
By Anonymous on 10/26/2015
Application: WB Sample Tested: 4T1.2 mouse mammary carcinoma cells Species: Mouse

Buffer: TBST
Dilution: 1/200