Detection of CD11c in Mouse Splenocytes by Flow Cytometry.
Mouse splenocytes were stained with Hamster Anti-Mouse CD11c Monoclonal Antibody (Catalog # MAB69501) followed by Phycoerythrin-conjugated Anti-Hamster IgG Secondary Antibody (Catalog # F0120) and Rat Anti-Mouse MHC class II (I‑A/I‑E) APC‑conjugated Monoclonal Antibody (Catalog # FAB6118A). Quadrant markers were set based on normal hamster IgG control antibody staining.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
also known as the Integrin alpha X subunit, is a 150 kDa type I
transmembrane protein that noncovalently heterodimerizes with the beta 2
subunit (CD18) to form alpha X/ beta 2, also known as p150/p95 and complement
receptor type 4 (CR4). Integrin alpha X beta 2 is expressed on macrophages,
dendritic cells, hairy cell leukemias and some other leukocyte subsets.
The 1097 aa mouse CD11c extracellular domain shares 71% and 87% amino
acid (aa) identity with human and rat CD11c, respectively. One potential
alpha X isoform is truncated at aa 828. Some adhesion partners of alpha X beta 2 are
shared with alpha M beta 2/CD11b/CD18 (Complement iC3b, ICAMs, vWF and Fibrinogen)
while others (Osteopontin, Thy-1, Plasminogen, Heparin) are unique.
Unlike alpha M beta 2, it is not constitutively active. alpha X beta 2 adhesion mediates
proliferation, degranulation, chemotactic migration, and phagocytosis of
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