Mouse CCL20/MIP-3 alpha Antibody

Name: Mouse CCL20/MIP-3 alpha Antibody
Brand: R&D Systems
SKU: MAB760-500
Rating: 5 (1 reviews)

MAB760 5 Citations 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB760-SP
MAB760-100
MAB760-500

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Detection of Mouse CCL20 by Western Blot.

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Citations (5)

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Reviews (1)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CCL20/MIP-3 alpha Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CCL20/MIP‑3 alpha in direct ELISAs and Western blots. In direct ELISAs, 12% cross-reactivity with recombinant rat (rr) CCL20, 6% cross-reactivity with recombinant mouse (rm) CCL12 and recombinant human (rh) CCL25, and 3% cross‑reactivity with with rmCCL9/10/MIP-1 gamma is observed. No cross-reactivity with recombinant cotton rat CCL3, 4, 5, rhCCL1, 2, 3, 4, 5, 7, 8, 11,13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 26, 27, 28, rmCCL1, 2, 3, 4, 5, 6, 7, 8, 11, 17, 19, 21, 22, 24, 25, 27, or 28 is observed.

Source

Monoclonal Rat IgG₁ Clone # 114906

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse CCL20/MIP-3 alpha
Ala28-Met97
Accession # O89093

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot

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Detection of Mouse CCL20 by Western Blot. Western blot shows lysates of mouse small intestine. PVDF membrane was probed with 2 µg/mL of Mouse CCL20/MIP-3a Monoclonal Antibody (Catalog # MAB760) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for CCL20 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL20/MIP-3 alpha

CCL20, also known as MIP-3 alpha, LARC, and Exodus, is a beta chemokine. Mouse CCL20 cDNA encodes a 97 amino acid precursor protein with a 27 aa putative signal peptide that is predicted to be cleaved to form the 70 aa mature secreted protein. CCL20 is distantly related to other beta chemokines (20‑28% aa sequence identity). Mouse CCL20 shares approximately 71 and 63% amino acid sequence homology with rat and human CCL20, respectively. CCL20 has been shown to be expressed predominantly in lymph nodes, appendix, PBL, fetal liver, fetal lung, and epithelial cells of intestinal tissues. The expression of CCL20 is strongly upregulated by inflammatory signals and downregulated by the anti-inflammatory cytokine IL-10. CCL20 has been shown to be chemotactic for lymphocytes and dendritic cells, and inhibits proliferation of myeloid progenitors in colony formation assays. CCL20 is a functional ligand for CCR6 (previously referred to as GPR-CY4, CKR-L3, or STRL22 orphan receptor), a chemokine receptor that is selectively and highly expressed in human dendritic cells derived from CD34⁺ cord blood precursors.

References

Baba, M. et al. (1997) J. Biol. Chem. 272:14893.
Hromas, R. et al. (1997) Blood 89:3315.
Greaves, D. R. et al. (1997) J. Exp. Med. 186: 857.
Tanaka, Y. et al. (1999) Eur. J. Immunol. 29:633.

Entrez Gene IDs

6364 (Human); 20297 (Mouse); 29538 (Rat)

Alternate Names

beta chemokine exodus-1; Beta-chemokine exodus-1; CC chemokine LARC; C-C motif chemokine 20; CCL20; chemokine (C-C motif) ligand 20; CKb4; exodus-1; LARC; LARCLiver and activation-regulated chemokine; MIP3 alpha; MIP-3 alpha; MIP-3a; MIP-3-alpha; MIP3AMacrophage inflammatory protein 3 alpha; SCYA20Small-inducible cytokine A20; small inducible cytokine subfamily A (Cys-Cys), member 20; ST38

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse CCL20/MIP-3 alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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V&gamma4 T cell-derived IL-17A is essential for amplification of inflammatory cascades in ischemic brain tissue after stroke
Authors: L Lu, Y Wang, L Zhou, Y Li, X Zhang, X Hu, S Shi, W He
International immunopharmacology, 2021;96(0):107678.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization
Role of S1P/S1PR3 axis in release of CCL20 from human bronchial epithelial cells
Authors: Y Kawa, T Nagano, A Yoshizaki, R Dokuni, M Katsurada, T Terashita, Y Yasuda, K Umezawa, M Yamamoto, H Kamiryo, K Kobayashi, Y Nishimura
PLoS ONE, 2018;13(9):e0203211.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization
Leukocyte attraction by CCL20 and its receptor CCR6 in humans and mice with pneumococcal meningitis.
Authors: Klein M, Brouwer M, Angele B, Geldhoff M, Marquez G, Varona R, Hacker G, Schmetzer H, Hacker H, Hammerschmidt S, van der Ende A, Pfister H, van de Beek D, Koedel U
PLoS ONE, 2014;9(4):e93057.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization
Near-infrared laser adjuvant for influenza vaccine.
Authors: Kashiwagi, Satoshi, Yuan, Jianping, Forbes, Benjamin, Hibert, Mathew L, Lee, Eugene L, Whicher, Laura, Goudie, Calum, Yang, Yuan, Chen, Tao, Edelblute, Beth, Collette, Brian, Edington, Laurel, Trussler, James, Nezivar, Jean, Leblanc, Pierre, Bronson, Roderick, Tsukada, Kosuke, Suematsu, Makoto, Dover, Jeffrey, Brauns, Timothy, Gelfand, Jeffrey, Poznansky, Mark C
PLoS ONE, 2013;8(12):e82899.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Contribution of IL-17-producing gamma delta T cells to the efficacy of anticancer chemotherapy.
Authors: Ma Y, Aymeric L, Locher C, Mattarollo SR, Delahaye NF, Pereira P, Boucontet L, Apetoh L, Ghiringhelli F, Casares N, Lasarte JJ, Matsuzaki G, Ikuta K, Ryffel B, Benlagha K, Tesniere A, Ibrahim N, Dechanet-Merville J, Chaput N, Smyth MJ, Kroemer G, Zitvogel L
J. Exp. Med., 2011;208(3):491-503.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization