Mouse CCR2 Antibody Summary
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of CCR2 in NS0 Mouse Cell Line Transfected with Mouse CCR2 and eGFP by Flow Cytometry. NS0 mouse myeloma cell line transfected with either (A) mouse CCR2 or (B) irrelevant transfectants and eGFP was stained with Rat Anti-Mouse/Rat CCR2 Monoclonal Antibody (Catalog # MAB55381R) followed by APC-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). Quadrant markers were set based on isotype control (Rat IgG2B,Catalog # MAB0061; data not shown). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
CCR2, also known as CD192, is a 38 kDa 7TM chemokine receptor that binds CCL2, CCL7, CCL8, CCL12 (in mouse only) and CCL13. CCR2 is expressed by multiple hematopoietic cells plus, endothelial cells, fibroblasts, and smooth muscle cells. It functions as an HIV fusion co-factor and facilitates T cell recruitment during inflammation. Mouse CCR2 shares 67% and 95% aa sequence identity with human and rat CCR2, respectively.
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