Mouse CCL17/TARC Quantikine ELISA Kit

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(1 Review)
  
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL)
  • Sensitivity
    5 pg/mL
  • Assay Range
    7.8 - 500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma)
  • Specificity
    Natural and recombinant mouse TARC
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse TARC Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse TARC levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse TARC and antibodies raised against the recombinant factor. Results obtained for naturally occurring mouse TARC showed linear curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse TARC.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020202020
Mean27892782998310
Standard Deviation1.32.37.82.26.917.3
CV%4.82.62.87.675.6

Recovery

The recovery of mouse TARC spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=6) 94 85-113
EDTA Plasma (n=6) 94 83-109
Serum (n=6) 94 82-103
Linearity
To assess the linearity of the assay, five or more samples containing and/or spiked with various concentrations of mouse TARC in each matrix were diluted with Calibrator Diluent and assayed.
Mouse CCL17/TARC Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CCL17/TARC
CCL17/TARC is a chemokine that interacts with CCR4 and CCR8 to induce the chemoattraction of activated Th2 cells, basophils, and NK cells. It is produced by thymic dendritic cells, lymph node dendritic cells, monocytes, CD4+ T cells, keratinocytes, fibroblasts, bronchial epithelial cells, and Reed-Sternberg cells. CCL17 promotes Th2 cell recruitment to sites of inflammation and induces platelet aggregation and degranulation.
    • Entrez Gene IDs
      6361 (Human); 20295 (Mouse);
    • Alternate Names
      ABCD-2; CC chemokine TARC; C-C motif chemokine 17; CCL17; chemokine (C-C motif) ligand 17; MGC138273; SCYA17MGC138271; small inducible cytokine subfamily A (Cys-Cys), member 17; Small-inducible cytokine A17; T cell-directed CC chemokine; TARC; TARCA-152E5.3; Thymus and activation-regulated chemokine;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 50 µL Assay Diluent
    4.   Add 50 µL of Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
    7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

    8. 100 µL Conjugate
    9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
    10.   Aspirate and wash 5 times.

    11. 100 µL Substrate Solution
    12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

