Mouse beta 2-Microglobulin Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse  beta 2‑Microglobulin in direct ELISAs and Western blots.
  • Source
    Monoclonal Rat IgG2A Clone # 893803
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse beta 2‑Microglobulin
    Met1-Met119
    Accession # P01887
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse beta 2‑Microglobulin by Western Blot. Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line, J774A.1 mouse reticulum cell sarcoma macrophage cell line, and Hepa 1‑6 mouse hepatoma cell line. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse beta 2‑Microglobulin Monoclonal Antibody (Catalog # MAB8325) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for beta 2‑Microglobulin at approximately 13 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: beta 2-Microglobulin
Beta 2-Microglobulin (b2M) is a 12 kDa secreted polypeptide that serves as the light chain of Class I MHC molecules. Possessing an Ig-like domain, b2M noncovalently associates with both 44 kDa classical (HLA-A, -B, -C) and 40 kDa non-classical (HLA-E, -F, -G) Class I MHC heavy chains as well as with 43-49 kDa Class I non-MHC heavy chains (CD1). b2M is expressed on nearly all nucleated cells, with neurons being a notable exception. Circulating b2M is generated during normal HLA turnover. It can also dissociate from the MHC complex and circulate as full length and N-terminal truncated peptides of 93, 91, and 90 amino acids. It has been measured in a variety of body fluids, including serum, plasma, saliva, CSF, and urine. b2M freely passes through the glomerular membrane, but it is 99% actively reabsorbed and degraded in the proximal tubule cells. Circulating b2M levels are elevated in rheumatoid arthritis, systemic lupus erythematosus, viral infections, and conditions with decreased glomerular filtration. Human b2M shares 70% and 75% amino acid sequence identity with mouse and rat b2M, respectively.
  • Entrez Gene IDs:
    567 (Human); 12010 (Mouse); 24223 (Rat)
  • Alternate Names:
    B2M; beta 2Microglobulin; beta 2-Microglobulin; beta chain of MHC class I molecules; beta-2-microglobin; beta-2-microglobulin
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A Isotype Control

Ctrl MAB006 90  
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Normal Rat IgG Control

Ctrl 6-001-A 9
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Normal Rat IgG Control (Azide Free)

Ctrl 6-001-F 1
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Rat IgG2A Isotype Control

Ctrl MAB006R  
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Secondary Antibodies
Description Application Cat# Citations Images  

Rat IgG HRP-conjugated Antibody

WB, Simple Western HAF005 7  
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Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Flow F0113 2  
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Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL013 2
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Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0105B 2  
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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015 1
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Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
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Goat Anti-Rat IgG Antibody

WB AF005 1
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
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Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
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