Mouse CCL17/TARC Antibody

(11 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CCL17/TARC in ELISAs.
  • Source
    Monoclonal Rat IgG2A Clone # 110904
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived Recombinant Mouse CCL17/TARC
    Ala24-Pro93
    Accession # Q9WUZ6
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
    • Mouse CCL17/TARC Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Mouse CCL17/TARC Antibody (Catalog # MAB529)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Mouse CCL17/TARC Biotinylated Antibody (Catalog # BAF529)
  • ELISA Standard
     
    Recombinant Mouse CCL17/TARC Protein (Catalog # 529-TR)
  • Neutralization
    Measured by its ability to neutralize CCL17/TARC-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR4. The Neutralization Dose (ND50) is typically 0.75-3.0 µg/mL in the presence of 20 ng/mL Recombinant Mouse CCL17/TARC.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Chemotaxis Induced by CCL17/TARC and Neutralization by Mouse CCL17/TARC Antibody. Recombinant Mouse CCL17/TARC (Catalog # 529-TR) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL17/TARC (20 ng/mL) is neutralized (green line) by increasing concentrations of Mouse CCL17/TARC Monoclonal Antibody (Catalog # MAB529). The ND50 is typically 0.75-3.0 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL17/TARC

Human thymus and activation-regulated chemokine (TARC) also known as CCL17, is a novel CC chemokine identified using a signal sequence trap method. Mouse CCL17 was discovered as a dendritic cell (DC) specific gene by differentiation RNA display. Mouse CCL17 cDNA encodes a highly basic 93 amino acid (aa) residue precursor protein with a 23 aa residue putative signal peptide that is cleaved to generate the 70 aa residue mature secreted protein. Among CC chemokine family members, CCL17 has approximately 24-29% amino acid sequence identity with RANTES, MIP-1 alpha, MIP-1 beta, MCP-1, MCP-2, MCP-3, and I-309. The gene for human CCL17 has been mapped to chromosome 16q13 rather than chromosome 17 where the genes for many human CC chemokines are clustered. Mouse CCL17 is constitutively expressed in thymic DC, and at a lower level in lymph node DC in the lung. Recombinant CCL17 has been shown to be chemotactic for T cell lines and antigen-primed T helper cells. In humans, CCL17 was identified to be a specific functional ligand for CCR4 and CCR8, receptors that are selectively expressed on T cells.

  • References:
    1. Imai, T. et al. (1997) J. Biol. Chem. 272:15036.
    2. Imai, T. et al. (1996) J. Biol. Chem. 271:21514.
    3. Nomiyama, H. et al. (1997) Genomics 40:211.
    4. Lieberam, I. et al. (1999) Eur. J. Immunol. 29:2684.
  • Entrez Gene IDs:
    6361 (Human); 20295 (Mouse)
  • Alternate Names:
    ABCD-2; CC chemokine TARC; C-C motif chemokine 17; CCL17; chemokine (C-C motif) ligand 17; MGC138273; SCYA17MGC138271; small inducible cytokine subfamily A (Cys-Cys), member 17; Small-inducible cytokine A17; T cell-directed CC chemokine; TARC; TARCA-152E5.3; Thymus and activation-regulated chemokine
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. iNKT Cell Emigration out of the Lung Vasculature Requires Neutrophils and Monocyte-Derived Dendritic Cells in Inflammation
    Cell Rep, 2016;16(12):3260-72.
  2. Granulocyte macrophage colony-stimulating factor induces CCL17 production via IRF4 to mediate inflammation
    J Clin Invest, 2016;0(0):.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  3. Engagement of two distinct binding domains on CCL17 is required for signaling through CCR4 and establishment of localized inflammatory conditions in the lung.
    Authors: Santulli-Marotto S, Boakye K, Lacy E, Wu S, Luongo J, Kavalkovich K, Coelho A, Hogaboam C, Ryan M
    PLoS ONE, 2013;8(12):e81465.
    Species: Mouse
    Sample Type: Recombinant Protein
    Application: DirELISA
  4. Response patterns of cytokines/chemokines in two murine strains after irradiation.
    Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
    Cytokine, 2012;58(2):169-77.
    Species: Mouse
    Sample Type: Plasma
    Application: Luminex Assay Development
  5. CCL17 controls mast cells for the defense against filarial larval entry.
    Authors: Specht S, Frank JK, Alferink J, Dubben B, Layland LE, Denece G, Bain O, Forster I, Kirschning CJ, Martin C, Hoerauf A
    J. Immunol., 2011;186(8):4845-52.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  6. Resolution of allergic inflammation and airway hyperreactivity is dependent upon disruption of the T1/ST2-IL-33 pathway.
    Authors: Kearley J, Buckland KF, Mathie SA, Lloyd CM
    Am. J. Respir. Crit. Care Med., 2009;179(9):772-81.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  7. IL-12 produced by dendritic cells augments CD8+ T cell activation through the production of the chemokines CCL1 and CCL17.
    Authors: Henry CJ, Ornelles DA, Mitchell LM, Brzoza-Lewis KL, Hiltbold EM
    J. Immunol., 2008;181(12):8576-84.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  8. CD4+CD25+ regulatory T cells reverse established allergic airway inflammation and prevent airway remodeling.
    Authors: Kearley J, Robinson DS, Lloyd CM
    J. Allergy Clin. Immunol., 2008;122(3):617-24.e6.
    Species: Mouse
    Sample Type: BALF
    Application: ELISA Development
  9. iNKT cells require CCR4 to localize to the airways and to induce airway hyperreactivity.
    Authors: Meyer EH, Wurbel MA, Staton TL, Pichavant M, Kan MJ, Savage PB, DeKruyff RH, Butcher EC, Campbell JJ, Umetsu DT
    J. Immunol., 2007;179(7):4661-71.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  10. The direct action of 1alpha,25(OH)2-vitamin D3 on purified mouse Langerhans cells.
    Authors: Fujita H, Asahina A, Komine M, Tamaki K
    Cell. Immunol., 2007;245(2):70-9.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  11. 17beta-estradiol (E2) modulates cytokine and chemokine expression in human monocyte-derived dendritic cells.
    Authors: Bengtsson AK, Ryan EJ, Giordano D, Magaletti DM, Clark EA
    Blood, 2004;104(5):1404-10.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
Expand to show all 11 Citations
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