Mouse CCL17/TARC Quantikine ELISA Kit

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MCC170
Mouse CCL17/TARC ELISA Standard Curve
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Product Details
Procedure
Citations (36)
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Mouse CCL17/TARC Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL)
Sensitivity
5 pg/mL
Assay Range
7.8 - 500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma)
Specificity
Natural and recombinant mouse TARC
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse TARC Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse TARC levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse TARC and antibodies raised against the recombinant factor. Results obtained for naturally occurring mouse TARC showed linear curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse TARC.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 27 89 278 29 98 310
Standard Deviation 1.3 2.3 7.8 2.2 6.9 17.3
CV% 4.8 2.6 2.8 7.6 7 5.6

Recovery

The recovery of mouse TARC spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=6) 94 85-113
EDTA Plasma (n=6) 94 83-109
Serum (n=6) 94 82-103

Linearity

To assess the linearity of the assay, five or more samples containing and/or spiked with various concentrations of mouse TARC in each matrix were diluted with Calibrator Diluent and assayed.
Mouse CCL17/TARC ELISA Linearity

Data Examples

Mouse CCL17/TARC ELISA Standard Curve

Product Datasheets

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CCL17/TARC

CCL17/TARC is a chemokine that interacts with CCR4 and CCR8 to induce the chemoattraction of activated Th2 cells, basophils, and NK cells. It is produced by thymic dendritic cells, lymph node dendritic cells, monocytes, CD4+ T cells, keratinocytes, fibroblasts, bronchial epithelial cells, and Reed-Sternberg cells. CCL17 promotes Th2 cell recruitment to sites of inflammation and induces platelet aggregation and degranulation.

Entrez Gene IDs:
6361 (Human); 20295 (Mouse)
Alternate Names:
ABCD-2; CC chemokine TARC; C-C motif chemokine 17; CCL17; chemokine (C-C motif) ligand 17; MGC138273; SCYA17; SCYA17MGC138271; small inducible cytokine subfamily A (Cys-Cys), member 17; Small-inducible cytokine A17; T cell-directed CC chemokine; TARC; TARCA-152E5.3; Thymus and activation-regulated chemokine
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Assay Diluent
  4.   Add 50 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

  8. 100 µL Conjugate
  9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  10.   Aspirate and wash 5 times.

  11. 100 µL Substrate Solution
  12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 100 µL Stop Solution
  14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Mouse CCL17/TARC Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

