Detects mouse CCL6/C10 in Western blots. In Western blots, no
cross-reactivity with recombinant human CCL1, 2, 3, 4, 5, 7, 8, 11, 13, 14,
15, 16, 17, 18, 19, 21, 22, 23, 24, 25, 28, recombinant mouse CCL3, 4, 9, 11,
12, 19, 20, 21, 22, 24, 25, 28, or recombinant rat CCL20 is observed.
Monoclonal Rat IgG2B Clone # 262016
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant mouse CCL6/C10 Gly22-Ala116 Accession # P27784
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Recombinant Mouse CCL6/C10 (Catalog # 487-C) under non-reducing conditions only
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
Mouse bone marrow macrophages fixed with paraformaldehyde and permeabilized with saponin
Measured by its ability to neutralize CCL6/C10-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR1. The Neutralization Dose (ND50) is typically 6-24 µg/mL in the presence of 1 µg/mL Recombinant Mouse CCL6/C10.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Chemotaxis Induced by CCL6/C10 and Neutralization by Mouse CCL6/C10 Antibody. Recombinant Mouse CCL6/C10 (Catalog # 487-C) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL6/C10 (1 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCL6/C10 Monoclonal Antibody (Catalog # MAB487). The ND50 is typically 6‑24 µg/mL.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
CCL6, also known as C10 and SCYA6, is a beta chemokine with an extended N-terminus. CCL6 expression is induced by GM-CSF stimulation of monocytes and neutrophils and IL-4 stimulation of macrophages. CCL6 functions as a monocyte and macrophage chemoattractant.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.