Measured by its ability to neutralize BMP‑6-induced alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. Asahina, I. et al. (1996) Exp. Cell Res. 222:38. The Neutralization Dose (ND50) is typically 1.5-7.5 μg/mL in the presence of 0.5 μg/mL Recombinant Mouse BMP‑6.
Please Note: Optimal dilutions should be determined by each laboratory for each application.
are available in the Technical Information section on our website.
Alkaline Phosphatase Production Induced by BMP-6 and Neutralization by Mouse BMP-6 Antibody.
Recombinant Mouse BMP-6 (Catalog # 6325-BM) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Alkaline Phosphatase Production elicited by Recombinant Mouse BMP‑6 (0.5 μg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse BMP‑6 Monoclonal Antibody (Catalog # MAB6325). The ND50 is typically 1.5-7.5 μg/mL.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Bone Morphogenetic Protein 6 (BMP‑6), also known as Vgr-1, is one of at least 15 structurally and functionally related BMPs which are members of the transforming growth factor beta (TGF-beta ) superfamily. Mouse BMP‑6 is synthesized as a 510 amino acid (aa) precursor protein that is cleaved at the dibasic cleavage site (RxxR) to release the 18 kDa C‑terminal mature protein. Biologically active BMP‑6 consists of a disulfide-linked homodimer of the mature proteins (1, 2). Mature mouse BMP‑6 shares 96% and 98% aa sequence identity with human and rat BMP‑6, respectively. Cellular responses to BMP‑6 are mediated by hetero-oligomeric complexes of type I (Activin RIA/ALK-2 and BMPR-IA/ALK-3) and type II (Activin RIIA and BMPR-II) serine/threonine kinase receptors (3‑5). Glycosylation of BMP‑6 is required for its interaction with Activin RIA (6). BMP‑6 induces the expression of Noggin and is subsequently antagonized by Noggin (7, 8). BMP‑6 induces a wide range of cellular responses. It promotes osteoblast differentiation from mesenchymal stem cells (5), chondrocyte maturation (9), Ang II-induced aldosterone production in the adrenal cortex (3), hormone production and responsiveness in ovarian granulosa cells (10), iNOS and TNF-alpha production in macrophages (4), the cell death of B cells (8), and neurite outgrowth (11). BMP‑6 expression is induced in astrocytes surrounding sites of brain injury where it functions as a neuroprotectant (11, 12). BMP‑6 is upregulated during prostate cancer progression and promotes tumor cell metastasis to bone (13). Through interactions with the BMP coreceptor RGM‑C/Hemojuvelin, BMP‑6 plays an important role in iron homeostasis by promoting Hepcidin expression and preventing serum iron overload (14, 15).
Chen, D. et al. (2004) Growth Factors 22:233.
Lyons, K. et al. (1989) Proc. Natl. Acad. Sci. 86:4554.
Inagaki, K. et al. (2006) Endocrinology 147:2681.
Hong, J.H. et al. (2008) Immunology 128:e442.
Lavery, K. et al. (2008) J. Biol. Chem. 283:20948.
Saremba, S. et al. (2008) FEBS J. 275:172.
Haudenschild, D.R. et al. (2004) Cancer Res. 64:8276.
Kersten, C. et al. (2005) BMC Immunol. 6:9.
Grimsrud, C.D. et al. (1999) J. Bone Miner. Res. 14:475.
Shi, J. et al. (2009) Fertil. Steril. 92:1794.
Yabe, T. et al. (2002) J. Neurosci. Res. 68:161.
Zhang, Z. et al. (2006) Neuroscience 138:47.
Dai, J. et al. (2005) Cancer Res. 65:8274.
Meynard, D. et al. (2009) Nat. Genet. 41:478.
Andriopoulos, B. Jr. et al. (2009) Nat. Genet. 41:482.
Bone Morphogenetic Protein 6
Entrez Gene IDs:
654 (Human); 12161 (Mouse); 25644 (Rat)
BMP6; BMP-6; bone morphogenetic protein 6; vegetal related growth factor (TGFB-related); vegetal-related (TGFB related) cytokine; VG-1-R; VG-1-related protein; Vg1-related sequence; VGR; VGR1; Vgr-1
Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.