Mouse alpha 1-Acid Glycoprotein Antibody Summary
Accession # Q60590
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Mouse alpha 1-Acid Glycoprotein by Western Blot. Western blot shows lysates of mouse liver tissue. PVDF Membrane was probed with 2 µg/mL of Rat Anti-Mouse a1-Acid Glycoprotein Monoclonal Antibody (Catalog # MAB5934) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for a1-Acid Glycoprotein at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: alpha 1-Acid Glycoprotein
alpha 1-Acid Glycoprotein (AGP; also OMD/Orosomucoid) is a 40‑46 kDa member of the immunocalin subfamily, lipocalin family of molecules. In mouse, circulating AGP is principally the product of hepatocytes that originates from multiple related genes (AGP-1, -2 & -3 in Mus musculus). Circulating AGP-1 and -2 are both 189 amino acids (aa) in length, the principal sources of protein, and show 83% aa identity; AGP-3 contributes little to the AGP pool. In mouse blood, AGP is normally 200‑400 μg/mL. In response to inflammatory mediators (IL-6; IL-1), its concentration will rise 2 to 10 fold. More importantly, a complex glycosylation pattern will also change, transitioning from modestly branched to highly branched oligosaccharides. This change is reflected in its bioactivity, which has been shown to be a function of carbohydrate branching. AGP is generally considered to be a suppressor of inflammation. Rat and human AGP share only 70% and 47% aa identity with mouse AGP, respectively.
Citation for Mouse alpha 1-Acid Glycoprotein Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Gene expression profiles among murine strains segregate with distinct differences in the progression of radiation-induced lung disease
Authors: IL Jackson, F Baye, CP Goswami, BP Katz, A Zodda, R Pavlovic, G Gurung, D Winans, Z Vujaskovic
Dis Model Mech, 2017;0(0):.
Sample Types: Tissue Homogenates
Applications: Western Blot
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