Mouse CCL5/RANTES Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF478
Product Details
Citations (7)
FAQs
Supplemental Products
Reviews (1)

Mouse CCL5/RANTES Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CCL5/RANTES in ELISAs and Western blots. In sandwich immunoassays, approximately 1% cross-reactivity with recombinant human (rh) CCL5 is observed and less than 0.1% cross-reactivity with recombinant mouse VIC, rhVIC, and rhTECK is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse CCL5/RANTES
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse CCL5/RANTES (Catalog # 478-MR)

Mouse CCL5/RANTES Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Mouse CCL5/RANTES Antibody (Catalog # MAB4781)

Standard: Recombinant Mouse CCL5/RANTES Protein (Catalog # 478-MR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL5/RANTES

CCL5, also known as RANTES (Regulated upon Activation, Normal T cell Expressed and presumably Secreted), is an 8 kDa beta -chemokine that plays a primary role in the inflammatory immune response by means of its ability to attract and activate leukocytes (1 - 3). Human and mouse RANTES exhibit cross-species activity on human and mouse cells (4). Mature mouse CCL5 shares 100% aa sequence identity with rat CCL5 and 75% - 88% with canine, cotton rat, feline, and human CCL5 (5). CCL5 is secreted by many cell types at inflammatory sites, and it exerts a wide range of activities through the receptors CCR1, CCR3, CCR4, and CCR5 (6, 7). Inflammatory responses can be impaired by the sequestration of CCL5 by the cytomegalovirus protein US28 (8). In humans, CCR5 binding to CCL5 inhibits the infectivity of R5 (M-tropic) but not X4 (T-tropic) strains of HIV-1 (9). The two N-terminal residues of CCL5 can be removed by CD26/DPPIV, generating a protein that functions as a chemotaxis inhibitor and more effectively blocks M-tropic HIV-1 infection of monocytes (10). Oligomerization of CCL5 on glycosaminoglycans is required for CCR1-mediated leukocyte adhesion and activation as well as CCL5’s interaction with the chemokine CXCL4/PF4 (11 - 13). The deposition of CCL5 on activated vascular endothelial cells is crucial for monocyte adhesion to damaged vasculature, but CCL5 oligomerization is not required for the extravasation of adherent leukocytes (14 - 16). CCL5 is upregulated in breast cancer and promotes tumor progression through the attraction of proinflammatory macrophages in addition to its actions on tumor cells, stromal cells, and the vasculature (17).

References
  1. Schall, T.J. et al. (1990) Nature 347:669.
  2. Bacon, K.B. et al. (1995) Science 269:1727.
  3. Fischer, F.R. et al. (2001) J. Immunol. 167:1637.
  4. Schall, T.J. et al. (1992) Eur. J. Immunol. 22:1477.
  5. Heeger, P. et al. (1992) Kidney Int. 41:220.
  6. Appay, V. and S.L. Rowland-Jones (2001) Trends Immunol. 22:83.
  7. Levy, J.A. (2009) J. Immunol. 182:3945.
  8. Randolph-Habecker, J.R. et al. (2002) Cytokine 19:37.
  9. DeVico, A.L. and R.C. Gallo (2004) Nat. Rev. Microbiol. 2:401.
  10. Proost, P. et al. (1998) J. Biol. Chem. 273:7222.
  11. Appay, V. et al. (1999) J. Biol. Chem. 274:27505.
  12. Proudfoot, A.E.I. et al. (2003) Proc. Natl. Acad. Sci. USA 100:1885.
  13. von Hundelshausen, P. et al. (2005) Blood 105:924.
  14. von Hundelshausen, P. et al. (2001) Circulation 103:1772.
  15. Zernecke, A. et al. (2008) Arterioscler. Thromb. Vasc. Biol. 28:1897.
  16. Baltus, T. et al. (2003) Blood 102:1985.
  17. Soria, G. and A. Ben-Baruch (2008) Cancer Lett. 267:271.
Entrez Gene IDs
6352 (Human); 20304 (Mouse); 403522 (Canine); 493689 (Feline)
Alternate Names
CCL5; chemokine (C-C motif) ligand 5; D17S136Enormally T-expressed, and presumably secreted; EoCP; Eosinophil chemotactic cytokine; RANTES; SISd; SIS-delta; small inducible cytokine A5 (RANTES); small inducible cytokine subfamily A (Cys-Cys), member 5; Small-inducible cytokine A5; T cell-specific protein P228; T-cell specific protein p288; TCP228T-cell-specific protein RANTES

Product Datasheets

Citations for Mouse CCL5/RANTES Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Nitrosporeusine analogue ameliorates Chandipura virus induced inflammatory response in CNS via NF?b inactivation in microglia
    Authors: AK Verma, TS Waghmare, GR Jachak, SC Philkhana, DS Reddy, A Basu
    PLoS Negl Trop Dis, 2018;12(7):e0006648.
    Species: Mouse
    Sample Types: Protein
    Applications: ELISA detection
  2. Cancer cell CCL5 mediates bone marrow independent angiogenesis in breast cancer
    Oncotarget, 2016;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: ELISA detection
  3. KIM-1-mediated phagocytosis reduces acute injury to the kidney.
    Authors: Yang L, Brooks C, Xiao S, Sabbisetti V, Yeung M, Hsiao L, Ichimura T, Kuchroo V, Bonventre J
    J Clin Invest, 2015;125(4):1620-36.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: ELISA detection
  4. Radiation-inducible immunotherapy for cancer: senescent tumor cells as a cancer vaccine.
    Authors: Meng Y, Efimova EV, Hamzeh KW
    Mol. Ther., 2012;20(5):1046-55.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  5. Diesel exhaust particulates exacerbate asthma-like inflammation by increasing CXC chemokines.
    Authors: Kim J, Natarajan S, Vaickus LJ, Bouchard JC, Beal D, Cruikshank WW, Remick DG
    Am. J. Pathol., 2011;179(6):2730-9.
    Species: Mouse
    Sample Types: BALF
    Applications: ELISA Development
  6. Synthesis of several chemokines but few cytokines by primed uncommitted precursor CD4 T cells suggests that these cells recruit other immune cells without exerting direct effector functions.
    Authors: Yang L, Mosmann T
    Eur. J. Immunol., 2004;34(6):1617-26.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  7. Synthesis of several chemokines but few cytokines by primed uncommitted precursor CD4 T cells suggests that these cells recruit other immune cells without exerting direct effector functions.
    Authors: Mosmann T
    Eur. J. Immunol., 2004;34(6):1617-26.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development

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Mouse CCL5/RANTES Biotinylated Antibody
By Anonymous on 11/27/2017
Application: ELISA Sample Tested: Serum and Plasma Species: Mouse

This antibody was used as the detection with MAB478 as the capture in an ELISA.