Mouse CCL5/RANTES Antibody

(15 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CCL5/RANTES in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant human (rh) CCL5/RANTES and recombinant cotton rat CCL5/RANTES is observed, and less than 3% cross-reactivity with rhCCL3/MIP-1 alpha and recombinant mouse CCL3/MIP-1 alpha is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant mouse CCL5/RANTES
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.30 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse CCL5/RANTES (Catalog # 478-MR)
  • Immunocytochemistry
    5-15 µg/mL
    See below
  • Neutralization
    Measured by its ability to neutralize CCL5/RANTES-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR5. The Neutralization Dose (ND50) is typically 0.07-0.3 µg/mL in the presence of 0.05 µg/mL Recombinant Mouse CCL5/RANTES.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Chemotaxis Induced by CCL5/RANTES and Neutralization by Mouse CCL5/RANTES Antibody. Recombinant Mouse CCL5/RANTES (Catalog # 478‑MR) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL5/RANTES (0.05 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL5/
RANTES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF478). The ND50 is typically 0.07-0.3 µg/mL.
Immunocytochemistry
CCL5/RANTES in Mouse Splenocytes. CCL5/RANTES was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse CCL5/RANTES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF478) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL5/RANTES
CCL5, also known as RANTES (Regulated upon Activation, Normal T cell Expressed and presumably Secreted), is an 8 kDa beta -chemokine that plays a primary role in the inflammatory immune response by means of its ability to attract and activate leukocytes (1-3). Human and mouse RANTES exhibit cross-species activity on human and mouse cells (4). Mature mouse CCL5 shares 100% aa sequence identity with rat CCL5 and 75-88% with canine, cotton rat, feline, and human CCL5 (5). CCL5 is secreted by many cell types at inflammatory sites, and it exerts a wide range of activities through the receptors CCR1, CCR3, CCR4, and CCR5 (6, 7). Inflammatory responses can be impaired by the sequestration of CCL5 by the cytomegalovirus protein US28 (8). In humans, CCR5 binding to CCL5 inhibits the infectivity of R5 (M-tropic) but not X4 (T-tropic) strains of HIV-1 (9). The two N-terminal residues of CCL5 can be removed by CD26/DPPIV, generating a protein that functions as a chemotaxis inhibitor and more effectively blocks M-tropic HIV-1 infection of monocytes (10). Oligomerization of CCL5 on glycosaminoglycans is required for CCR1-mediated leukocyte adhesion and activation as well as CCL5’s interaction with the chemokine CXCL4/PF4 (11-13). The deposition of CCL5 on activated vascular endothelial cells is crucial for monocyte adhesion to damaged vasculature, but CCL5 oligomerization is not required for the extravasation of adherent leukocytes (14-16). CCL5 is upregulated in breast cancer and promotes tumor progression through the attraction of pro-inflammatory macrophages in addition to its actions on tumor cells, stromal cells, and the vasculature (17).
  • References:
    1. Schall, T.J. et al. (1990) Nature 347:669.
    2. Bacon, K.B. et al. (1995) Science 269:1727.
    3. Fischer, F.R. et al. (2001) J. Immunol. 167:1637.
    4. Schall, T.J. et al. (1992) Eur. J. Immunol. 22:1477.
    5. Heeger, P. et al. (1992) Kidney Int. 41:220.
    6. Appay, V. and S.L. Rowland-Jones (2001) Trends Immunol. 22:83.
    7. Levy, J.A. (2009) J. Immunol. 182:3945.
    8. Randolph-Habecker, J.R. et al. (2002) Cytokine 19:37.
    9. DeVico, A.L. and Gallo, R.C. (2004) Nat. Rev. Microbiol. 2:401.
    10. Proost, P. et al. (1998) J. Biol. Chem. 273:7222.
    11. Appay, V. et al. (1999) J. Biol. Chem. 274:27505.
    12. Proudfoot, A.E.I. et al. (2003) Proc. Natl. Acad. Sci. USA 100:1885.
    13. von Hundelshausen, P. et al. (2005) Blood 105:924.
    14. von Hundelshausen, P. et al. (2001) Circulation 103:1772.
