Detection of C1q R1/CD93 in M1 Mouse Cell Line by Flow Cytometry. M1 mouse myeloid leukemia cell line was stained with Mouse C1q R1/CD93 Monoclonal Antibody (Catalog # MAB1696, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')2 Secondary Antibody (Catalog # F0113).
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: C1q R1/CD93
Complement component 1, q subcomponent receptor (C1q R1), also known as C1q Rp, Collectin receptor and AA4 antigen in rodents, mediates enhanced phagocytosis by monocytes and macrophages upon interaction with soluble defense collagens including C1q, MBL (mannose-binding lectin) and SP-A (pulmonary surfactant protein A) (1, 2). It is a type I transmembrane glycoprotein expressed on endothelial cells, hematopoietic progenitor cells, platelets and cells of myeloid origin. C1q R1 has also been identified as a stem cell marker (3, 4).
Kim, T.S. et al. (2000) Mol. Immunol. 37:377.
Dean, Y.D. et al. (2001) Eur. J. Immunol. 31:1370.
Petrenko, O. et al. (1999) Immunity 10:691.
Danet, G.H. et al. (2002) Proc. Natl. Acad. Sci. USA 99:1044.
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but also provides information about sample types, species, and experimental conditions.
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