Detection of BCMA/TNFRSF17 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse BCMA/TNFRSF17 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB593F) and Rat Anti-Mouse B220/CD45R APC‑conjugated Monoclonal Antibody (Catalog # FAB1217A). Quadrant markers were set based on control antibody staining. View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
BCMA (B Cell Maturation Antigen),
also known as TNFRSF17, is a 22-26 kDa member of the TNFR superfamily. It is a type III transmembrane (TM) protein one that is synthesized as a type I TM protein that has a restricted expression pattern.
Among the cells recognized to express BCMA are bone marrow plasma cells
(PCs), adipocytes and stratum basale keratinocytes. At least some human BCMA is now known to be
N-glycosylated, a modification that affects both its affinity for ligand, and
its half-life on the cell surface.
Mouse, by contrast, has no potential N-linked site, and any
glycosylation would have to be either O-linked, or N-linked through the
generation of an alternative splice form.
In any case, and although the predicted MW of mouse BCMA is 20 kDa, it
appears to run anomalously at 22-26 kDa in SDS-PAGE. Mouse BCMA is 185 amino acids (aa) in
length. It contains a long, 115 aa
cytoplasmic region and a short 49 aa extracellular domain characterized by two
cysteine-rich segments. Ligands for BCMA
are trimers, and include APRIL and BAFF homotrimers, plus APRIL:BAFF
heterotrimers. In human, BCMA is known
to be proteolytically cleaved and released, acting as an antagonist to APRIL
AND BAFF signaling; in mouse, it is unclear if cleavage occurs. BCMA activation on PCs promotes PC survival,
while on adipocytes, BCMA signaling downregulates adiponectin and leptin
production, and on keratinocytes, BCMA ligation induces IL-6 and GM-CSF
secretion. Over aa 1-49, mouse BCMA shares
65% and 90% aa sequence identity with human and rat BCMA, respectively.
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