Mouse CCL11/Eotaxin Antibody

(17 citations)
(1 Review)
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CCL11/Eotaxin in ELISAs and Western blots. In Western blots, approximately 50% cross-reactivity with recombinant human CCL11/Eotaxin and recombinant mouse (rm) MARC is observed, and less than 2% cross-reactivity with rmJE is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant mouse CCL11/Eotaxin
    His24-Pro97
    Accession # P48298
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse CCL11/Eotaxin (Catalog # 420-ME)
  • Immunohistochemistry
    5-15 µg/mL
    See below
    • Mouse CCL11/Eotaxin Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    0.2-0.8 µg/mL 
    Mouse CCL11/Eotaxin Antibody (Catalog # AF-420-NA)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Mouse CCL11/Eotaxin Biotinylated Antibody (Catalog # BAF420)
  • ELISA Standard
     
    Recombinant Mouse CCL11/Eotaxin Protein (Catalog # 420-ME)
  • Neutralization
    Measured by its ability to neutralize CCL11/Eotaxin-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CCR3. The Neutralization Dose (ND50) is typically 0.1-0.5 µg/mL in the presence of 10 ng/mL Recombinant Mouse CCL11/Eotaxin.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Chemotaxis Induced by CCL11/Eotaxin and Neutral­ization by Mouse CCL11/
Eotaxin Antibody.
Recombinant Mouse CCL11/
Eotaxin (Catalog # 420‑ME) chemoattracts the BaF3 mouse pro‑B cell line transfected with mouse CCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL11/
Eotaxin (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL11/Eotaxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-420-NA). The ND50 is typically
0.1‑0.5 µg/mL.

CCL11/Eotaxin in Mouse Thymus. CCL11/Eotaxin was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse CCL11/Eotaxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-420-NA) at 5 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Immunohistochemistry
CCL11/Eotaxin in Mouse Colon. CCL11/Eotaxin was detected in perfusion fixed frozen sections of mouse colon using Goat Anti-Mouse CCL11/Eotaxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-420-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL11/Eotaxin

CCL11 is a potent eosinophil chemoattractant that was originally purified from bronchoalveolar lavage fluid of guinea pigs sensitized by aerosol challenge with ovalbumin. Microsequencing of the purified protein revealed the guinea pig CCL11 to be a member of the beta (CC) chemokine family of inflammatory and immunoregulatory cytokines. cDNA clones for guinea pig, mouse and human CCL11 have been isolated. Mouse CCL11 cDNA encodes a 97 amino acid residue precursor protein from which the amino-terminal 23 amino acid residues are cleaved to generate the 74 amino acid residue mature mouse CCL11. At the protein sequence level, mature mouse CCL11 is approximately 60% identical to mature human and guinea pig CCL11. In addition, mouse CCL11 also shows high amino acid sequence identity to members of the MCP family. Mouse CCL11 is chemotactic for eosinophils, but not mononuclear cells or neutrophils. CCL11 mRNA is expressed in a variety of tissues. The expression of CCL11 mRNA is induced in cultured endothelial cells in response to IFN-gamma. In addition, CCL11 mRNA is also induced in response to the transplantation of IL-4-secreting tumor cells. The CC chemokine receptor 3 (CCR3) has now been identified to be a specific human CCL11 receptor.

