Mouse CCL20/MIP-3 alpha Quantikine ELISA Kit

(11 citations)   
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (50 uL), Heparin Plasma (50 uL)
  • Sensitivity
    0.49 pg/mL
  • Assay Range
    3.9 - 250 pg/mL (Cell Culture Supernates, Serum, Heparin Plasma)
  • Specificity
    Natural and recombinant mouse MIP-3 alpha
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse MIP-3 alpha Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse MIP-3 alpha in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse MIP-3 alpha and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse MIP-3 alpha showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse MIP-3 alpha.

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Standard Deviation0.61.75.913.67.5


The recovery of mouse MIP-3 alpha spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=4) 100 96-106
Heparin Plasma (n=4) 98 85-115
Serum (n=4) 106 95-119
To assess the linearity of the assay, samples spiked with recombinant mouse MIP-3 alpha in each matrix were diluted with Calibrator Diluent and then assayed.
Mouse CCL20/MIP-3 alpha Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CCL20/MIP-3 alpha
Macrophage inflammatory protein 3 alpha (MIP-3 alpha), also known as LARC (liver and activation-regulated chemokine) and as exodus-1, is one of many novel CC chemokines identified through bioinformatics. MIP-3 alpha is distantly related to other CC chemokines (20 - 28% amino acid sequence identity). Rat MIP-3 alpha shares approximately 70% and 61% amino acid sequence identity with mouse and human MIP-3 alpha, respectively.
    • Entrez Gene IDs
      6364 (Human); 20297 (Mouse); 29538 (Rat);
    • Alternate Names
      beta chemokine exodus-1; Beta-chemokine exodus-1; CC chemokine LARC; C-C motif chemokine 20; chemokine (C-C motif) ligand 20; CKb4; exodus-1; LARC; LARCLiver and activation-regulated chemokine; MIP3 alpha; MIP-3 alpha; MIP-3a; MIP-3-alpha; MIP3AMacrophage inflammatory protein 3 alpha; SCYA20Small-inducible cytokine A20; small inducible cytokine subfamily A (Cys-Cys), member 20; ST38;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 50 µL Assay Diluent
    4.   Add 50 µL of Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
    7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

    8. 100 µL Conjugate
    9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
    10.   Aspirate and wash 5 times.

    11. 100 µL Substrate Solution
    12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

    13. 100 µL Stop Solution
    14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    Showing Results 1 - 10 of 11
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    Sample Type
    1. Prion infection of mouse brain reveals multiple new upregulated genes involved in neuroinflammation or signal transduction.
      Authors: Carroll J, Striebel J, Race B, Phillips K, Chesebro B
      J Virol, 2015;89(4):2388-404.
      Species: Mouse
      Sample Type: Tissue Homogenates
    2. Potential roles of CCR5(+) CCR6(+) dendritic cells induced by nasal ovalbumin plus Flt3 ligand expressing adenovirus for mucosal IgA responses.
      Authors: Fukuyama Y, Tokuhara D, Sekine S, Aso K, Kataoka K, Davydova J, Yamamoto M, Gilbert R, Tokuhara Y, Fujihashi K, Kunisawa J, Yuki Y, Kiyono H, McGhee J, Fujihashi K
      PLoS ONE, 2013;8(4):e60453.
      Species: Mouse
      Sample Type: cell culture supernate
    3. Monocyte-derived dendritic cell recruitment and allergic T(H)2 responses after exposure to diesel particles are CCR2 dependent.
      Authors: Provoost S, Maes T, Joos GF, Tournoy KG
      J. Allergy Clin. Immunol., 2012;129(2):483-91.
      Species: Mouse
      Sample Type: BALF
    4. CCL20, gammadelta T cells, and IL-22 in corneal epithelial healing.
      Authors: Li Z, Burns AR, Miller SB, Smith CW
      FASEB J., 2011;25(8):2659-68.
      Species: Mouse
      Sample Type: Tissue Homogenates
    5. The Role of ChemR23 in the Induction and Resolution of Cigarette Smoke-Induced Inflammation.
      Authors: Demoor T, Bracke KR, Dupont LL
      J. Immunol., 2011;186(9):5457-67.
      Species: Mouse
      Sample Type: BALF
    6. NOD1 contributes to mouse host defense against Helicobacter pylori via induction of type I IFN and activation of the ISGF3 signaling pathway.
      Authors: Watanabe T, Asano N, Fichtner-Feigl S, Gorelick PL, Tsuji Y, Matsumoto Y, Chiba T, Fuss IJ, Kitani A, Strober W
      J. Clin. Invest., 2010;120(5):1645-62.
      Species: Mouse
      Sample Type: Tissue Homogenates
    7. Th17 Cytokines Stimulate CCL20 Expression in Keratinocytes In Vitro and In Vivo: Implications for Psoriasis Pathogenesis.
      Authors: Harper EG, Guo C, Rizzo H, Lillis JV, Kurtz SE, Skorcheva I, Purdy D, Fitch E, Iordanov M, Blauvelt A
      J. Invest. Dermatol., 2009;129(0):2175.
      Species: Mouse
      Sample Type: Tissue Homogenates
    8. Tyrosine phosphatase SHP-1 in oxidative stress and development of allergic airway inflammation.
      Authors: Cho YS, Oh SY, Zhu Z
      Am. J. Respir. Cell Mol. Biol., 2008;39(4):412-9.
      Species: Mouse
      Sample Type: BALF
    9. Mouse models of rhinovirus-induced disease and exacerbation of allergic airway inflammation.
      Authors: Bartlett NW, Walton RP, Edwards MR, Aniscenko J, Caramori G, Zhu J, Glanville N, Choy KJ, Jourdan P, Burnet J, Tuthill TJ, Pedrick MS, Hurle MJ, Plumpton C, Sharp NA, Bussell JN, Swallow DM, Schwarze J, Guy B, Almond JW, Jeffery PK, Lloyd CM, Papi A, Killington RA, Rowlands DJ, Blair ED, Clarke NJ, Johnston SL
      Nat. Med., 2008;14(2):199-204.
      Species: Mouse
      Sample Type: BALF
    10. Chemokine receptor CCR2 but not CCR5 or CCR6 mediates the increase in pulmonary dendritic cells during allergic airway inflammation.
      Authors: Robays LJ, Maes T, Lebecque S, Lira SA, Kuziel WA, Brusselle GG, Joos GF, Vermaelen KV
      J. Immunol., 2007;178(8):5305-11.
      Species: Mouse
      Sample Type: BALF
    11. An inflammation-induced mechanism for leukocyte transmigration across lymphatic vessel endothelium.
      Authors: Johnson LA, Clasper S, Holt AP, Lalor PF, Baban D, Jackson DG
      J. Exp. Med., 2006;203(12):2763-77.
      Species: Mouse
      Sample Type: Cell Culture Supernates
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