    15. Zernecke, A. et al. (2008) Arterioscler. Thromb. Vasc. Biol. 28:1897.
    16. Baltus, T. et al. (2003) Blood 102:1985.
    17. Soria, G. and A. Ben-Baruch (2008) Cancer Lett. 267:271.
  • Entrez Gene IDs:
    6352 (Human); 20304 (Mouse); 403522 (Canine); 493689 (Feline)
  • Alternate Names:
    CCL5; chemokine (C-C motif) ligand 5; D17S136Enormally T-expressed, and presumably secreted; EoCP; Eosinophil chemotactic cytokine; RANTES; SISd; SIS-delta; small inducible cytokine A5 (RANTES); small inducible cytokine subfamily A (Cys-Cys), member 5; Small-inducible cytokine A5; T cell-specific protein P228; T-cell specific protein p288; TCP228T-cell-specific protein RANTES
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. IFN? Protects Neurons from Damage in a Murine Model of HIV-1 Associated Brain Injury
    Authors: VE Thaney, AM O'Neill, MM Hoefer, R Maung, AB Sanchez, M Kaul
    Sci Rep, 2017;7(0):46514.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  2. Neutralization of regulated on activation, normal T cell expressed and secreted and eotaxin prevents the loss of dopaminergic neurons in a mouse model of Parkinson's disease
    J Biol Chem, 2016;0(0):.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut
  3. Therapeutic antiviral T cells noncytopathically clear persistently infected microglia after conversion into antigen-presenting cells.
    Authors: Herz J, Johnson K, McGavern D
    J Exp Med, 2015;212(8):1153-69.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  4. Vitamin D limits chemokine expression in adipocytes and macrophage migration in vitro and in male mice.
    Authors: Karkeni E, Marcotorchino J, Tourniaire F, Astier J, Peiretti F, Darmon P, Landrier J
    Endocrinology, 2015;156(5):1782-93.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  5. Astrocytic TGF-beta signaling limits inflammation and reduces neuronal damage during central nervous system Toxoplasma infection.
    Authors: Cekanaviciute E, Dietrich H, Axtell R, Williams A, Egusquiza R, Wai K, Koshy A, Buckwalter M
    J Immunol, 2014;193(1):139-49.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  6. Peptide inhibitor of CXCL4-CCL5 heterodimer formation, MKEY, inhibits experimental aortic aneurysm initiation and progression.
    Authors: Iida, Yasunori, Xu, Baohui, Xuan, Haojun, Glover, Keith J, Tanaka, Hiroki, Hu, Xiaolei, Fujimura, Naoki, Wang, Wei, Schultz, Joshua R, Turner, Court R, Dalman, Ronald L
    Arterioscler Thromb Vasc Biol, 2013;33(4):718-26.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  7. Changes in gene expression of pial vessels of the blood brain barrier during murine neurocysticercosis.
    Authors: Mishra, Pramod K, Teale, Judy M
    PLoS Negl Trop Dis, 2013;7(3):e2099.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC OCT-embedded
  8. Comprehensive assessment of chemokine expression profiles by flow cytometry.
    Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
    J. Clin. Invest., 2010;120(3):907-23.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  9. Selectin-mediated activation of endothelial cells induces expression of CCL5 and promotes metastasis through recruitment of monocytes.
    Authors: Laubli H, Spanaus KS, Borsig L
    Blood, 2009;114(20):4583-91.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  10. CCL3-CCR5 axis regulates intratumoral accumulation of leukocytes and fibroblasts and promotes angiogenesis in murine lung metastasis process.
    Authors: Wu Y, Li YY, Matsushima K, Baba T, Mukaida N
    J. Immunol., 2008;181(9):6384-93.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  11. Expanded-polyglutamine huntingtin protein suppresses the secretion and production of a chemokine (CCL5/RANTES) by astrocytes.
    Authors: Chou SY, Weng JY, Lai HL, Liao F, Sun SH, Tu PH, Dickson DW, Chern Y
    J. Neurosci., 2008;28(13):3277-90.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  12. Viral infection of the lungs through the eye.
    Authors: Bitko V, Musiyenko A, Barik S
    J. Virol., 2007;81(2):783-90.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  13. TWEAK/Fn14 interactions are instrumental in the pathogenesis of nephritis in the chronic graft-versus-host model of systemic lupus erythematosus.
    Authors: Zhao Z, Burkly LC, Campbell S, Schwartz N, Molano A, Choudhury A, Eisenberg RA, Michaelson JS, Putterman C
    J. Immunol., 2007;179(11):7949-58.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  14. A distinct and unique transcriptional program expressed by tumor-associated macrophages (defective NF-kappaB and enhanced IRF-3/STAT1 activation).
    Authors: Biswas SK, Gangi L, Paul S, Schioppa T, Saccani A, Sironi M, Bottazzi B, Doni A, Vincenzo B, Pasqualini F, Vago L, Nebuloni M, Mantovani A, Sica A
    Blood, 2005;107(5):2112-22.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  15. CC chemokines mediate leukocyte trafficking into the central nervous system during murine neurocysticercosis: role of gamma delta T cells in amplification of the host immune response.
    Authors: Cardona AE, Gonzalez PA, Teale JM
    Infect. Immun., 2003;71(5):2634-42.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
Expand to show all 15 Citations
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