Mouse C-Reactive Protein/CRP Quantikine ELISA Kit

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MCRP00
Mouse C-Reactive Protein/CRP ELISA Standard Curve
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Mouse C-Reactive Protein/CRP Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Tissue Lysates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity
0.015 ng/mL
Assay Range
0.2 - 10 ng/mL (Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant mouse CRP.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse C-Reactive Protein/CRP immunoassay is a 4.5 hour solid-phase ELISA designed to measure CRP in mouse cell culture supernates, tissue lysates, serum, and plasma. It contains NS0-expressed recombinant mouse CRP and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant mouse CRP. Results obtained using natural mouse CRP showed dose response curves that were parallel to the standard curves obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse CRP.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of kit components

Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 0.453 1.31 3.78 0.48 1.33 3.76
Standard Deviation 0.035 0.083 0.089 0.052 0.087 0.314
CV% 7.7 6.3 2.4 10.8 6.5 8.4

Recovery

The recovery of mouse CRP spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 95 82-115

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse CRP in each matrix were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Mouse C-Reactive Protein/CRP ELISA Linearity

Scientific Data

Mouse C-Reactive Protein/CRP ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: C-Reactive Protein/CRP

C-Reactive Protein (CRP), also known as Pentraxin 1, is a secreted pentameric protein that functions as a sensor and activator for the innate immune response. In humans, it is a major acute-phase protein; its circulating concentration is dramatically elevated at the onset of inflammation. In mice, however, serum CRP levels increase only slightly during inflammation, and the analogous acute phase role is filled by Pentraxin 2. CRP binds, opsonizes, and induces the phagocytosis of bacteria and apoptotic cells. It regulates activation of the classical complement pathway by binding several proteins in the complement cascade as well as Fc gamma RI, Fc gamma RIIA, and Fc gamma RIIB on macrophages and dendritic cells. It also promotes dendritic cell maturation and humoral immunity. In cardiovascular disease, CRP binds to oxidized LDL, exacerbates tissue damage in myocardial infarction, and inhibits the repair of injured vascular endothelium.

Entrez Gene IDs:
1401 (Human); 12944 (Mouse); 25419 (Rat)
Alternate Names:
C-Reactive Protein; C-reactive protein, pentraxin-related; CRP; MGC88244; pentraxin 1; PTX1MGC149895
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Assay Diluent
  4.   Add 50 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 100 µL Conjugate
  9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  10.   Aspirate and wash 4 times.

