Mouse CCL22/MDC Quantikine ELISA Kit

(13 citations)
(1 Review)
  
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (17 uL), EDTA Plasma (17 uL), Heparin Plasma (17 uL)
  • Sensitivity
    1.8 pg/mL
  • Assay Range
    7.8 - 500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Natural and recombinant mouse MDC
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse MDC Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse MDC in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse MDC and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse MDC showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse MDC.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020242424
Mean19.951.83822052.6383
Standard Deviation1.32.519.21.72.928.7
CV%6.54.858.55.57.5

Recovery

The recovery of mouse MDC spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=7) 102 87-112
EDTA Plasma (n=4) 94 89-98
Heparin Plasma (n=4) 90 80-109
Serum (n=5) 91 82-100
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse MDC in each matrix were diluted with Calibrator Diluent and then assayed.
Mouse CCL22/MDC Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CCL22/MDC
Macrophage-derived chemokine (MDC), also named stimulated T cell chemotactic protein (STCP-1) and ABCD-1, and now designated as CCL22, is a CC chemokine initially isolated from clones of monocyte-derived macrophages. At the amino acid sequence level, MDC shows less than 35% identity to other CC chemokine family members. Human MDC is expressed in dendritic cells, macrophages and activated monocytes. In addition, MDC expression is detected in thymus, lymph node and appendix tissues. At the amino acid sequence level, mouse and human MDC share 64% identity and 83% similarity.
    • Entrez Gene IDs
      6367 (Human); 20299 (Mouse);
    • Alternate Names
      A-152E5.1; ABCD-1; CC chemokine STCP-1; C-C motif chemokine 22; CCL22; chemokine (C-C motif) ligand 22; DC/B-CK; Macrophage-derived chemokine; MDC; MDCStimulated T-cell chemotactic protein 1; MGC34554; SCYA22MDC(1-69); small inducible cytokine A22; small inducible cytokine subfamily A (Cys-Cys), member 22; Small-inducible cytokine A22; STCP-1; stimulated T cell chemotactic protein 1;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 50 µL Assay Diluent
    4.   Add 50 µL of Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
    7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

    8. 100 µL Conjugate
    9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
    10.   Aspirate and wash 5 times.

    11. 100 µL Substrate Solution
    12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

