Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all ASCL1/Mash1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse ASCL1/Mash1
His74-Asp173 (Gln160Glu)
Accession # Q53WV9

Specificity

Detects mouse ASCL1/Mash1 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse ASCL1/Mash1 Antibody

ASCL1/Mash1 in Mouse Embryo.

ASCL1/Mash1 was detected in immersion fixed paraffin-embedded sections of mouse embryo (13 d.p.c., cross-section through developing CNS) using Goat Anti-Mouse ASCL1/Mash1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2567) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Mouse ASCL1/Mash1 Antibody

Application
Recommended Usage

Immunohistochemistry

3-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of mouse embryo (13 d.p.c., cross-section through developing CNS)

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse ASCL1/Mash1

Reviewed Applications

Read 3 reviews rated 4 using AF2567 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ASCL1/Mash1

Mash1, also known as achaete-scute complex-like 1 (ASCL1), is a member of the basic helix-loop-helix family of transcription factors. It is required for neuronal commitment and differentiation. The mouse immunogen used to generate this antibody shares 97% amino acid sequence identity with the equivalent region of human Mash1.

Long Name

Achaete Scute Complex-like 1

Alternate Names

ASCL1, ASH1, HASH1, Mash1

Entrez Gene IDs

429 (Human); 17172 (Mouse)

Gene Symbol

ASCL1

UniProt

Q53WV9

Additional ASCL1/Mash1 Products

Product Documents for Mouse ASCL1/Mash1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse ASCL1/Mash1 Antibody

For research use only

Citations for Mouse ASCL1/Mash1 Antibody

Customer Reviews for Mouse ASCL1/Mash1 Antibody (3)

4 out of 5
3 Customer Ratings
5 Stars
33%
4 Stars
33%
3 Stars
33%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Mouse ASCL1/Mash1 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Brain (cross-section through blood vessel)
    Species: Mouse
    Verified Customer | Posted 08/23/2024
    Mouse ASCL1/Mash1 Antibody AF2567
  • Mouse ASCL1/Mash1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: 293T human embryonic kidney cell line and Embryo tissue
    Species: Mouse
    Verified Customer | Posted 04/04/2022
    Mouse ASCL1/Mash1 Antibody AF2567
  • Mouse ASCL1/Mash1 Antibody
    Name: Michelle Richardson
    Application: Western Blot
    Sample Tested: 293T human embryonic kidney cell line
    Species: Human
    Verified Customer | Posted 04/10/2017
    25ug cell lysate run under reduced conditions.
    Mouse ASCL1/Mash1 Antibody AF2567

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Protocols

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