Mouse ASCL1/Mash1 Antibody

R&D Systems | Catalog # MAB2567

R&D Systems
Discontinued Product
MAB2567 has been discontinued. An alternative/replacement product is available: AF2567. View all ASCL1/Mash1 products.

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 290622
View all ASCL1/Mash1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse ASCL1/Mash1
His74-Asp173 (Gln160Glu)
Accession # Q53WV9

Specificity

Detects mouse ASCL1/Mash1 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Mouse ASCL1/Mash1 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed NTera-2 human testicular embryonic carcinoma cell line treated with retinoic acid

Western Blot

1 µg/mL
Sample: Recombinant Mouse ASCL1/Mash1

Reviewed Applications

Read 1 review rated 3 using MAB2567 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ASCL1/Mash1

ASCL1 (achaete-scute complex-like 1), also known as Mash1, is a member of the basic helix-loop-helix family of transcription factors. It is required for neuronal commitment and differentiation. Within the region used as an immunogen, mouse ASCL1 shares 97% amino acid sequence identity with human ASCL1.

Long Name

Achaete Scute Complex-like 1

Alternate Names

ASCL1, ASH1, HASH1, Mash1

Entrez Gene IDs

429 (Human); 17172 (Mouse)

Gene Symbol

ASCL1

UniProt

Q53WV9

Additional ASCL1/Mash1 Products

Product Documents for Mouse ASCL1/Mash1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse ASCL1/Mash1 Antibody

For research use only

Citations for Mouse ASCL1/Mash1 Antibody

Customer Reviews for Mouse ASCL1/Mash1 Antibody (1)

3 out of 5
1 Customer Rating
5 Stars
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3 Stars
100%
2 Stars
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1 Stars
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  • Mouse ASCL1/Mash1 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Embryonic brain
    Species: Mouse
    Verified Customer | Posted 08/10/2017

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Protocols

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FAQs

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