  • References:
    1. Rothenberg, M.E. et al. (1995) Proc. Natl. Acad. Sci. USA 92:8960.
    2. Kitaura, M. et al. (1996) J. Biol. Chem 271:7725.
    3. Garcia-Zepeda, E.A. et al. (1996) Nature Medicine 2:449.
    4. Ponath, P.D. et al. (1996) J. Clin. Invest. 97:604.
  • Entrez Gene IDs:
    6356 (Human); 20292 (Mouse)
  • Alternate Names:
    C-C motif chemokine 11; CCL11; chemokine (C-C motif) ligand 11; Eosinophil chemotactic protein; Eotaxin; eotaxin-1; MGC22554; SCYA11Small-inducible cytokine A11; small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin)
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. CCL11, a novel mediator of inflammatory bone resorption
    Authors: E Kindstedt, CK Holm, R Sulniute, I Martinez-C, R Lundmark, P Lundberg
    Sci Rep, 2017;7(1):5334.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  2. Macrophages and cardiac fibroblasts are the main producers of eotaxins and regulate eosinophil trafficking to the heart
    Eur J Immunol, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Paraffin embedded
  3. Iddm30 controls pancreatic expression of Ccl11 (Eotaxin) and the Th1/Th2 balance within the insulitic lesions.
    Authors: Chao G, Wallis R, Marandi L, Ning T, Sarmiento J, Paterson A, Poussier P
    J Immunol, 2014;192(8):3645-53.
    Species: Rat
    Sample Type: Cell Lysates
    Application: WB
  4. Suppression of laser-induced choroidal neovascularization by a CCR3 antagonist.
    Authors: Mizutani, Takeshi, Ashikari, Masayuki, Tokoro, Mayumi, Nozaki, Miho, Ogura, Yuichiro
    Invest Ophthalmol Vis Sci, 2013;54(2):1564-72.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC OCT-embedded
  5. Response patterns of cytokines/chemokines in two murine strains after irradiation.
    Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
    Cytokine, 2012;58(2):169-77.
    Species: Mouse
    Sample Type: Plasma
    Application: Luminex Assay Development
  6. The ageing systemic milieu negatively regulates neurogenesis and cognitive function.
    Authors: Villeda SA, Luo J, Mosher KI, Zou B, Britschgi M, Bieri G, Stan TM, Fainberg N, Ding Z, Eggel A, Lucin KM, Czirr E, Park JS, Couillard-Despres S, Aigner L, Li G, Peskind ER, Kaye JA, Quinn JF, Galasko DR, Xie XS, Rando TA, Wyss-Coray T
    Nature, 2011;477(7362):90-4.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  7. House dust mite induces direct airway inflammation in vivo: implications for future disease therapy?
    Authors: De Alba J, Raemdonck K, Dekkak A, Collins M, Wong S, Nials AT, Knowles RG, Belvisi MG, Birrell MA
    Eur. Respir. J., 2010;35(6):1377-87.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  8. Comprehensive assessment of chemokine expression profiles by flow cytometry.
    Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
    J. Clin. Invest., 2010;120(3):907-23.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  9. CCR3 is a target for age-related macular degeneration diagnosis and therapy.
    Authors: Takeda A, Baffi JZ, Kleinman ME, Cho WG, Nozaki M, Yamada K, Kaneko H, Albuquerque RJ, Dridi S, Saito K, Raisler BJ, Budd SJ, Geisen P, Munitz A, Ambati BK, Green MG, Ishibashi T, Wright JD, Humbles AA, Gerard CJ, Ogura Y, Pan Y, Smith JR, Grisanti S, Hartnett ME, Rothenberg ME, Ambati J
    Nature, 2009;460(7252):225-30.
    Species: Mouse
    Sample Type: Vitreous Humor
    Application: Neut
  10. Resolution of allergic inflammation and airway hyperreactivity is dependent upon disruption of the T1/ST2-IL-33 pathway.
    Authors: Kearley J, Buckland KF, Mathie SA, Lloyd CM
    Am. J. Respir. Crit. Care Med., 2009;179(9):772-81.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  11. Toll-like receptor-2-mediated C-C chemokine receptor 3 and eotaxin-driven eosinophil influx induced by Mycobacterium bovis BCG pleurisy.
    Authors: D&amp;amp;apos;Avila H, Almeida PE, Roque NR, Castro-Faria-Neto HC, Bozza PT
    Infect. Immun., 2007;75(3):1507-11.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  12. IL-13Ralpha2 and IL-10 coordinately suppress airway inflammation, airway-hyperreactivity, and fibrosis in mice.
    Authors: Wilson MS, Elnekave E, Mentink-Kane MM, Hodges MG, Pesce JT, Ramalingam TR, Thompson RW, Kamanaka M, Flavell RA, Keane-Myers A, Cheever AW, Wynn TA
    J. Clin. Invest., 2007;117(10):2941-51.
    Species: Mouse
    Sample Type: Serum
  13. Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality.
    Authors: Osuchowski MF, Welch K, Siddiqui J, Remick DG
    J. Immunol., 2006;177(3):1967-74.
    Species: Mouse
    Sample Type: Plasma
    Application: ELISA Development
  14. Sequential ELISA to profile multiple cytokines from small volumes.
    Authors: Osuchowski MF, Siddiqui J, Copeland S, Remick DG
    J. Immunol. Methods, 2005;302(1):172-81.
    Species: Mouse
    Sample Type: Plasma
    Application: ELISA Development
  15. A soluble chemokine-binding protein from vaccinia virus reduces virus virulence and the inflammatory response to infection.
    Authors: Reading PC, Symons JA, Smith GL
    J. Immunol., 2003;170(3):1435-42.
    Species: Human
    Sample Type: BALF
    Application: ELISA Development
  16. P-selectin mediates IL-13-induced eosinophil transmigration but not eotaxin generation in vivo: a comparative study with IL-4-elicited responses.
    Authors: Larbi KY, Dangerfield JP, Culley FJ, Marshall D, Haskard DO, Jose PJ, Williams TJ, Nourshargh S
    J. Leukoc. Biol., 2003;73(1):65-73.
    Species: Mouse
    Sample Type: Peritoneal Fluid
    Application: ELISA Development
  17. AMD3100, a CxCR4 antagonist, attenuates allergic lung inflammation and airway hyperreactivity.
    Authors: Lukacs NW, Berlin A, Schols D, Skerlj RT, Bridger GJ
    Am. J. Pathol., 2002;160(4):1353-60.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: ELISA Development
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Average Rating: 4 (Based on 1 review)

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Western Blot Mouse CCL11/Eotaxin Antibody AF-420-NA
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Western Blot Mouse CCL11/Eotaxin Antibody AF-420-NA
Western Blot: Mouse CCL11/Eotaxin Antibody [AF-420-NA]

Summary

ApplicationWestern Blot
Sample TestedRat pancreatic islet lysate

Other Experimental Details

Other Experimental DetailsRat pancreatic islet lysate were blotted overnight in 4 degree with polyclonal goat anti-mouse Ccl11. Serial dilution of lysate in the firs 2 lanes followed by 2 lanes of recombinant rat Ccl11.
Specificity: Reasonably specific
Sensitivity: Reasonably sensitive
Buffer: PBS
Dilution: 1/1000

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