36 Citations: Showing 1 - 10
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  1. Airway inflammation after epicutaneous sensitization of mice requires protease activity of low-dose allergen inhalation
    Authors: I Nishioka, T Takai, N Maruyama, S Kamijo, P Suchiva, M Suzuki, S Kunimine, H Ochi, S Shimura, K Sudo, H Ogawa, K Okumura, S Ikeda
    J. Allergy Clin. Immunol., 2018;0(0):.
    Species: Mouse
    Sample Types: BALF
  2. Sox7 promotes high-grade glioma by increasing VEGFR2-mediated vascular abnormality
    Authors: IK Kim, K Kim, E Lee, DS Oh, CS Park, S Park, JM Yang, JH Kim, HS Kim, DT Shima, JH Kim, SH Hong, YH Cho, YH Kim, JB Park, GY Koh, YS Ju, HK Lee, S Lee, I Kim
    J. Exp. Med., 2018;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Capture
  3. Intratumoral IFN-? gene delivery reduces tumor-infiltrating regulatory T cells through the downregulation of tumor CCL17 expression
    Authors: A Hirata, H Hashimoto, C Shibasaki, K Narumi, K Aoki
    Cancer Gene Ther., 2018;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  4. Eosinophil recruitment is dynamically regulated by interplay among lung dendritic cell subsets after allergen challenge
    Authors: S Yi, J Zhai, R Niu, G Zhu, M Wang, J Liu, H Huang, Y Wang, X Jing, L Kang, W Song, Y Shi, H Tang
    Nat Commun, 2018;9(1):3879.
    Species: Mouse
    Sample Types: BALF
  5. Dysbiosis and Staphylococcus aureus Colonization Drives Inflammation in Atopic Dermatitis.
    Authors: Kobayashi T, Glatz M, Horiuchi K, Kawasaki H, Akiyama H, Kaplan D, Kong H, Amagai M, Nagao K
    Immunity, 2015;42(4):756-66.
    Species: Mouse
    Sample Types: Serum
  6. Dendritic cells induce Th2-mediated airway inflammatory responses to house dust mite via DNA-dependent protein kinase.
    Authors: Mishra, Amarjit, Brown, Alexandr, Yao, Xianglan, Yang, Shutong, Park, Sung-Jun, Liu, Chengyu, Dagur, Pradeep, McCoy, J Philip, Keeran, Karen J, Nugent, Gayle Z, Jeffries, Kenneth, Qu, Xuan, Yu, Zu-Xi, Levine, Stewart, Chung, Jay H
    Nat Commun, 2015;6(0):6224.
    Species: Mouse
    Sample Types: BALF
  7. Cleavage of the T cell protein tyrosine phosphatase by the hepatitis C virus nonstructural 3/4A protease induces a Th1 to Th2 shift reversible by ribavirin therapy.
    Authors: Brenndorfer E, Brass A, Karthe J, Ahlen G, Bode J, Sallberg M
    J Immunol, 2014;192(4):1671-80.
    Species: Mouse
    Sample Types: Cell Lysates
  8. Hypomorphic mutation in the RAG2 gene affects dendritic cell distribution and migration.
    Authors: Maina, Virginia, Marrella, Veronica, Mantero, Stefano, Cassani, Barbara, Fontana, Elena, Anselmo, Achille, Del Prete, Annalisa, Sozzani, Silvano, Vezzoni, Paolo, Poliani, Pietro L, Villa, Anna
    J Leukoc Biol, 2013;94(6):1221-30.
    Species: Mouse
    Sample Types: Tissue Homogenates
  9. Dual functions of prostaglandin D2 in murine contact hypersensitivity via DP and CRTH2.
    Authors: Yamamoto Y, Otani S, Hirai H, Nagata K, Aritake K, Urade Y, Narumiya S, Yokozeki H, Nakamura M, Satoh T
    Am. J. Pathol., 2012;179(1):302-14.
    Species: Mouse
    Sample Types: Tissue Homogenates
  10. Chitin elicits CCL2 from airway epithelial cells and induces CCR2-dependent innate allergic inflammation in the lung.
    J. Immunol., 2012;189(5):2545-52.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  11. IL-22 Is Produced by Innate Lymphoid Cells and Limits Inflammation in Allergic Airway Disease.
    Authors: Taube C, Tertilt C, Gyulveszi G, Dehzad N, Kreymborg K, Schneeweiss K, Michel E, Reuter S, Renauld JC, Arnold-Schild D, Schild H, Buhl R, Becher B
    PLoS ONE, 2011;6(7):e21799.
    Species: Mouse
    Sample Types: BALF
