Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry, Immunocytochemistry, CyTOF-ready

Cited:

Immunohistochemistry-Paraffin, CyTof, ELISA Development

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 223437
View all C1qR1/CD93 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse C1q R1/CD93
Ala23-Asn572
Accession # O89103

Specificity

Detects mouse C1q R1/CD93 in direct ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse C1qR1/CD93 Antibody

Detection of C1q R1/CD93 antibody in M1 Mouse Cell Line antibody by Flow Cytometry.

Detection of C1q R1/CD93 in M1 Mouse Cell Line by Flow Cytometry.

M1 mouse myeloid leukemia cell line was stained with Mouse C1q R1/CD93 Monoclonal Antibody (Catalog # MAB1696, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (Catalog # F0113).

Applications for Mouse C1qR1/CD93 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: M1 mouse myeloid leukemia cell line

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed M1 mouse myeloid leukemia cell line

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: C1qR1/CD93

Complement component 1, q subcomponent receptor (C1q R1), also known as C1q Rp, Collectin receptor and AA4 antigen in rodents, mediates enhanced phagocytosis by monocytes and macrophages upon interaction with soluble defense collagens including C1q, MBL (mannose-binding lectin) and SP-A (pulmonary surfactant protein A) (1, 2). It is a type I transmembrane glycoprotein expressed on endothelial cells, hematopoietic progenitor cells, platelets and cells of myeloid origin. C1q R1 has also been identified as a stem cell marker (3, 4).

References

  1. Kim, T.S. et al. (2000) Mol. Immunol. 37:377.
  2. Dean, Y.D. et al. (2001) Eur. J. Immunol. 31:1370.
  3. Petrenko, O. et al. (1999) Immunity 10:691.
  4. Danet, G.H. et al. (2002) Proc. Natl. Acad. Sci. USA 99:1044.

Long Name

Complement Component C1q Receptor

Alternate Names

AA4 Antigen, C1q R1, C1q Rp, C1qRp, CD93, Collectin Receptor, Ly68

Entrez Gene IDs

22918 (Human); 17064 (Mouse); 84398 (Rat)

Gene Symbol

CD93

UniProt

O89103

Additional C1qR1/CD93 Products

Product Documents for Mouse C1qR1/CD93 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse C1qR1/CD93 Antibody

For research use only

Citations for Mouse C1qR1/CD93 Antibody

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Protocols

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