CD229, also known as T lymphocyte surface antigen Ly-9, is a type I transmembrane protein belonging to the immunoglobulin superfamily. It is also a member of the CD150/SLAM receptor family and is expressed on T and B lymphocytes. Mouse CD229 contains 2 Ig-like C2-type domains and 2 Ig-like V-type domains in its extracellular region. Two mouse alleles that differ in 6 extracellular amino acid residues have been reported. Human and mouse CD229 share 60% amino acid sequence identity in their extracellular regions.
Mouse CD229/SLAMF3 Biotinylated Antibody
R&D Systems | Catalog # BAF2555
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot
Label
Biotin
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD229/SLAMF3
Lys48-Phe454
Accession # AAH95921
Lys48-Phe454
Accession # AAH95921
Specificity
Detects mouse CD229/SLAMF3 in Western blots. In Western blots, approximately 15% cross-reactivity with recombinant human CD229 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Mouse CD229/SLAMF3 Biotinylated Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse CD229/SLAMF3
Sample: Recombinant Mouse CD229/SLAMF3
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: CD229/SLAMF3
Long Name
SLAM Family Member 3
Alternate Names
CD229, Ly9, SLAMF3
Gene Symbol
LY9
UniProt
Additional CD229/SLAMF3 Products
Product Documents for Mouse CD229/SLAMF3 Biotinylated Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse CD229/SLAMF3 Biotinylated Antibody
For research use only
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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