Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 186513
View all Cathepsin E Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Cathepsin E
Gln19-Pro397
Accession # P70269

Specificity

Detects mouse Cathepsin E in direct ELISAs and Western blots. It recognizes both the pro and active forms of recombinant mouse (rm) Cathepsin E. In Western blots, no cross-reactivity with rmCathepsin B, C, H, L, or recombinant human Cathepsin O, S, or V is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Mouse Cathepsin E Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse Cathepsin E (Catalog # 1130-AS)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cathepsin E

Cathepsin E is an intracellular aspartic protease of the pepsin family (1, 2). Unlike Cathepsin D, another member of the same family and a lysosomal protease with relatively ubiquitous distribution, Cathepsin E is not a lysosomal enzyme and has a limited cell and tissue distribution. However, both Cathepsins D and E play an important role in the degradation of proteins, the generation of bioactive proteins, and antigen processing (3). Both enzymes are efficient in cleaving the Swedish mutant of amyloid precursor protein (APP) at the beta site but show almost no reactivity with the wild-type APP (4). Mouse Cathepsin E is synthesized as a precursor protein, consisting of a signal peptide (residues 1-18), a propeptide (residues 19-59), and a mature chain (residues 60-397) (1).

References

  1. Tatnell, P.J. et al. (1997) FEBS Lett. 408:62.
  2. Kay, J. and P.J. Tatnell (2004) in Handbook of Proteolytic Enzymes (Barrett, A.J. et al., eds.), p. 33,  Academic Press, San Diego.
  3. Tsukuba, T. et al. (2000) Mol. Cells 10:601.
  4. Gruninger-Leitch, F. et al. (2000) Nat. Biotechnol. 18:66.

Alternate Names

CTSE

Entrez Gene IDs

1510 (Human); 13034 (Mouse)

Gene Symbol

CTSE

UniProt

P70269

Additional Cathepsin E Products

Product Documents for Mouse Cathepsin E Antibody

Certificate of Analysis

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Product Specific Notices for Mouse Cathepsin E Antibody

For research use only

Citations for Mouse Cathepsin E Antibody

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