Detection of Mouse Angiopoietin‑1 by Western Blot.
Western blot shows lysates of mouse heart tissue and mouse kidney tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse Angiopoietin‑1 Monoclonal Antibody (Catalog # MAB8220) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Angiopoietin‑1 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Angiopoietin-1 (Ang-1) and Angiopoietin-2 (Ang-2) are two closely related secreted ligands which bind with similar affinity to Tie-2, a receptor tyrosine kinase with immunoglobulin and epidermal growth factor homology domains expressed primarily on endothelial cells and early hematopoietic cells. Tie-2 and angiopoietins have been shown to play critical roles in embryogenic angiogenesis and in maintaining the integrity of the adult vasculature (1).
Ang-1 cDNA encodes a 498 amino acid (aa) residue precursor protein that contains a coiled-coiled domain near the amino-terminus and a fibrinogen-like domain at the C-terminus. Human Ang-1 shares approximately 97% and 60% amino acid sequence identity with mouse Ang-1 and human Ang-2, respectively (1, 2). Ang-1 activates Tie-2 signaling on endothelial cells to promote chemotaxis, cell survival, cell sprouting, vessel growth and stabilization (1, 3, 4). Ang-2 has alternatively been reported to be an antagonist for Ang-1 induced Tie-2 signaling as well as an agonist for Tie-2 signaling, depending on the cell context (5).
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Witzenbichler, B. et al. (1998) J. Biol. Chem. 273:18514.
Papapetropoulos, A. et al. (1999) Lab. Inest. 79:213.
Teichert-Kuliszewska, K. et al. (2001) Cardiovasc. Res. 49:659.
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