Mouse CCL6/C10 Antibody (262016)

R&D Systems | Catalog # MAB487

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Neutralization, Intracellular Staining by Flow Cytometry, CyTOF-ready

Cited:

Neutralization, Flow Cytometry, ELISA Development, In vivo assay

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 262016
View all CCL6/C10 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse CCL6/C10
Gly22-Ala116
Accession # P27784

Specificity

Detects mouse CCL6/C10 in Western blots. In Western blots, no cross-reactivity with recombinant human CCL1, 2, 3, 4, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 19, 21, 22, 23, 24, 25, 28, recombinant mouse CCL3, 4, 9, 11, 12, 19, 20, 21, 22, 24, 25, 28, or recombinant rat CCL20  is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse CCL6/C10 Antibody (262016)

Chemotaxis Induced by CCL6/C10 and Neutral-ization by Mouse CCL6/C10 Antibody.

Chemotaxis Induced by CCL6/C10 and Neutral-ization by Mouse CCL6/C10 Antibody.

Recombinant Mouse CCL6/C10 (Catalog # 487-C) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemo-taxis elicited by Recombinant Mouse CCL6/C10 (1 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCL6/C10 Monoclonal Antibody (Catalog # MAB487). The ND50 is typically 6-24 µg/mL.

Applications for Mouse CCL6/C10 Antibody (262016)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/106 cells
Sample: Mouse bone marrow macrophages fixed with paraformaldehyde and permeabilized with saponin

Western Blot

1 µg/mL
Sample: Recombinant Mouse CCL6/C10 (Catalog # 487-C) under non-reducing conditions only

Neutralization

Measured by its ability to neutralize CCL6/C10-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR1. The Neutralization Dose (ND50) is typically 6-24 µg/mL in the presence of 1 µg/mL Recombinant Mouse CCL6/C10.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL6/C10

CCL6, also known as C10 and SCYA6, is a beta chemokine with an extended N-terminus. CCL6 expression is induced by GM-CSF stimulation of monocytes and neutrophils and IL-4 stimulation of macrophages. CCL6 functions as a monocyte and macrophage chemoattractant.

Alternate Names

C10, MRP-1, SCYA6

Entrez Gene IDs

20305 (Mouse); 287910 (Rat)

Gene Symbol

CCL6

UniProt

P27784

Additional CCL6/C10 Products

Product Documents for Mouse CCL6/C10 Antibody (262016)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse CCL6/C10 Antibody (262016)

For research use only

Citations for Mouse CCL6/C10 Antibody (262016)

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