Mouse CD109 Antibody

Catalog # Availability Size / Price Qty
AF7717
AF7717-SP
Detection of Mouse CD109 by Western Blot.
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Mouse CD109 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CD109 in direct ELISAs and Western blots. In direct ELISAs, approximately 65% cross-reactivity with recombinant human CD109 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse CD109
Ala22-Ser1269
Accession # Q8R422
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Mouse CD109 by Western Blot. View Larger

Detection of Mouse CD109 by Western Blot. Western blot shows lysates of C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.1 µg/mL of Sheep Anti-Mouse CD109 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7717) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for CD109 at approximately 170 and 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD109

CD109 (also CPAMD7, p180, r150, Gov antigen and GPI-anchored alpha 2-macroglobulin-related protein) is a 170-180 kDa member of the I39 protease inhibitor/ alpha 2-macroglobulin family of thioester-containing proteins. It is expressed by endothelium, activated platelets and T cells, megakaryocyte lineage stem cells, myoepithalial cells, fibroblasts and keratinocytes. On keratinocytes, it is suggested to be a critical component of the TGF-beta receptor (T beta R) complex. Here it has been shown to specifically interact with both TGF-beta 1 and T beta RI, and generally with T beta RII and betaglycan. These interactions are inhibitory to TGF-beta signaling, likely the result of CD109's ability to promote internalization and degradation of the T beta R complex via caveolar endosomes. In human, mature CD109 is proposed to arise from a 205 kDa precursor that is cleaved intracellularly into an N-terminal 180 kDa mature molecule, and a C-terminal 25 kDa GPI-linked fragment. This occurs at an Arg tetrapeptide motif that is also conserved in mouse. On the cell surface, the 180 and 25 kDa molecules either stay "associated", or the 180 kDa mature molecule dissociates from the fragment, resulting in its solubilization. In either case, 180 kDa CD109 has the potential to be "activated" by proteolytic cleavage, generating either a 150 or 120 kDa form that may participate in covalent binding to immediately adjacent targets. Mouse CD109 is synthesized as a 1442 amino acid (aa) precursor. It contains a 21 aa signal sequence, a C-terminal prosegment (aa 1420-1442), and a 1398 aa intervening region (aa 22-1419) that possesses a potential furin processing site over aa 1271-1274. The definitive mature molecule (aa 22-1270) contains an MG2 domain (aa 129-220), a Cys thioester bond (Cys923-Gln926), and an alpha 2-macroglobulin-like region (aa 961-1197). Over aa 22-1269, mouse CD109 shares 73% and 81% aa sequence identity with human and rat CD109, respectively.

Entrez Gene IDs
135228 (Human); 235505 (Mouse); 363104 (Rat)
Alternate Names
150 kDa TGF-beta-1-binding protein; activated T-cell marker CD109; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 7; CD109 antigen (Gov platelet alloantigens); CD109 antigen; CD109 molecule; CD109; CPAMD7; CPAMD7r150; DKFZp762L1111; FLJ38569; FLJ41966; Gov platelet alloantigens; p180; Platelet-specific Gov antigen; RP11-525G3.1

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