  12. Reciprocal expression of IL-25 and IL-17A is important for allergic airways hyperreactivity.
    Authors: Barlow JL, Flynn RJ, Ballantyne SJ, McKenzie AN
    Clin. Exp. Allergy, 2011;41(10):1447-55.
    Species: Mouse
    Sample Types: BALF
  13. Thymic stromal lymphopoietin is produced by dendritic cells.
    Authors: Kashyap M, Rochman Y, Spolski R
    J. Immunol., 2011;187(3):1207-11.
    Species: Mouse
    Sample Types: BALF
  14. IL-33 and M2a alveolar macrophages promote lung defense against the atypical fungal pathogen Pneumocystis murina.
    Authors: Nelson MP, Christmann BS, Werner JL, Metz AE, Trevor JL, Lowell CA, Steele C
    J. Immunol., 2011;186(4):2372-81.
    Species: Mouse
    Sample Types: Tissue Homogenates
  15. IL-33-activated dendritic cells are critical for allergic airway inflammation.
    Authors: Besnard AG, Togbe D, Guillou N, Erard F, Quesniaux V, Ryffel B
    Eur. J. Immunol., 2011;41(6):1675-86.
    Species: Mouse
    Sample Types: BALF
  16. Endogenous IL-33 enhances Th2 cytokine production and T-cell responses during allergic airway inflammation.
    Authors: Louten J, Rankin AL, Li Y
    Int. Immunol., 2011;23(5):307-15.
    Species: Mouse
    Sample Types: BALF
  17. Thymic stromal lymphopoietin is a key mediator of breast cancer progression.
    Authors: Olkhanud PB, Rochman Y, Bodogai M
    J. Immunol., 2011;186(10):5656-62.
    Species: Mouse
    Sample Types: Plasma
  18. Murine atopic dermatitis responds to peroxisome proliferator-activated receptors alpha and beta/delta (but not gamma) and liver X receptor activators.
    Authors: Hatano Y, Man MQ, Uchida Y, Crumrine D, Mauro TM, Feingold KR, Elias PM, Holleran WM
    J. Allergy Clin. Immunol., 2010;125(1):160-9.e1-5.
    Species: Mouse
    Sample Types: Serum
  19. Foxa2 programs Th2 cell-mediated innate immunity in the developing lung.
    Authors: Chen G, Wan H, Luo F
    J. Immunol., 2010;184(11):6133-41.
    Species: Mouse
    Sample Types: BALF
  20. Maintenance of an acidic stratum corneum prevents emergence of murine atopic dermatitis.
    Authors: Hatano Y, Man MQ, Uchida Y, Crumrine D, Scharschmidt TC, Kim EG, Mauro TM, Feingold KR, Elias PM, Holleran WM
    J. Invest. Dermatol., 2009;129(7):1824-35.
    Species: Mouse
    Sample Types: Serum
  21. Lovastatin inhibits antigen-induced airway eosinophilia without affecting the production of inflammatory mediators in mice.
    Authors: Chiba Y, Sato S, Misawa M
    Inflamm. Res., 2009;0(0):.
    Species: Mouse
    Sample Types: BALF
  22. Disruption of interleukin-1 signaling improves the quality of wound healing.
    Authors: Thomay AA, Daley JM, Sabo E, Worth PJ, Shelton LJ, Harty MW, Reichner JS, Albina JE
    Am. J. Pathol., 2009;174(6):2129-36.
    Species: Mouse
    Sample Types: Tissue Secretion
  23. CD11b+ myeloid cells are the key mediators of Th2 cell homing into the airway in allergic inflammation.
    Authors: Medoff BD, Seung E, Hong S, Thomas SY, Sandall BP, Duffield JS, Kuperman DA, Erle DJ, Luster AD
    J. Immunol., 2009;182(1):623-35.
    Species: Mouse
    Sample Types: BALF
  24. Cyclooxygenase inhibition during allergic sensitization increases STAT6-independent primary and memory Th2 responses.
    Authors: Zhou W, Newcomb DC, Moore ML, Goleniewska K, O'Neal JF, Peebles RS
    J. Immunol., 2008;181(8):5360-7.
    Species: Mouse
    Sample Types: Tissue Homogenates
  25. An eosinophil immune response characterizes the inflammatory skin disease observed in Tie-2 transgenic mice.
    Authors: Voskas D, Babichev Y, Ling LS, Alami J, Shaked Y, Kerbel RS, Ciruna B, Dumont DJ
    J. Leukoc. Biol., 2008;84(1):59-67.
    Species: Mouse
    Sample Types: Serum
