Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # 628110
View all alpha 1-Acid Glycoprotein Primary Antibodies »

Product Specifications

Immunogen

Mouse serum-derived alpha 1‑Acid Glycoprotein
Accession # Q60590

Specificity

Detects mouse alpha 1‑Acid Glycoprotein in Western blots.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Scientific Data Images for Mouse alpha 1‑Acid Glycoprotein Antibody

Detection of Mouse a1-Acid Glycoprotein antibody by Western Blot.

Detection of Mouse alpha 1-Acid Glycoprotein by Western Blot.

Western blot shows lysates of mouse liver tissue. PVDF Membrane was probed with 2 µg/mL of Rat Anti-Mouse a1-Acid Glycoprotein Monoclonal Antibody (Catalog # MAB5934) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for a1-Acid Glycoprotein at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group.

Applications for Mouse alpha 1‑Acid Glycoprotein Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Mouse plasma, see our available Western blot detection antibodies

Western Blot

2 µg/mL
Sample: Mouse liver tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: alpha 1-Acid Glycoprotein

alpha 1-Acid Glycoprotein (AGP; also OMD/Orosomucoid) is a 40‑46 kDa member of the immunocalin subfamily, lipocalin family of molecules. In mouse, circulating AGP is principally the product of hepatocytes that originates from multiple related genes (AGP-1, -2 & -3 in Mus musculus). Circulating AGP-1 and -2 are both 189 amino acids (aa) in length, the principal sources of protein, and show 83% aa identity; AGP-3 contributes little to the AGP pool. In mouse blood, AGP is normally 200‑400 μg/mL. In response to inflammatory mediators (IL-6; IL-1), its concentration will rise 2 to 10 fold. More importantly, a complex glycosylation pattern will also change, transitioning from modestly branched to highly branched oligosaccharides. This change is reflected in its bioactivity, which has been shown to be a function of carbohydrate branching. AGP is generally considered to be a suppressor of inflammation. Rat and human AGP share only 70% and 47% aa identity with mouse AGP, respectively.

Alternate Names

AGP, AGP1, AGPA, alpha 1Acid Glycoprotein, ORM1, Orosomucoid

Entrez Gene IDs

5004 (Human); 18405 (Mouse); 24614 (Rat)

Gene Symbol

ORM1

UniProt

Q60590

Additional alpha 1-Acid Glycoprotein Products

Product Documents for Mouse alpha 1‑Acid Glycoprotein Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse alpha 1‑Acid Glycoprotein Antibody

For research use only

Citations for Mouse alpha 1‑Acid Glycoprotein Antibody

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