Mouse CD109 Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CD109 in direct ELISAs and Western blots. In direct ELISAs, approximately 65% cross-reactivity with recombinant human CD109 is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant mouse CD109
    Ala22-Ser1269
    Accession # Q8R422
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse CD109 by Western Blot. Western blot shows lysates of C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.1 µg/mL of Sheep Anti-Mouse CD109 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7717) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for CD109 at approximately 170 and 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD109

CD109 (also CPAMD7, p180, r150, Gov antigen and GPI-anchored alpha 2-macroglobulin-related protein) is a 170-180 kDa member of the I39 protease inhibitor/ alpha 2-macroglobulin family of thioester-containing proteins. It is expressed by endothelium, activated platelets and T cells, megakaryocyte lineage stem cells, myoepithalial cells, fibroblasts and keratinocytes. On keratinocytes, it is suggested to be a critical component of the TGF-beta receptor (T beta R) complex. Here it has been shown to specifically interact with both TGF-beta 1 and T beta RI, and generally with T beta RII and betaglycan. These interactions are inhibitory to TGF-beta signaling, likely the result of CD109's ability to promote internalization and degradation of the T beta R complex via caveolar endosomes. In human, mature CD109 is proposed to arise from a 205 kDa precursor that is cleaved intracellularly into an N-terminal 180 kDa mature molecule, and a C-terminal 25 kDa GPI-linked fragment. This occurs at an Arg tetrapeptide motif that is also conserved in mouse. On the cell surface, the 180 and 25 kDa molecules either stay "associated", or the 180 kDa mature molecule dissociates from the fragment, resulting in its solubilization. In either case, 180 kDa CD109 has the potential to be "activated" by proteolytic cleavage, generating either a 150 or 120 kDa form that may participate in covalent binding to immediately adjacent targets. Mouse CD109 is synthesized as a 1442 amino acid (aa) precursor. It contains a 21 aa signal sequence, a C-terminal prosegment (aa 1420-1442), and a 1398 aa intervening region (aa 22-1419) that possesses a potential furin processing site over aa 1271-1274. The definitive mature molecule (aa 22-1270) contains an MG2 domain (aa 129-220), a Cys thioester bond (Cys923-Gln926), and an alpha 2-macroglobulin-like region (aa 961-1197). Over aa 22-1269, mouse CD109 shares 73% and 81% aa sequence identity with human and rat CD109, respectively.

  • Entrez Gene IDs:
    135228 (Human); 235505 (Mouse); 363104 (Rat)
  • Alternate Names:
    150 kDa TGF-beta-1-binding protein; activated T-cell marker CD109; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 7; CD109 antigen (Gov platelet alloantigens); CD109 antigen; CD109 molecule; CD109; CPAMD7; CPAMD7r150; DKFZp762L1111; FLJ38569; FLJ41966; Gov platelet alloantigens; p180; Platelet-specific Gov antigen; RP11-525G3.1
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