Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 336103
View all Carbonic Anhydrase XII/CA12 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Carbonic Anhydrase XII/CA12
Ala25-Ser301
Accession # Q8CI85

Specificity

Detects mouse Carbonic Anhydrase XII/CA12 in direct ELISAs and Western blots. In Western blots, 100% cross‑reactivity with recombinant human (rh) CA12, 10% cross-reactivity with rhCA2, and no cross-reactivity with rhCA1, 3, 5A, 5B, 6, 7, 8, 10, 11, 13, recombinant mouse CA4, 9, or 14 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Mouse Carbonic Anhydrase XII/CA12 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse Carbonic Anhydrase XII (Catalog # 2345-CA)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Carbonic Anhydrase XII/CA12

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Mouse CA12 is a type I membrane enzyme with both CA activity and esterase activity (2, 3). The amino acid sequence of mouse CA12 shares 97%, 82%, and 68% identity with that of rat, human/chimpanzee/canine, and chicken.

References

  1. Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
  2. Strausberg, R.L. et al. (2002) Proc. Natl. Acad. Sci. USA 99:16899.
  3. Okazaki, Y. et al. (2002) Nature 420:563.

Alternate Names

CA12

Entrez Gene IDs

771 (Human); 76459 (Mouse)

Gene Symbol

CA12

UniProt

Q8CI85

Additional Carbonic Anhydrase XII/CA12 Products

Product Documents for Mouse Carbonic Anhydrase XII/CA12 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Carbonic Anhydrase XII/CA12 Antibody

For research use only

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