Mouse CCL6/C10 Antibody

Catalog # Availability Size / Price Qty
MAB487
MAB487-SP
Mouse CCL6/C10 Antibody in Data
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Product Details
Citations (2)
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Mouse CCL6/C10 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CCL6/C10 in Western blots. In Western blots, no cross-reactivity with recombinant human CCL1, 2, 3, 4, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 19, 21, 22, 23, 24, 25, 28, recombinant mouse CCL3, 4, 9, 11, 12, 19, 20, 21, 22, 24, 25, 28, or recombinant rat CCL20  is observed.
Source
Monoclonal Rat IgG2B Clone # 262016
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse CCL6/C10
Gly22-Ala116
Accession # P27784
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse CCL6/C10 (Catalog # 487-C) under non-reducing conditions only
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
Mouse bone marrow macrophages fixed with paraformaldehyde and permeabilized with saponin
Neutralization
Measured by its ability to neutralize CCL6/C10-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR1. The Neutralization Dose (ND50) is typically 6-24 µg/mL in the presence of 1 µg/mL Recombinant Mouse CCL6/C10.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization Chemotaxis Induced by CCL6/C10 and Neutral-ization by Mouse CCL6/C10 Antibody. View Larger

Chemotaxis Induced by CCL6/C10 and Neutral-ization by Mouse CCL6/C10 Antibody. Recombinant Mouse CCL6/C10 (Catalog # 487-C) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemo-taxis elicited by Recombinant Mouse CCL6/C10 (1 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCL6/C10 Monoclonal Antibody (Catalog # MAB487). The ND50is typically 6-24 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL6/C10

CCL6, also known as C10 and SCYA6, is a beta chemokine with an extended N-terminus. CCL6 expression is induced by GM-CSF stimulation of monocytes and neutrophils and IL-4 stimulation of macrophages. CCL6 functions as a monocyte and macrophage chemoattractant.

Entrez Gene IDs
20305 (Mouse); 287910 (Rat)
Alternate Names
C10; CCL6; MRP-1; SCYA6

Product Datasheets

Citations for Mouse CCL6/C10 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Comprehensive assessment of chemokine expression profiles by flow cytometry.
    Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
    J. Clin. Invest., 2010;120(3):907-23.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. A novel role for constitutively expressed epithelial-derived chemokines as antibacterial peptides in the intestinal mucosa.
    Authors: Kotarsky K, Sitnik KM, Stenstad H, Kotarsky H, Schmidtchen A, Koslowski M, Wehkamp J, Agace WW
    Mucosal Immunol, 2010;3(1):40-8.
    Species: Mouse
    Sample Types: Luminal Lavage
    Applications: ELISA Development

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