    13. 100 µL Stop Solution
    14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    32 Citations: Showing 1 - 10
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    Species
    Sample Type
    1. Dendritic cells induce Th2-mediated airway inflammatory responses to house dust mite via DNA-dependent protein kinase.
      Authors: Mishra, Amarjit, Brown, Alexandr, Yao, Xianglan, Yang, Shutong, Park, Sung-Jun, Liu, Chengyu, Dagur, Pradeep, McCoy, J Philip, Keeran, Karen J, Nugent, Gayle Z, Jeffries, Kenneth, Qu, Xuan, Yu, Zu-Xi, Levine, Stewart, Chung, Jay H
      Nat Commun, 2015;6(0):6224.
      Species: Mouse
      Sample Type: BALF
    2. Dysbiosis and Staphylococcus aureus Colonization Drives Inflammation in Atopic Dermatitis.
      Authors: Kobayashi T, Glatz M, Horiuchi K, Kawasaki H, Akiyama H, Kaplan D, Kong H, Amagai M, Nagao K
      Immunity, 2015;42(4):756-66.
      Species: Mouse
      Sample Type: Serum
    3. Cleavage of the T cell protein tyrosine phosphatase by the hepatitis C virus nonstructural 3/4A protease induces a Th1 to Th2 shift reversible by ribavirin therapy.
      Authors: Brenndorfer E, Brass A, Karthe J, Ahlen G, Bode J, Sallberg M
      J Immunol, 2014;192(4):1671-80.
      Species: Mouse
      Sample Type: Cell Lysates
    4. Hypomorphic mutation in the RAG2 gene affects dendritic cell distribution and migration.
      Authors: Maina, Virginia, Marrella, Veronica, Mantero, Stefano, Cassani, Barbara, Fontana, Elena, Anselmo, Achille, Del Prete, Annalisa, Sozzani, Silvano, Vezzoni, Paolo, Poliani, Pietro L, Villa, Anna
      J Leukoc Biol, 2013;94(6):1221-30.
      Species: Mouse
      Sample Type: Tissue Homogenates
    5. Dual functions of prostaglandin D2 in murine contact hypersensitivity via DP and CRTH2.
      Authors: Yamamoto Y, Otani S, Hirai H, Nagata K, Aritake K, Urade Y, Narumiya S, Yokozeki H, Nakamura M, Satoh T
      Am. J. Pathol., 2012;179(1):302-14.
      Species: Mouse
      Sample Type: Tissue Homogenates
    6. Chitin elicits CCL2 from airway epithelial cells and induces CCR2-dependent innate allergic inflammation in the lung.
      J. Immunol., 2012;189(5):2545-52.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    7. IL-33-activated dendritic cells are critical for allergic airway inflammation.
      Authors: Besnard AG, Togbe D, Guillou N, Erard F, Quesniaux V, Ryffel B
      Eur. J. Immunol., 2011;41(6):1675-86.
      Species: Mouse
      Sample Type: BALF
    8. Thymic stromal lymphopoietin is produced by dendritic cells.
      Authors: Kashyap M, Rochman Y, Spolski R
      J. Immunol., 2011;187(3):1207-11.
      Species: Mouse
      Sample Type: BALF
    9. Endogenous IL-33 enhances Th2 cytokine production and T-cell responses during allergic airway inflammation.
      Authors: Louten J, Rankin AL, Li Y
      Int. Immunol., 2011;23(5):307-15.
      Species: Mouse
      Sample Type: BALF
    10. Reciprocal expression of IL-25 and IL-17A is important for allergic airways hyperreactivity.
      Authors: Barlow JL, Flynn RJ, Ballantyne SJ, McKenzie AN
      Clin. Exp. Allergy, 2011;41(10):1447-55.
      Species: Mouse
      Sample Type: BALF
    11. IL-22 Is Produced by Innate Lymphoid Cells and Limits Inflammation in Allergic Airway Disease.
      Authors: Taube C, Tertilt C, Gyulveszi G, Dehzad N, Kreymborg K, Schneeweiss K, Michel E, Reuter S, Renauld JC, Arnold-Schild D, Schild H, Buhl R, Becher B
      PLoS ONE, 2011;6(7):e21799.
      Species: Mouse
      Sample Type: BALF
    12. IL-33 and M2a alveolar macrophages promote lung defense against the atypical fungal pathogen Pneumocystis murina.
      Authors: Nelson MP, Christmann BS, Werner JL, Metz AE, Trevor JL, Lowell CA, Steele C
      J. Immunol., 2011;186(4):2372-81.
      Species: Mouse
      Sample Type: Tissue Homogenates
    13. Thymic stromal lymphopoietin is a key mediator of breast cancer progression.
      Authors: Olkhanud PB, Rochman Y, Bodogai M
      J. Immunol., 2011;186(10):5656-62.
      Species: Mouse
      Sample Type: Plasma
    14. Foxa2 programs Th2 cell-mediated innate immunity in the developing lung.
      Authors: Chen G, Wan H, Luo F
      J. Immunol., 2010;184(11):6133-41.
      Species: Mouse
      Sample Type: BALF
    15. Murine atopic dermatitis responds to peroxisome proliferator-activated receptors alpha and beta/delta (but not gamma) and liver X receptor activators.
      Authors: Hatano Y, Man MQ, Uchida Y, Crumrine D, Mauro TM, Feingold KR, Elias PM, Holleran WM
      J. Allergy Clin. Immunol., 2010;125(1):160-9.e1-5.
      Species: Mouse
      Sample Type: Serum
    16. CD11b+ myeloid cells are the key mediators of Th2 cell homing into the airway in allergic inflammation.
      Authors: Medoff BD, Seung E, Hong S, Thomas SY, Sandall BP, Duffield JS, Kuperman DA, Erle DJ, Luster AD
      J. Immunol., 2009;182(1):623-35.
      Species: Mouse
      Sample Type: BALF
    17. Maintenance of an acidic stratum corneum prevents emergence of murine atopic dermatitis.
      Authors: Hatano Y, Man MQ, Uchida Y, Crumrine D, Scharschmidt TC, Kim EG, Mauro TM, Feingold KR, Elias PM, Holleran WM
