Detects mouse CD161/NK1.1 expressed specifically in the C57/B6 mouse strain. No detection of CD161/NK1.1 was observed in the BALB/c mouse strain. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse (rm) KLRB-1B is observed.
Monoclonal Rat IgG2A Clone # 694370
Protein A or G purified from hybridoma culture supernatant
Detection of CD161/NK1.1 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse NKp46/NCR1 PE‑conjugated Monoclonal Antibody (Catalog # FAB22252P) and either (A) Rat Anti-Mouse CD161/NK1.1 Monoclonal Antibody (Catalog # MAB7614) or (B) Rat IgG2A Isotype Control (Catalog # MAB006) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
KLRB1C, also known as NK1.1, CD161c, Ly-55, and NKR-P1c, is a 28 kDa type 2 transmembrane protein in the killer cell lectin-like receptor family. CD161 is expressed as a disulfide-linked homodimer on the surface of NK cells and subpopulations of NKT, CD4+, CD8+, and gamma /δ T cells. Its cross-linking on NK cells induces cytolytic activity, and on CD4 cells it promotes IgE production and the expansion of Th2 responses. Mature mouse CD161 consists of a 45 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 157 aa extracellular domain with one C-type lectin domain.
Entrez Gene IDs:
CD161 antigen; CD161; CD161/NK1.1; CD161c; CLEC5BC-type lectin domain family 5 member B; HNKR-P1a; killer cell lectin-like receptor subfamily B member 1; killer cell lectin-like receptor subfamily B, member 1; Klrb1c; Ly55c; Ly-59; Natural killer cell surface protein P1A; NKR-P1; NKR-P1AMGC138614; NKRP1ANKR
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