Mouse BCMA/TNFRSF17 Fluorescein-conjugated Antibody

(5 citations)   
  • Species Reactivity
  • Specificity
    Detects mouse BCMA in direct ELISAs. In direct ELISAs, this antibody does not cross-react with recombinant human (rh) BCMA or rhTACI.
  • Source
    Monoclonal Rat IgG1 Clone # 161616
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse BCMA
    Accession # O88472
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of BCMA/TNFRSF17 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse BCMA/TNFRSF17 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB593F) and Rat Anti-Mouse B220/CD45R APC‑conjugated Monoclonal Antibody (Catalog # FAB1217A). Quadrant markers were set based on control antibody staining. View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: BCMA/TNFRSF17
BCMA (B Cell Maturation Antigen), also known as TNFRSF17, is a 22-26 kDa member of the TNFR superfamily. It is a type III transmembrane (TM) protein one that is synthesized as a type I TM protein that has a restricted expression pattern. Among the cells recognized to express BCMA are bone marrow plasma cells (PCs), adipocytes and stratum basale keratinocytes. At least some human BCMA is now known to be N-glycosylated, a modification that affects both its affinity for ligand, and its half-life on the cell surface. Mouse, by contrast, has no potential N-linked site, and any glycosylation would have to be either O-linked, or N-linked through the generation of an alternative splice form. In any case, and although the predicted MW of mouse BCMA is 20 kDa, it appears to run anomalously at 22-26 kDa in SDS-PAGE. Mouse BCMA is 185 amino acids (aa) in length. It contains a long, 115 aa cytoplasmic region and a short 49 aa extracellular domain characterized by two cysteine-rich segments. Ligands for BCMA are trimers, and include APRIL and BAFF homotrimers, plus APRIL:BAFF heterotrimers. In human, BCMA is known to be proteolytically cleaved and released, acting as an antagonist to APRIL AND BAFF signaling; in mouse, it is unclear if cleavage occurs. BCMA activation on PCs promotes PC survival, while on adipocytes, BCMA signaling downregulates adiponectin and leptin production, and on keratinocytes, BCMA ligation induces IL-6 and GM-CSF secretion. Over aa 1-49, mouse BCMA shares 65% and 90% aa sequence identity with human and rat BCMA, respectively.
  • Long Name:
    B Cell Maturation Factor
  • Entrez Gene IDs:
    608 (Human); 21935 (Mouse); 287034 (Rat)
  • Alternate Names:
    B cell maturation antigen; B-cell maturation protein; BCMAtumor necrosis factor receptor superfamily member 17; BCMB-cell maturation factor; CD269 antigen; CD269; TNFRSF17; tumor necrosis factor receptor superfamily, member 17
Related Research Areas

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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Sample Type
  1. PD-L1hi B cells are critical regulators of humoral immunity.
    Authors: Khan A, Hams E, Floudas A, Sparwasser T, Weaver C, Fallon P
    Nat Commun, 2015;6(0):5997.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  2. Impaired B cell receptor signaling is responsible for reduced TACI expression and function in X-linked immunodeficient mice.
    Authors: Uslu K, Coleman A, Allman W, Katsenelson N, Bram R, Alugupalli K, Akkoyunlu M
    J Immunol, 2014;192(8):3582-95.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  3. Secreted IgM enhances B cell receptor signaling and promotes splenic but impairs peritoneal B cell survival.
    Authors: Notley CA, Baker N, Ehrenstein MR
    J. Immunol., 2010;184(7):3386-93.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  4. FcgammaRIIB signals inhibit BLyS signaling and BCR-mediated BLyS receptor up-regulation.
    Authors: Crowley JE, Stadanlick JE, Cambier JC, Cancro MP
    Blood, 2009;113(7):1464-73.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  5. Deficient TACI expression on B lymphocytes of newborn mice leads to defective Ig secretion in response to BAFF or APRIL.
    Authors: Kanswal S, Katsenelson N, Selvapandiyan A, Bram RJ, Akkoyunlu M
    J. Immunol., 2008;181(2):976-90.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG1 Fluorescein‑conjugated Control Antibody

Ctrl IC005F 2
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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