  11. 100 µL Substrate Solution
  12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 100 µL Stop Solution
  14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Mouse C-Reactive Protein/CRP Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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  1. Actions of Esomeprazole on the Maternal Vasculature in Lean and Obese Pregnant Mice with Impaired Nitric Oxide Synthesis: A Model of Preeclampsia
    Authors: N de Alwis, NK Binder, YTM Mangwiro, S Beard, N Pritchard, E Kadife, BR Fato, E Keenan, FC Brownfoot, TJ Kaitu'u-Li, NJ Hannan
    International Journal of Molecular Sciences, 2022;23(15):.
    Species: Mouse
    Sample Types: Serum
  2. Genistein attenuated oxidative stress, inflammation, and apoptosis in L-arginine induced acute pancreatitis in mice
    Authors: P Siriviriya, J Sriko, K Somanawat, M Chayanupat, N Klaikeaw, D Werawatgan
    BMC complementary medicine and therapies, 2022;22(1):208.
    Species: Mouse
    Sample Types: Serum
  3. An antibody against L1 cell adhesion molecule inhibits cardiotoxicity by regulating persistent DNA damage
    Authors: JK Nam, AR Kim, SH Choi, JH Kim, KJ Choi, S Cho, JW Lee, HJ Cho, YW Kwon, J Cho, KS Kim, J Kim, HJ Lee, TS Lee, S Bae, HJ Hong, YJ Lee
    Nature Communications, 2021;12(1):3279.
    Species: Mouse
    Sample Types: Serum
  4. Circulating Endothelial Progenitor Cells Are Preserved in Female Mice Exposed to Ambient Fine Particulate Matter Independent of Estrogen
    Authors: X Liu, Y Xiao, Q Zhu, Y Cui, H Hao, M Wang, PJ Cowan, RJ Korthuis, G Li, Q Sun, Z Liu
    International Journal of Molecular Sciences, 2021;22(13):.
    Species: Mouse
    Sample Types: Serum
  5. Pleiotropic Effects of Atorvastatin Result in a Downregulation of the Carboxypeptidase U System (CPU, TAFIa, CPB2) in a Mouse Model of Advanced Atherosclerosis
    Authors: K Claesen, L Roth, JC Mertens, K Hermans, Y Sim, D Hendriks
    Pharmaceutics, 2021;13(10):.
    Species: Mouse
    Sample Types: Plasma
  6. Pirfenidone increases IL10 and improves acute pancreatitis in multiple clinically relevant murine models
    Authors: E Palathinga, J George, M Tarique, S Iyer, P Sahay, B Gomez Agui, DB Edwards, B Giri, V Sethi, T Jain, P Sharma, U Vaish, HK C Jacob, A Ferrantell, CL Maynard, AK Saluja, RK Dawra, V Dudeja
    JCI Insight, 2021;0(0):.
    Species: Mouse
    Sample Types: Serum
  7. Inflammation-driven senescence-associated secretory phenotype in cancer-associated fibroblasts enhances peritoneal dissemination
    Authors: T Yasuda, M Koiwa, A Yonemura, K Miyake, R Kariya, S Kubota, T Yokomizo-N, N Yasuda-Yos, T Uchihara, R Itoyama, L Bu, L Fu, K Arima, D Izumi, S Iwagami, K Eto, M Iwatsuki, Y Baba, N Yoshida, H Ohguchi, S Okada, K Matsusaki, G Sashida, A Takahashi, P Tan, H Baba, T Ishimoto
    Cell Reports, 2021;34(8):108779.
    Species: Mouse
    Sample Types: Serum
  8. Monomeric C-reactive protein via endothelial CD31 for neurovascular inflammation in an ApoE genotype-dependent pattern: A risk factor for Alzheimer's disease?
    Authors: Z Zhang, H Na, Q Gan, Q Tao, Y Alekseyev, J Hu, Z Yan, JB Yang, H Tian, S Zhu, Q Li, IM Rajab, JK Blusztajn, B Wolozin, A Emili, X Zhang, T Stein, LA Potempa, WQ Qiu
    Aging Cell, 2021;0(0):e13501.
    Species: Mouse
    Sample Types: Serum
  9. Metformin treatment of high-fat diet-fed obese male mice restores sperm function and fetal growth, without requiring weight loss
    Authors: NO McPherson, M Lane
    Asian J. Androl., 2020;0(0):.
    Species: Mouse
    Sample Types: Plasma
  10. Development of insulin resistance preceded major changes in iron homeostasis in mice fed a high-fat diet
    Authors: J Varghese, JV James, R Anand, M Narayanasa, G Rebekah, B Ramakrishn, AJ Nellickal, M Jacob
    J. Nutr. Biochem., 2020;84(0):108441.
    Species: Mouse
    Sample Types: Serum
  11. Lactobacillus fermentum promotes adipose tissue oxidative phosphorylation to protect against diet-induced obesity
    Authors: Y Yoon, G Kim, MG Noh, JH Park, M Jang, S Fang, H Park
    Exp. Mol. Med., 2020;0(0):.
    Species: Mouse
    Sample Types: Serum
  12. Anti-Inflammatory and Gut Microbiota Modulatory Effect of Lactobacillus rhamnosus Strain LDTM 7511 in a Dextran Sulfate Sodium-Induced Colitis Murine Model
    Authors: S Yeo, H Park, E Seo, J Kim, BK Kim, IS Choi, CS Huh
    Microorganisms, 2020;8(6):.
    Species: Mouse
    Sample Types: Serum
  13. HIF1alpha inhibition facilitates Leflunomide-AHR-CRP signaling to attenuate bone erosion in CRP-aberrant rheumatoid arthritis
    Authors: C Liang, J Li, C Lu, D Xie, J Liu, C Zhong, X Wu, R Dai, H Zhang, D Guan, B Guo, B He, F Li, X He, W Zhang, BT Zhang, G Zhang, A Lu
    Nat Commun, 2019;10(1):4579.
    Species: Mouse
    Sample Types: Serum
  14. REDD-1 aggravates endotoxin-induced inflammation via atypical NF-?B activation
    Authors: DK Lee, JH Kim, J Kim, S Choi, M Park, W Park, S Kim, KS Lee, T Kim, J Jung, YK Choi, KS Ha, MH Won, TR Billiar, YG Kwon, YM Kim
    FASEB J., 2018;0(0):fj201701436R.
    Species: Mouse
    Sample Types: Serum
  15. Berberine inhibits the ischemia-reperfusion injury induced inflammatory response and apoptosis of myocardial cells through the phosphoinositide 3-kinase/RAC-? serine/threonine-protein kinase and nuclear factor-?B signaling pathways
    Authors: L Wang, H Ma, Y Xue, H Shi, T Ma, X Cui
    Exp Ther Med, 2018;15(2):1225-1232.
    Species: Mouse
    Sample Types: Serum

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