    13. 100 µL Stop Solution
    14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    13 Citations: Showing 1 - 10
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    Species
    Sample Type
    1. CCL11 promotes migration and proliferation of mouse neural progenitor cells
      Authors: F Wang, N Baba, Y Shen, T Yamashita, E Tsuru, M Tsuda, N Maeda, Y Sagara
      Stem Cell Res Ther, 2017;8(1):26.
      Species: Mouse
      Sample Type: Tissue Homogenates
    2. Dendritic cells induce Th2-mediated airway inflammatory responses to house dust mite via DNA-dependent protein kinase.
      Authors: Mishra, Amarjit, Brown, Alexandr, Yao, Xianglan, Yang, Shutong, Park, Sung-Jun, Liu, Chengyu, Dagur, Pradeep, McCoy, J Philip, Keeran, Karen J, Nugent, Gayle Z, Jeffries, Kenneth, Qu, Xuan, Yu, Zu-Xi, Levine, Stewart, Chung, Jay H
      Nat Commun, 2015;6(0):6224.
      Species: Mouse
      Sample Type: BALF
    3. Cleavage of the T cell protein tyrosine phosphatase by the hepatitis C virus nonstructural 3/4A protease induces a Th1 to Th2 shift reversible by ribavirin therapy.
      Authors: Brenndorfer E, Brass A, Karthe J, Ahlen G, Bode J, Sallberg M
      J Immunol, 2014;192(4):1671-80.
      Species: Mouse
      Sample Type: Cell Lysates
    4. Dual functions of prostaglandin D2 in murine contact hypersensitivity via DP and CRTH2.
      Authors: Yamamoto Y, Otani S, Hirai H, Nagata K, Aritake K, Urade Y, Narumiya S, Yokozeki H, Nakamura M, Satoh T
      Am. J. Pathol., 2012;179(1):302-14.
      Species: Mouse
      Sample Type: Tissue Homogenates
    5. Chitin elicits CCL2 from airway epithelial cells and induces CCR2-dependent innate allergic inflammation in the lung.
      J. Immunol., 2012;189(5):2545-52.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    6. Prevention of murine autoimmune diabetes by CCL22-mediated Treg recruitment to the pancreatic islets.
      Authors: Montane J, Bischoff L, Soukhatcheva G, Dai DL, Hardenberg G, Levings MK, Orban PC, Kieffer TJ, Tan R, Verchere CB
      J. Clin. Invest., 2011;121(0):3024.
      Species: Mouse
      Sample Type: Cell Lysates
    7. IL-33 and M2a alveolar macrophages promote lung defense against the atypical fungal pathogen Pneumocystis murina.
      Authors: Nelson MP, Christmann BS, Werner JL, Metz AE, Trevor JL, Lowell CA, Steele C
      J. Immunol., 2011;186(4):2372-81.
      Species: Mouse
      Sample Type: Tissue Homogenates
    8. CD11b+ myeloid cells are the key mediators of Th2 cell homing into the airway in allergic inflammation.
      Authors: Medoff BD, Seung E, Hong S, Thomas SY, Sandall BP, Duffield JS, Kuperman DA, Erle DJ, Luster AD
      J. Immunol., 2009;182(1):623-35.
      Species: Mouse
      Sample Type: BALF
    9. Role of the chemokine decoy receptor D6 in balancing inflammation, immune activation, and antimicrobial resistance in Mycobacterium tuberculosis infection.
      Authors: Di Liberto D, Locati M, Caccamo N, Vecchi A, Meraviglia S, Salerno A, Sireci G, Nebuloni M, Caceres N, Cardona PJ, Dieli F, Mantovani A
      J. Exp. Med., 2008;205(9):2075-84.
      Species: Mouse
      Sample Type: Serum
    10. Allergic pulmonary inflammation in mice is dependent on eosinophil-induced recruitment of effector T cells.
      Authors: Jacobsen EA, Ochkur SI, Pero RS, Taranova AG, Protheroe CA, Colbert DC, Lee NA, Lee JJ
      J. Exp. Med., 2008;205(3):699-710.
      Species: Mouse
      Sample Type: BALF
    11. A novel mechanism for CCR4 in the regulation of macrophage activation in bleomycin-induced pulmonary fibrosis.
      Authors: Trujillo G, O'Connor EC, Kunkel SL, Hogaboam CM
      Am. J. Pathol., 2008;172(5):1209-21.
      Species: Mouse
      Sample Type: Tissue Homogenates
    12. Protection against inflammation- and autoantibody-caused fetal loss by the chemokine decoy receptor D6.
      Authors: Martinez de la Torre Y, Buracchi C, Borroni EM, Dupor J, Bonecchi R, Nebuloni M, Pasqualini F, Doni A, Lauri E, Agostinis C, Bulla R, Cook DN, Haribabu B, Meroni P, Rukavina D, Vago L, Tedesco F, Vecchi A, Lira SA, Locati M, Mantovani A
      Proc. Natl. Acad. Sci. U.S.A., 2007;104(7):2319-24.
      Species: Mouse
      Sample Type: Serum
    13. iNKT cells require CCR4 to localize to the airways and to induce airway hyperreactivity.
      Authors: Meyer EH, Wurbel MA, Staton TL, Pichavant M, Kan MJ, Savage PB, DeKruyff RH, Butcher EC, Campbell JJ, Umetsu DT
      J. Immunol., 2007;179(7):4661-71.
      Species: Mouse
      Sample Type: Tissue Homogenates
    Expand to show all 13 Citations
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    : Mouse CCL22/MDC Quantikine ELISA Kit [MCC220]

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