Key Product Details

Species Reactivity

Mouse

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 342705
View all CD160 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CD160
Gly28-Ser160
Accession # AAH21596

Specificity

Detects mouse CD160 in direct ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse CD160 Antibody

CD160 antibody in Mouse Splenocytes by Immunocytochemistry (ICC).

CD160 in Mouse Splenocytes.

CD160 was detected in immersion fixed CD8+mouse splenocytes using Rat Anti-Mouse CD160 Monoclonal Antibody (Catalog # MAB38991) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Rat IgG Secondary Antibody (yellow; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Mouse CD160 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed CD8+ mouse splenocytes

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD160

CD160 (also Natural killer cell receptor BY55) is a 16 kDa (predicted) member of the Ig superfamily (1‑4). In mouse, it is expressed principally on nonmyeloid hematopoietic cells. These include CD3+ NK1.1 cells, CD8+ TEM and TCM T cells, CD8 alpha + IELs, NKT cells, CD8‑ gamma delta TCR T cells, and vascular endothelial cells (1, 5‑7). Mouse CD160 has been identified as a 20‑21 kDa GPI‑linked glycoprotein (4, 5). It is synthesized as a preproprotein that is 185 amino acids (aa) in length. The precursor contains a 27 aa signal sequence, a 133 aa mature molecule that shows one 98 aa V‑type Ig‑like domain (aa 28‑125), and a 25 aa prosegment that is cleaved to generate a GPI‑linkage at Ser160. Mouse GPI‑linked CD160 is known to be cleaved by phospholipase C, and this generates a 40 kDa (presumably dimeric) band in SDS‑PAGE (5). One alternative splice form for mouse CD160 is reported to show a deletion of aa 137‑180. This may generate a soluble molecule (5; GenBank Accession # NP_001156969). Mature mouse CD160 shares 63% and 88% aa identity with human and rat CD160, respectively. In mouse, CD160 is reported to bind to bind to HVEM/TNFRSF14, and both classical and non‑classical MHC Class I molecules (5, 8). MHC‑I proteins recognized by CD160 include Dd, Kb, Qa‑1b and CD1d (5). Upon engagement, the effects of CD160 ligation appear to be context dependent. When expressed on endothelial cells, CD160 binding to human HLA‑G1 initiates apoptosis, and thus impacts angiogenesis (6). When expressed on NK1.1 cells, mouse CD160 ligation alone has no effect; when combined with NK1.1 antigen stimulation, CD160 decreases NK cell IFN‑ gamma secretion. Relative to cytotoxicity, NK cell activity is positively correlated with the presence of CD160 (5).

References

  1. Cai, G. & G.J. Freeman (2009) Immunol. Rev. 229:244.
  2. del Rio, M.L. et al. (2010) J. Leukoc. Biol. 87:223.
  3. Maiza, H. et al. (1993) J. Exp. Med. 178:1121.
  4. Anumanthan, A. et al. (1998) J. Immunol. 161:2780.
  5. Maeda, M. et al. (2005) J. Immunol. 175:4426.
  6. Fons, P. et al. (2006) Blood 108:2608.
  7. Tsujimura, K. et al. (2006) Immunol. Lett. 48:106.
  8. Cheung, T.C. et al. (2009) Proc. Natl. Acad. Sci USA 106:6244.

Alternate Names

BY55, CD160, NK1, NK28

Entrez Gene IDs

11126 (Human); 54215 (Mouse); 502585 (Rat); 102117412 (Cynomolgus Monkey)

Gene Symbol

CD160

UniProt

AAH21596

Additional CD160 Products

Product Documents for Mouse CD160 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse CD160 Antibody

For research use only

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