  26. Allergic pulmonary inflammation in mice is dependent on eosinophil-induced recruitment of effector T cells.
    Authors: Jacobsen EA, Ochkur SI, Pero RS, Taranova AG, Protheroe CA, Colbert DC, Lee NA, Lee JJ
    J. Exp. Med., 2008;205(3):699-710.
    Species: Mouse
    Sample Types: BALF
  27. A novel mechanism for CCR4 in the regulation of macrophage activation in bleomycin-induced pulmonary fibrosis.
    Authors: Trujillo G, O'Connor EC, Kunkel SL, Hogaboam CM
    Am. J. Pathol., 2008;172(5):1209-21.
    Species: Mouse
    Sample Types: Tissue Homogenates
  28. iNKT cells require CCR4 to localize to the airways and to induce airway hyperreactivity.
    Authors: Meyer EH, Wurbel MA, Staton TL, Pichavant M, Kan MJ, Savage PB, DeKruyff RH, Butcher EC, Campbell JJ, Umetsu DT
    J. Immunol., 2007;179(7):4661-71.
    Species: Mouse
    Sample Types: Tissue Homogenates
  29. Prolonged inhaled allergen exposure can induce persistent tolerance.
    Authors: Van Hove CL, Maes T, Joos GF, Tournoy KG
    Am. J. Respir. Cell Mol. Biol., 2007;36(5):573-84.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  30. Therapeutic targeting of CC ligand 21 or CC chemokine receptor 7 abrogates pulmonary fibrosis induced by the adoptive transfer of human pulmonary fibroblasts to immunodeficient mice.
    Authors: Pierce EM, Carpenter K, Jakubzick C, Kunkel SL, Flaherty KR, Martinez FJ, Hogaboam CM
    Am. J. Pathol., 2007;170(4):1152-64.
    Species: Mouse
    Sample Types: Tissue Homogenates
  31. Airway epithelial STAT3 is required for allergic inflammation in a murine model of asthma.
    Authors: Simeone-Penney MC, Severgnini M, Tu P, Homer RJ, Mariani TJ, Cohn L, Simon AR
    J. Immunol., 2007;178(10):6191-9.
    Species: Mouse
    Sample Types: BALF
  32. The direct action of 1alpha,25(OH)2-vitamin D3 on purified mouse Langerhans cells.
    Authors: Fujita H, Asahina A, Komine M, Tamaki K
    Cell. Immunol., 2007;245(2):70-9.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  33. CCL17 transgenic mice show an enhanced Th2-type response to both allergic and non-allergic stimuli.
    Authors: Tsunemi Y, Saeki H, Nakamura K, Nagakubo D, Nakayama T, Yoshie O, Kagami S, Shimazu K, Kadono T, Sugaya M, Komine M, Matsushima K, Tamaki K
    Eur. J. Immunol., 2006;36(8):2116-27.
    Species: Mouse
    Sample Types: Serum
  34. Local production of chemokines and prostaglandin E2 in the acute, chronic and recovery phase of murine experimental colitis.
    Authors: Drmotova M, Rehnstrom E, Jansson L
    Cytokine, 2006;35(5):275-83.
    Species: Mouse
    Sample Types: Tissue Homogenates
  35. Inhalation tolerance is induced selectively in thoracic lymph nodes but executed pervasively at distant mucosal and nonmucosal tissues.
    Authors: Alvarez D, Swirski FK, Yang TC, Fattouh R, Croitoru K, Bramson JL, Stampfli MR, Jordana M
    J. Immunol., 2006;176(4):2568-80.
    Species: Mouse
    Sample Types: Serum
  36. Immunosuppressant triptolide inhibits dendritic cell-mediated chemoattraction of neutrophils and T cells through inhibiting Stat3 phosphorylation and NF-kappaB activation.
    Authors: Liu Q, Chen T, Chen G, Li N, Wang J, Ma P, Cao X
    Biochem. Biophys. Res. Commun., 2006;345(3):1122-30.
    Species: Mouse
    Sample Types: Cell Culture Supernates

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Mouse CCL17/TARC Quantikine ELISA Kit
By Anonymous on 02/25/2020
Application: Sample Tested: Bone marrow-derived dendritic cells

CCL17 detected following cytokine stimulation


Mouse CCL17/TARC Quantikine ELISA Kit
By Anonymous on 08/16/2017
Application: Sample Tested: Spinal cord

CCL17 protein levels were determined in the spinal cord of naive, pre-clinical, onset, and peak disease mice with EAE.