      J. Invest. Dermatol., 2009;129(7):1824-35.
      Species: Mouse
      Sample Type: Serum
    18. Disruption of interleukin-1 signaling improves the quality of wound healing.
      Authors: Thomay AA, Daley JM, Sabo E, Worth PJ, Shelton LJ, Harty MW, Reichner JS, Albina JE
      Am. J. Pathol., 2009;174(6):2129-36.
      Species: Mouse
      Sample Type: Tissue Secretion
    19. Lovastatin inhibits antigen-induced airway eosinophilia without affecting the production of inflammatory mediators in mice.
      Authors: Chiba Y, Sato S, Misawa M
      Inflamm. Res., 2009;0(0):.
      Species: Mouse
      Sample Type: BALF
    20. Allergic pulmonary inflammation in mice is dependent on eosinophil-induced recruitment of effector T cells.
      Authors: Jacobsen EA, Ochkur SI, Pero RS, Taranova AG, Protheroe CA, Colbert DC, Lee NA, Lee JJ
      J. Exp. Med., 2008;205(3):699-710.
      Species: Mouse
      Sample Type: BALF
    21. Cyclooxygenase inhibition during allergic sensitization increases STAT6-independent primary and memory Th2 responses.
      Authors: Zhou W, Newcomb DC, Moore ML, Goleniewska K, O'Neal JF, Peebles RS
      J. Immunol., 2008;181(8):5360-7.
      Species: Mouse
      Sample Type: Tissue Homogenates
    22. An eosinophil immune response characterizes the inflammatory skin disease observed in Tie-2 transgenic mice.
      Authors: Voskas D, Babichev Y, Ling LS, Alami J, Shaked Y, Kerbel RS, Ciruna B, Dumont DJ
      J. Leukoc. Biol., 2008;84(1):59-67.
      Species: Mouse
      Sample Type: Serum
    23. A novel mechanism for CCR4 in the regulation of macrophage activation in bleomycin-induced pulmonary fibrosis.
      Authors: Trujillo G, O'Connor EC, Kunkel SL, Hogaboam CM
      Am. J. Pathol., 2008;172(5):1209-21.
      Species: Mouse
      Sample Type: Tissue Homogenates
    24. Therapeutic targeting of CC ligand 21 or CC chemokine receptor 7 abrogates pulmonary fibrosis induced by the adoptive transfer of human pulmonary fibroblasts to immunodeficient mice.
      Authors: Pierce EM, Carpenter K, Jakubzick C, Kunkel SL, Flaherty KR, Martinez FJ, Hogaboam CM
      Am. J. Pathol., 2007;170(4):1152-64.
      Species: Mouse
      Sample Type: Tissue Homogenates
    25. iNKT cells require CCR4 to localize to the airways and to induce airway hyperreactivity.
      Authors: Meyer EH, Wurbel MA, Staton TL, Pichavant M, Kan MJ, Savage PB, DeKruyff RH, Butcher EC, Campbell JJ, Umetsu DT
      J. Immunol., 2007;179(7):4661-71.
      Species: Mouse
      Sample Type: Tissue Homogenates
    26. Airway epithelial STAT3 is required for allergic inflammation in a murine model of asthma.
      Authors: Simeone-Penney MC, Severgnini M, Tu P, Homer RJ, Mariani TJ, Cohn L, Simon AR
      J. Immunol., 2007;178(10):6191-9.
      Species: Mouse
      Sample Type: BALF
    27. Prolonged inhaled allergen exposure can induce persistent tolerance.
      Authors: Van Hove CL, Maes T, Joos GF, Tournoy KG
      Am. J. Respir. Cell Mol. Biol., 2007;36(5):573-84.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    28. The direct action of 1alpha,25(OH)2-vitamin D3 on purified mouse Langerhans cells.
      Authors: Fujita H, Asahina A, Komine M, Tamaki K
      Cell. Immunol., 2007;245(2):70-9.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    29. Immunosuppressant triptolide inhibits dendritic cell-mediated chemoattraction of neutrophils and T cells through inhibiting Stat3 phosphorylation and NF-kappaB activation.
      Authors: Liu Q, Chen T, Chen G, Li N, Wang J, Ma P, Cao X
      Biochem. Biophys. Res. Commun., 2006;345(3):1122-30.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    30. Inhalation tolerance is induced selectively in thoracic lymph nodes but executed pervasively at distant mucosal and nonmucosal tissues.
      Authors: Alvarez D, Swirski FK, Yang TC, Fattouh R, Croitoru K, Bramson JL, Stampfli MR, Jordana M
      J. Immunol., 2006;176(4):2568-80.
      Species: Mouse
      Sample Type: Serum
    31. Local production of chemokines and prostaglandin E2 in the acute, chronic and recovery phase of murine experimental colitis.
      Authors: Drmotova M, Rehnstrom E, Jansson L
      Cytokine, 2006;35(5):275-83.
      Species: Mouse
      Sample Type: Tissue Homogenates
    32. CCL17 transgenic mice show an enhanced Th2-type response to both allergic and non-allergic stimuli.
      Authors: Tsunemi Y, Saeki H, Nakamura K, Nagakubo D, Nakayama T, Yoshie O, Kagami S, Shimazu K, Kadono T, Sugaya M, Komine M, Matsushima K, Tamaki K
      Eur. J. Immunol., 2006;36(8):2116-27.
      Species: Mouse
      Sample Type: Serum
    Expand to show all 32 Citations
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    : Mouse CCL17/TARC Quantikine ELISA Kit [MCC170]

    Summary

    Sample TestedSpinal cord

    Other Experimental Details

    Other Experimental DetailsCCL17 protein levels were determined in the spinal cord of naive, pre-clinical, onset, and peak disease mice with EAE.

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