Mouse beta 2-Microglobulin Antibody

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Detection of Mouse  beta 2‑Microglobulin by Western Blot.
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Product Details
Citations (1)
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Mouse beta 2-Microglobulin Antibody Summary

Species Reactivity
Detects mouse  beta 2‑Microglobulin in direct ELISAs and Western blots.
Monoclonal Rat IgG2A Clone # 893803
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant mouse beta 2‑Microglobulin
Accession # P01887
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse  beta <SUB>2</SUB>-Microglobulin antibody by Western Blot. View Larger

Detection of Mouse beta 2‑Microglobulin by Western Blot. Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line, J774A.1 mouse reticulum cell sarcoma macrophage cell line, and Hepa 1-6 mouse hepatoma cell line. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse beta 2-Microglobulin Monoclonal Antibody (Catalog # MAB8325) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for beta 2-Microglobulin at approximately 13 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: beta 2-Microglobulin

Beta 2-Microglobulin (b2M) is a 12 kDa secreted polypeptide that serves as the light chain of Class I MHC molecules. Possessing an Ig-like domain, b2M noncovalently associates with both 44 kDa classical (HLA-A, -B, -C) and 40 kDa non-classical (HLA-E, -F, -G) Class I MHC heavy chains as well as with 43-49 kDa Class I non-MHC heavy chains (CD1). b2M is expressed on nearly all nucleated cells, with neurons being a notable exception. Circulating b2M is generated during normal HLA turnover. It can also dissociate from the MHC complex and circulate as full length and N-terminal truncated peptides of 93, 91, and 90 amino acids. It has been measured in a variety of body fluids, including serum, plasma, saliva, CSF, and urine. b2M freely passes through the glomerular membrane, but it is 99% actively reabsorbed and degraded in the proximal tubule cells. Circulating b2M levels are elevated in rheumatoid arthritis, systemic lupus erythematosus, viral infections, and conditions with decreased glomerular filtration. Human b2M shares 70% and 75% amino acid sequence identity with mouse and rat b2M, respectively.

Entrez Gene IDs
567 (Human); 12010 (Mouse); 24223 (Rat); 101867173 (Cynomolgus Monkey)
Alternate Names
B2M; beta 2Microglobulin; beta 2-Microglobulin; beta chain of MHC class I molecules; beta-2-microglobin; beta-2-microglobulin

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Citation for Mouse beta 2-Microglobulin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Inhibition of MICA and MICB Shedding Elicits NK cell-mediated Immunity against Tumors Resistant to Cytotoxic T cells
    Authors: L Ferrari de, S Kumar, A Luoma, Y Ito, PH Alves da S, D Pan, JW Pyrdol, CH Yoon, KW Wucherpfen
    Cancer Immunol Res, 2020;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Lysates
    Applications: Western Blot


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